Abstract
Radiation mutagenesis could provide new drought-tolerant lines for selection purposes in sustainable agriculture. Drought tolerance and yield stability are closely related to co** with oxidative stress, which occurs at severe/prolonged water deprivation. In this study, the response of a newly generated winter wheat mutant line M181/1338K to severe drought stress at seedling stage (3–4th leaf) was compared to that of two established varieties—Guinness (drought tolerant) and Farmer (drought sensitive). Oxidative damage and antioxidant status analyses were performed on second leaves of control, stressed (45–46% leaf water deficit) and recovered plants. Genotypes exhibited similar pattern of stress response, comprising proline accumulation, rise in hydrogen peroxide content and oxidative damage to membrane lipids, increase in total antioxidant and antiradical activities, in phenolic and flavonoid content, in ascorbate and glutathione pools, mobilization of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX) enzyme isoforms. Farmer responded to severe water stress with the highest levels of oxidative damage to membranes, proline accumulation, and glutathione content, and slower normalization of the studied parameters upon recovery. Guinness presented a better control of oxidative membrane damage and it the highest accumulation of flavonoids under drought. The new mutant line M181/1338K had similarities with Guinness in its response to severe water stress, such as the same proline and glutathione levels. Unlike Guinness, the mutant genotype had more pronounced oxidative damage to membranes, along with higher POX activities, and tended to accumulate less flavonoids under drought, which could be regarded as secondary effects of the induced mutagenesis.
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Abbreviations
- AO:
-
Total antioxidant activity
- AR:
-
Antiradical activity
- ASC:
-
Ascorbic acid
- CAT:
-
Catalase
- DPPH:
-
2,2-Diphenyl-1-picrylhydrazyl
- DW:
-
Dry weight
- FRAP:
-
Ferric reducing antioxidant power assay
- FW:
-
Fresh weight
- GSSG:
-
Oxidized glutathione
- GSH:
-
Reduced glutathione
- MDA:
-
Malondialdehyde
- POX:
-
Peroxidase
- ROS:
-
Reactive oxygen species
- RWC:
-
Relative water content
- SOD:
-
Superoxide dismutase
- TW:
-
Weight at full turgidity
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Acknowledgements
This work was supported in part by project BUL 5014, IAEA—Vienna, and by project BG05M2OP001-2.002-0001 (D.P.) of the Ministry of Science and Education, Bulgaria. The authors are grateful to their colleague assoc. prof. Irina Vaseva, who helped for substantial improvement of the English expression.
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Kirova, E., Pecheva, D. & Simova-Stoilova, L. Drought response in winter wheat: protection from oxidative stress and mutagenesis effect. Acta Physiol Plant 43, 8 (2021). https://doi.org/10.1007/s11738-020-03182-1
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DOI: https://doi.org/10.1007/s11738-020-03182-1