Abstract
Mutation breeding is an alternative method for develo** agriculturally important crops. A large set of Sagittario bread wheat seeds (Triticum aestivum L. cv.) were exposed to gamma ray irradiation (200 Gy) to obtain drought-tolerant mutant lines. To study drought tolerance, polyethylene glycol (PEG) 6000 was applied to the M2 and M3 individuals under in vitro conditions. Except in well-watered (100%) control plants, drought stress was triggered by a 50% decrease in the irrigation water applied to M3 and M4 plants in a greenhouse. Afterwards, 11 candidate drought-tolerant lines were obtained at the M4 stage and subjected to molecular analysis. The highest percentage of polymorphisms (72.4%) was detected with Retrotransposons Microsatellite Amplified Polymorphism (REMAP) markers followed by Inter-Simple Sequence Repeat (ISSR; 62.07%) and Inter-Retrotransposon Amplified Polymorphism (IRAP) markers (52.94%). A dendrogram tree and a principal coordinate analysis (PCoA) plot classified the experimental samples into three distinctive groups. Additionally, the activities of several antioxidant enzymes were evaluated in both vegetative and flowering stages, and mutant lines showing the highest biochemical performance under stress were detected in the same group through phylogenetic analysis. Gamma ray irradiation was used to improve drought-tolerant wheat lines for forward/reverse genomic studies and marker-assisted selection in crops.
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Acknowledgements
This research was supported by the Research Fund of Istanbul University Project Numbers: 31725, 49463, and 55145 to AS. Experimental design, data analysis, and writing the draft of the manuscript were performed by AS. The construction of mutant wheat populations and the field experiments were performed by IO. OY and SA helped detect irradiation doses of the Sagittario cultivar for construction of the mutant population. The final manuscript was read and approved by all of the authors. The authors thank Dr. Aysen YUMURTACI for valuable critiques and editing.
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Sen, A., Ozturk, I., Yaycili, O. et al. Drought Tolerance in Irradiated Wheat Mutants Studied by Genetic and Biochemical Markers. J Plant Growth Regul 36, 669–679 (2017). https://doi.org/10.1007/s00344-017-9668-8
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DOI: https://doi.org/10.1007/s00344-017-9668-8