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Enhanced functional characteristics and digestibility of blends of hemp protein hydrolysate and pea protein isolate

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Abstract

Plant-based proteins show poor functional properties and when a balanced source of amino acids is required, it cannot be provided only by a single source of plant-based protein. This study aims to enhance the functional and digestibility properties of hemp seed meal (HSM) protein by hydrolyzation (HPH) through ultrasonication and enzymatic treatment. The blending of HPH with pea protein isolate (PPI) was conducted to improve the amino acid profile. During ultrasonication, the protein content was significantly affected (p < 0.05) by ultrasonication time and solid-to-solvent ratio but not by the amplitude. Degree of hydrolysis was significantly (p < 0.05) affected by all three variables. Optimal hydrolysate was prepared by ultrasonication at 80% amplitude for 10 min and solid-to-solvent ratio of 1:30, with a protein content of 64.57% and a degree of hydrolysis (DH) of 6.25%. Under these conditions, papain treated HPH exhibited an increase in protein concentration (84.2%) and DH (17.3%). The blend of HPH and PPI in the ratio of 1:1 showed improved protein content (85.5%) with significant (p < 0.05) increase in solubility (72.8% at pH 7). This blend showed a good emulsifying activity index (33.4 m2/g) emulsifying stability (35.3 min), oil holding capacity (2 g/g), water holding capacity (1.8 g/g), foaming capacity (195.8%), and foaming stability (85.2%). This blend also displayed a balanced amino acid profile, with methionine and lysine contents of 1.63 mg/g and 5.88 mg/g, respectively. The blend of PPI:HPH (1:1) showed integration of HPH into the PPI matrix, due water bridging between protein particle as observed by scanning electron microscopy (SEM). The amide I at 1627.6 cm−1 in HSM shifted to 1633.7 cm−1 in HPH and amid II at 1518.7 cm−1 in HSM shifted to 1535.3 cm−1 in HPH indicating a change in secondary structure of HSM due to ultrasonication and enzymatic hydrolysis. In Vitro protein digestibility of 1:1 blend was also higher compared to other samples. The blend of HPH and PPI blend have good potential for creating nutritionally enhanced and functionally superior plant-based food products.

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References

  1. H. Chen, H. Wooten, L. Thompson, K. Pan, Nanoparticles of casein micelles for encapsulation of food ingredients, in Biopolymer Nanostructures for Food Encapsulation Purposes. (Elsevier, Amsterdam, 2019), pp.39–68

    Chapter  Google Scholar 

  2. G. Wu, C.J. Meininger, Regulation of nitric oxide synthesis by dietary factors. Annu. Rev. Nutr.. Rev. Nutr. 22(1), 61–86 (2002)

    Article  CAS  Google Scholar 

  3. X.-S. Wang, C.-H. Tang, X.-Q. Yang, W.-R. Gao, Characterization, amino acid composition and in vitro digestibility of hemp (Cannabis sativa L.) proteins. Food Chem. 107(1), 11–18 (2008)

    Article  CAS  Google Scholar 

  4. Q. Wang, Y.L. **ong, Processing, nutrition, and functionality of hempseed protein: a review. Comp. Rev. Food Sci. Food Saf. 18(4), 936–952 (2019)

    Article  CAS  Google Scholar 

  5. C. Ajibola, Structural and functional properties of hemp seed storage proteins. (2020).

  6. H. Korhonen, A. Pihlanto, Food-derived bioactive peptides-opportunities for designing future foods. Curr. Pharm. Des.. Pharm. Des. 9(16), 1297–1308 (2003)

    Article  CAS  Google Scholar 

  7. A.G.B. Wouters, I. Rombouts, E. Fierens, K. Brijs, J.A. Delcour, Relevance of the functional properties of enzymatic plant protein hydrolysates in food systems. Comp. Rev. Food Sci. Food Saf. 15(4), 786–800 (2016)

    Article  CAS  Google Scholar 

  8. R.R. Balandrán-Quintana, A.M. Mendoza-Wilson, G.R.-C. Montfort, J.Á. Huerta-Ocampo, Plant-based proteins, in Proteins: Sustainable Source, Processing and Applications. ed. by C.M. Galanakis (Elsevier, Amsterdam, 2019), pp.97–130

    Chapter  Google Scholar 

  9. A.-F. Monnet, K. Laleg, C. Michon, V. Micard, Legume enriched cereal products: A generic approach derived from material science to predict their structuring by the process and their final properties. Trends Food Sci. Technol. 86, 131–143 (2019)

    Article  CAS  Google Scholar 

  10. M. C. Tulbek, Pulse flours as functional ingredients. IUFOST Annual Conference, Montreal QC, Canada. (2014)

  11. S.-W. Yin, C.-H. Tang, J.-S. Cao, E.-K. Hu, Q.-B. Wen, X.-Q. Yang, Effects of limited enzymatic hydrolysis with trypsin on the functional properties of hemp (Cannabis sativa L.) protein isolate. Food Chem. 106(3), 1004–1013 (2008)

    Article  CAS  Google Scholar 

  12. Z.X. Lu, J.F. He, Y.C. Zhang, D.J. Bing, Composition, physicochemical properties of pea protein and its application in functional foods. Crit. Rev. Food Sci. Nutr.Nutr. 60(15), 2593–2605 (2020)

    Article  CAS  Google Scholar 

  13. M. Umar, U.R. Ruktanonchai, D. Makararpong, A.K. Anal, Compositional and functional analysis of freeze-dried bovine skim colostrum powders. J. Food Meas. Charact. (2023). https://doi.org/10.2139/ssrn.4226729

    Article  Google Scholar 

  14. S. Cui, S. Zhang, S. Ge, L. **ong, Q. Sun, Green preparation and characterization of size-controlled nanocrystalline cellulose via ultrasonic-assisted enzymatic hydrolysis. Ind. Crops Prod. 83, 346–352 (2016)

    Article  CAS  Google Scholar 

  15. P.M. Nielsen, D. Petersen, C. Dambmann, Improved method for determining food protein degree of hydrolysis. J. Food Sci. 66(5), 642–646 (2001)

    Article  CAS  Google Scholar 

  16. R.P. Bebartta, M. Umar, U.R. Ruktanonchai, A.K. Anal, Development of cyrodesiccated micronized protein conglomerates; effect of maltodextrin on stability, functionality and digestibility. Food Process. Eng. 46(7), e14350 (2023). https://doi.org/10.1111/jfpe.14350

    Article  CAS  Google Scholar 

  17. S. Chandra, S. Singh, D. Kumari, Evaluation of functional properties of composite flours and sensorial attributes of composite flour biscuits. J. Food Sci. Technol. 52, 3681–3688 (2015)

    CAS  PubMed  Google Scholar 

  18. M. Verni, Determination of the content of free amino acids and their profiling, in Basic Methods and Protocols on Sourdough. ed. by M. Gobbetti, C.G. Rizzello (Springer, New York, 2024), pp.71–79

    Chapter  Google Scholar 

  19. M. Umar, U.R. Ruktanonchai, D. Makararpong, A. Panya, A.K. Anal, Fabrication of biopolymeric nanoparticles of colostrum whey-caseinate, characterization, and in vitro digestibility. J. Food Eng. 369, 111933 (2024)

    Article  CAS  Google Scholar 

  20. M. Umar, U.R. Ruktanonchai, D. Makararpong, A.K. Anal, Effects of pH and concentrations of colostrum whey and caseinate on fabrication of nanoparticles and evaluation of their techno-functionalities and in vitro digestibility. J. Food Meas. Charact. 17, 1–12 (2023)

    Article  CAS  Google Scholar 

  21. A.P. Bonto, R.N. Tiozon Jr., N. Sreenivasulu, D.H. Camacho, Impact of ultrasonic treatment on rice starch and grain functional properties: a review. Ultrason. Sonochem.. Sonochem. 71, 105383 (2021)

    Article  CAS  Google Scholar 

  22. M.A. Malik, H.K. Sharma, C.S. Saini, High intensity ultrasound treatment of protein isolate extracted from dephenolized sunflower meal: effect on physicochemical and functional properties. Ultrason. Sonochem.. Sonochem. 39, 511–519 (2017)

    Article  CAS  Google Scholar 

  23. B. Nazari, M.A. Mohammadifar, S. Shojaee-Aliabadi, E. Feizollahi, L. Mirmoghtadaie, Effect of ultrasound treatments on functional properties and structure of millet protein concentrate. Ultrason. Sonochem. Sonochem. 41, 382–388 (2018)

    Article  CAS  Google Scholar 

  24. Q. Zhao, T. **e, X. Hong, Y. Zhou, L. Fan, Y. Liu, J. Li, Modification of functional properties of perilla protein isolate by high-intensity ultrasonic treatment and the stability of o/w emulsion. Food Chem. 368, 130848 (2022)

    Article  CAS  PubMed  Google Scholar 

  25. A.B. Stefanović, J.R. Jovanović, B.D. Balanč, N.Ž Šekuljica, S.M.J. Tanasković, M.B. Dojčinović, Z.D. Knežević-Jugović, Influence of ultrasound probe treatment time and protease type on functional and physicochemical characteristics of egg white protein hydrolysates. Poult. Sci.. Sci. 97(6), 2218–2229 (2018)

    Article  Google Scholar 

  26. S. Jung, C. Roussel-Philippe, J.L. Briggs, P.A. Murphy, L.A. Johnson, Limited hydrolysis of soy proteins with endo-and exoproteases. J. Am. Oil Chem. Soc. 81(10), 953 (2004)

    Article  CAS  Google Scholar 

  27. J. Miedzianka, A. Pęksa, M. Pokora, E. Rytel, A. Tajner-Czopek, A. Kita, Improving the properties of fodder potato protein concentrate by enzymatic hydrolysis. Food Chem. 159, 512–518 (2014)

    Article  CAS  PubMed  Google Scholar 

  28. Z. Ding, F. Jiang, K. Liu, F. Gong, Y. Liu, Z. Zheng, Y.-J. Xu, Structural and functional characteristics of hemp protein isolate-pullulan polysaccharide glycosylation conjugate in an aqueous model system. Foods 12(7), 1416 (2023)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. M. Hamouda, A. Sboui, A. Omrani, M. Dbara, S. Zaidi, M. Hammadi, T. Khorchani, Effect of thermal treatments on the fatty acids composition, antioxidant and anti-inflammatory properties of camel milk. J. Food Eng. Technol. 11(2), 55–61 (2022)

    Article  Google Scholar 

  30. P. Moll, H. Salminen, E. Griesshaber, C. Schmitt, J. Weiss, Homogenization improves foaming properties of insoluble pea proteins. J. Food Sci. 87(10), 4622–4635 (2022)

    Article  CAS  PubMed  Google Scholar 

  31. J.M.R. Patino, C.C. Sánchez, M.R.R. Niño, Implications of interfacial characteristics of food foaming agents in foam formulations. Adv. Coll. Interface. Sci. 140(2), 95–113 (2008)

    Article  Google Scholar 

  32. S.A. Malomo, R.E. Aluko, A comparative study of the structural and functional properties of isolated hemp seed (Cannabis sativa L.) albumin and globulin fractions. Food Hydrocolloids 43, 743–752 (2015)

    Article  CAS  Google Scholar 

  33. P.G. Kougias, K. Boe, E.S. Einarsdottir, I. Angelidaki, Counteracting foaming caused by lipids or proteins in biogas reactors using rapeseed oil or oleic acid as antifoaming agents. Water Res. 79, 119–127 (2015)

    Article  CAS  PubMed  Google Scholar 

  34. I.A. Wani, D.S. Sogi, B.S. Gill, Physico-chemical and functional properties of native and hydrolysed protein isolates from Indian black gram (Phaseolus mungo L.) cultivars. LWT 60(2), 848–854 (2015)

    Article  CAS  Google Scholar 

  35. M. del Mar Yust, J. Pedroche, M. del Carmen Millán-Linares, J.M. Alcaide-Hidalgo, F. Millán, Improvement of functional properties of chickpea proteins by hydrolysis with immobilised alcalase. Food Chem. 122(4), 1212–1217 (2010)

    Article  Google Scholar 

  36. S.A. Malomo, R.E. Aluko, Conversion of a low protein hemp seed meal into a functional protein concentrate through enzymatic digestion of fibre coupled with membrane ultrafiltration. Innov. Food Sci. Emerg. Technol. 31, 151–159 (2015)

    Article  CAS  Google Scholar 

  37. R.K. Bhaskaracharya, S. Kentish, M. Ashokkumar, Selected applications of ultrasonics in food processing. Food Eng. Rev. 1, 31–49 (2009)

    Article  CAS  Google Scholar 

  38. H. Twinomuhwezi, C.G. Awuchi, M. Rachael, Comparative study of the proximate composition and functional properties of composite flours of amaranth, rice, millet, and soybean. Am. J. Food Sci. Nutr. 6(1), 6–19 (2020)

    Google Scholar 

  39. D. Konieczny, A.K. Stone, D.R. Korber, M.T. Nickerson, T. Tanaka, Physicochemical properties of enzymatically modified pea protein-enriched flour treated by different enzymes to varying levels of hydrolysis. Cereal Chem. 97(2), 326–338 (2020)

    Article  CAS  Google Scholar 

  40. G. Sonali, C. Nirmala, D. Saroj et al., Genetic variability, correlation and path analysis studies in pea (Pisum sativum L.). Crop Res. (Hisar) 38(1/3), 179–183 (2009)

    Google Scholar 

  41. X. Kong, H. Zhou, H. Qian, Enzymatic hydrolysis of wheat gluten by proteases and properties of the resulting hydrolysates. Food Chem. 102(3), 759–763 (2007)

    Article  CAS  Google Scholar 

  42. Y. Luo, K. Pan, Q. Zhong, Physical, chemical and biochemical properties of casein hydrolyzed by three proteases: partial characterizations. Food Chem. 155, 146–154 (2014)

    Article  CAS  PubMed  Google Scholar 

  43. A. Connolly, C.O. Piggott, R.J. FitzGerald, Technofunctional properties of a brewers’ spent grain protein-enriched isolate and its associated enzymatic hydrolysates. LWT 59(2), 1061–1067 (2014)

    Article  CAS  Google Scholar 

  44. A.M. Ghribi, I.M. Gafsi, A. Sila, C. Blecker, S. Danthine, H. Attia, A. Bougatef, S. Besbes, Effects of enzymatic hydrolysis on conformational and functional properties of chickpea protein isolate. Food Chem. 187, 322–330 (2015)

    Article  Google Scholar 

  45. C. Ozuna, I. Paniagua-Martínez, E. Castaño-Tostado, L. Ozimek, S.L. Amaya-Llano, Innovative applications of high-intensity ultrasound in the development of functional food ingredients: production of protein hydrolysates and bioactive peptides. Food Res. Int. 77, 685–696 (2015)

    Article  CAS  Google Scholar 

  46. M. Liu, J.A. Toth, M. Childs, L.B. Smart, A. Abbaspourrad, Composition and functional properties of hemp seed protein isolates from various hemp cultivars. J. Food Sci. 88(3), 942–951 (2023)

    Article  CAS  PubMed  Google Scholar 

  47. J. De Meutter, E. Goormaghtigh, Evaluation of protein secondary structure from FTIR spectra improved after partial deuteration. Eur. Biophys. J.Biophys. J. 50, 613–628 (2021)

    Article  Google Scholar 

  48. F. Chemat, N. Rombaut, A.G. Sicaire, A. Meullemiestre, A.S. Fabiano-Tixier, M. Abert-Vian, Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrason. Sonochem. 34, 540–560 (2017). https://doi.org/10.1016/j.ultsonch.2016.06.035

    Article  CAS  PubMed  Google Scholar 

  49. D. M. Kadam, M. Sharma, S. Chadha, and R. A. Wilson, Effect of particle size of foam mat dried tomato juice powder on various physical and sensory attributes. 2012 Dallas, Texas, July 29-August 1, 2012, 1. (2012).

  50. K. Sarabandi, S.H. Peighambardoust, A.S. Mahoonak, S.P. Samaei, Effect of carrier types and compositions on the production yield, microstructure and physical characteristics of spray dried sour cherry juice concentrate. J. Food Meas. Charact. 11(4), 1602–1612 (2017)

    Article  Google Scholar 

  51. T. Hu, J. Chen, X. He, Y. Tang, J. Sun, C. Liu, T. Dai, Complex plant protein prepared from rice protein and pea protein: improve the thermal stability of betanin. Food Res. Int. 164, 112341 (2023)

    Article  CAS  PubMed  Google Scholar 

  52. A.G.A. Sá, Y.M.F. Moreno, B.A.M. Carciofi, Plant proteins as high-quality nutritional source for human diet. Trends Food Sci. Technol. 97, 170–184 (2020)

    Article  Google Scholar 

  53. L.M. Jiménez-Munoz, G.M. Tavares, M. Corredig, Design future foods using plant protein blends for best nutritional and technological functionality. Trends Food Sci. Technol. 113, 139–150 (2021)

    Article  Google Scholar 

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  1. Department of Food, Agriculture and Bioresources, Asian Institute of Technology, Pathum Thani, 12120, Thailand

    Puja Bhetwal, Muhammad Umar & Anil Kumar Anal

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Bhetwal, P., Umar, M. & Anal, A.K. Enhanced functional characteristics and digestibility of blends of hemp protein hydrolysate and pea protein isolate. Food Measure (2024). https://doi.org/10.1007/s11694-024-02722-4

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