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Ethanol–water binary solvent affects phenolic composition and antioxidant ability of Pistacia lentiscus L. fruit extracts: a theoretical versus experimental solubility study

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Abstract

Pistacia lentiscus L. fruits are an excellent source of phenolic compounds endowed with antioxidant activities. Ethanol–water mixtures are in focus for plant extracts preparation due to their acceptability for human consumption. In this study, the effect of ethanol concentration in ethanol/water (EtOH/H2O) extraction solvent on the solubility of target phenolic compounds was firstly predicted using COnductor-like Screening MOdel for Real Solvents (COSMO-RS). Subsequently, the computational results were confirmed experimentally based on extraction yield, phenolic composition, antioxidant activity and RP-HPLC analysis. Extractions were achieved using bead milling by varying EtOH concentrations in order to prepare a suitable extract that could be safely used for food purposes. The obtained results highlighted a good correlation between COSMO-RS simulations and experiments. The highest polyphenol and flavonoid contents (292.04 mg GAE/g DR and 11.07 mg CE/g DR, respectively) were achieved with 70% EtOH, which is in correlation with RP-HPLC quantitative analysis, while the highest anthocyanin content (94.00 mg C3OG/100 g DR) was obtained with 80% EtOH. Concerning antioxidant activity, the best DPPH· radical scavenging activity (IC50 = 2.39 µg/mL) was obtained with 70% EtOH. Reverse Phase High Performance Liquid Chromatography (RP-HPLC) analysis showed that gallic acid was the major compound of Pistacia lentiscus fruits in all EtOH/H2O mixtures. These findings showed that the EtOH/H2O (70/30) mixture is the most suitable to obtain P. lentiscus fruit extracts with the highest phenolic compounds content and antioxidant activity.

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References

  1. A. Khaldi, A. Hamrouni, A. Khorchani, B. Stiti, T.H. Ahn, S.H. Lee, Y.H. Kwon, H.J. Lee, Northern and East Coastal Areas of Tunisia Plants of Mediterranean (Korea National Arboretum, Pocheon, 2013), pp.43–45

    Google Scholar 

  2. C. Mehenni, D. Atmani-Kilani, S. Dumarçay, D. Perrin, P. Gérardin, D. Atmani, J. Food Drug Anal. 24, 653–669 (2016)

    Article  PubMed  PubMed Central  Google Scholar 

  3. M. Harrat, N. Gourine, M. Válega, A.M.S. Silva, M. Yousfi, J. Food Meas. Charact. 14, 1939–1956 (2020)

    Article  Google Scholar 

  4. H. Trabelsi, O.A. Cherif, F. Sakouhi, P. Villeneuve, J. Renaud, N. Barouh, S. Boukchina, P. Mayer, Food Chem. 131, 2–6 (2012)

    Article  Google Scholar 

  5. E. Chaabani, M. Vian, S. Dakhlaoui, S. Bourgou, F. Chemat, R. Ksouri, OCL 26, 25 (2019)

    Article  Google Scholar 

  6. W. Dhifi, N. Jelali, E. Chaabani, M. Béji, S. Fatnassi, S. Omri, W. Mnif, Afr. J. Agric. Res. 8, 16 (2013)

    Google Scholar 

  7. F. Mezni, A. Slama, R. Ksouri, G. Hamdaoui, M.L. Khouja, A. Khaldi, Food Chem. 257, 206–210 (2018)

    Article  CAS  PubMed  Google Scholar 

  8. A. Abdelwahed, I. Bouhlel, I. Skandrani, K. Valenti, M. Kadri, P. Guiraud, R. Steiman, A.M. Mariotte, K. Ghedira, F. Laporte, M.G. Dijoux-Franca, L. Chekir-Ghedira, Chem. Biol. Interact. 165, 1–13 (2007)

    Article  CAS  PubMed  Google Scholar 

  9. W. Bhouri, S. Derbel, I. Skandrani, J. Boubaker, I. Bouhlel, M.B. Sghaier, S. Kilani, A.M. Mariotte, M.G. Dijoux-Franca, K. Ghedira, L. Chekir-Ghedira, Toxicol. In Vitro 24, 509–515 (2010)

    Article  CAS  PubMed  Google Scholar 

  10. L. Longo, A. Scardino, G. Vasapollo, Innov. Food Sci. Emerg. Technol. 8, 360–364 (2007)

    Article  CAS  Google Scholar 

  11. S. Remila, D. Atmani-Kilani, S. Delemasure, J.L. Connat, L. Azib, T. Richard, D. Atmani, Eur. J. Integr. Med. 7, 274–286 (2015)

    Article  Google Scholar 

  12. I.E. Garofulić, V. Kruk, A. Martić, I. Martić, Z. Zorić, S. Pedisić, S. Dragović, V. Dragović-Uzelac, Foods 9, 1556 (2020)

    Article  Google Scholar 

  13. A. Zitouni, N. Belyagoubi-Benhammou, N. Ghembaza, F. Toul, F. Atik-Bekkara, Int. J. Pharmacogn. Phytochem. Res. 8, 627–633 (2016)

    Google Scholar 

  14. D. Belhachat, F. Aid, L. Mekimene, M. Belhachat, Med. J. Nutr. Metab. 10, 273–285 (2017)

    Google Scholar 

  15. R. Hemma, S. Belhadj, C. Ouahchia, F. Saidi, Rev. Agrobiol. 8, 845–852 (2018)

    Google Scholar 

  16. M. Barbouchi, K. Elamrani, M. El Idrissi, M. Choukrad, J. King Saud Univ. 32, 302–306 (2020)

    Article  Google Scholar 

  17. B. Martín-García, S.D. Montijo-Prieto, M. Jiménez-Valera, A. Carrasco-Pancorbo, A. Ruiz-Bravo, V. Verardo, A.M. Gómez-Caravaca, Antioxidants 11, 558 (2022)

    Article  PubMed  PubMed Central  Google Scholar 

  18. A. Carreira-Casais, C. Lourenço-Lopes, P. Otero, M. Carpena, A.G. Pereira, J. Echave, A. Soria-Lopez, F. Chamorro, M.A. Prieto, J. Simal-Gandara, Food Additives (Intech Open, London, 2021). https://doi.org/10.5772/intechopen.100320

    Book  Google Scholar 

  19. A. Sridhar, M. Ponnuchamy, P.S. Kumar, A. Kapoor, D.V.N. Vo, S. Prabhakar, Environ. Chem. Lett. 19, 3409–3443 (2021)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. S. Catena, N. Rakotomanomana, P. Zunin, R. Boggia, F. Turrini, F. Chemat, Ultrason. Sonochem. (2020). https://doi.org/10.1016/j.ultsonch.2020.105231

    Article  PubMed  Google Scholar 

  21. H.N. Özbek, F. Halahlih, F. Göğüş, D.K. Yanık, H. Azaizeh, Waste Biomass Valoriz. (2018). https://doi.org/10.1007/s12649-018-0512-6

    Article  Google Scholar 

  22. F. Chemat, N. Rombaut, A.S. Fabiano-Tixier, J.T. Pierson, A. Bily, Green Extraction of Natural Products: Theory and Practice (Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, 2015), p.363

    Book  Google Scholar 

  23. K. Ameer, H.M. Shahbaz, J.H. Kwon, Compr. Rev. Food Sci. Food Saf. (2017). https://doi.org/10.1111/1541-4337.12253

    Article  PubMed  Google Scholar 

  24. Q.W. Zhang, L.G. Lin, W.C. Ye, Chin. Med. 13, 20 (2018)

    Article  PubMed  PubMed Central  Google Scholar 

  25. R. Kalra, X.A. Conlan, M. Goel, Front. Chem. 8, 369 (2020)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. G. Cravotto, A. Binello, L. Orio, Agro Food Ind. Hi-Tech 22, 24–36 (2011)

    Google Scholar 

  27. A. Meullemiestre, C. Breil, M. Abert-Vian, F. Chemat, Bioresour. Technol. 211, 190–199 (2016)

    Article  CAS  PubMed  Google Scholar 

  28. H.K. Ravi, C. Breil, M.A. Vian, F. Chemat, P.R. Venskutonis, ACS Sustain. Chem. Eng. 6, 4185–4193 (2018)

    Article  CAS  Google Scholar 

  29. E. Chaabani, M. Abert Vian, R. Bott, C. Ginies, C. Defoort, R. Ksouri, F. Chemat, Sep. Sci. Technol. (2019). https://doi.org/10.1080/01496395.2019.1574821

    Article  Google Scholar 

  30. J. Dai, R.J. Mumper, Molecules 15, 7313–7352 (2010)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. K. Waszkowiak, A. Gliszczyńska-Świgło, Eur. Food Res. Technol. 242, 777–786 (2016)

    Article  CAS  Google Scholar 

  32. J.O. Chaves, M.C. de Souza, L.C. da Silva, D. Lachos-Perez, P.C. Torres-Mayanga, A.P. da Fonseca Machado, T. Forster-Carneiro, M. Vázquez-Espinosa, A.V. González-de-Peredo, G.F. Barbero, M.A. Rostagno, Front. Chem. 8, 507887 (2020)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. J. Shi, H. Nawaz, J. Pohorly, G. Mittal, Y. Kakuda, Y. Jiang, Food Rev. Int. 21, 139–166 (2005)

    Article  CAS  Google Scholar 

  34. Z.S. Zhang, D. Li, L.J. Wang, N. Ozkan, X.D. Chen, Z.H. Mao, H.Z. Yang, Sep. Purif. Technol. 57, 17–24 (2007)

    Article  CAS  Google Scholar 

  35. F. Chemat, Zill-e-Huma, M.K. Khan, Ultrason. Sonochem. 18, 813–835 (2011)

    Article  CAS  PubMed  Google Scholar 

  36. M. Muzolf-Panek, K. Stuper-Szablewska, J. Food Meas. Charact. 15, 4561–4574 (2021)

    Article  Google Scholar 

  37. N. Vural, Ö.A. Cavuldak, M.A. Akay, R.E. Anlı, J. Food Meas. Charact. 14, 1286–1305 (2020)

    Article  Google Scholar 

  38. K. Robards, J. Chromatogr. A 1000, 657–691 (2003)

    Article  CAS  PubMed  Google Scholar 

  39. M. Jacotet-Navarro, M. Laguerre, A.S. Fabiano-Tixier, M. Tenon, N. Feuillère, A. Bily, F. Chemat, Electrophoresis 39, 1946–1956 (2018)

    Article  CAS  Google Scholar 

  40. V. Singleton, J. Rossi, Am. J. Enol. Vitic. 16, 144–158 (1965)

    Article  CAS  Google Scholar 

  41. V.X. Dewanto, K. Wu, K. Adom, R.H. Liu, J. Agric. Food Chem. 50, 3010–3014 (2002)

    Article  CAS  PubMed  Google Scholar 

  42. J. Lee, R.W. Durst, R.E. Wrolstad, J. AOAC Int. 88, 1269–1278 (2005)

    Article  CAS  PubMed  Google Scholar 

  43. H. Hanato, T. Kagawa, J. Yasuhara, T. Okuda, Chem. Pharm. Bull. 36, 1090–1097 (1988)

    Google Scholar 

  44. A. Klamt, Fluid Phase Equilib. 206, 223–235 (2003)

    Article  CAS  Google Scholar 

  45. C. Breil, A. Meullemiestre, M. Vian, F. Chemat, Molecules 21, 196 (2016). https://doi.org/10.3390/molecules21020196

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  46. K. Bundeesomchok, A. Filly, N. Rakotomanomana, P. Panichayupakaranant, F. Chemat, LWT 65, 297–303 (2016)

    Article  CAS  Google Scholar 

  47. H.K. Ravi, C. Breil, M.A. Vian, F. Chemat, P.R. Venskutonis, A.C.S. Sustain, Chem. Eng. 6, 4185–4193 (2018)

    CAS  Google Scholar 

  48. J.E. Cacace, G. Mazza, J. Food Sci. 68, 240–248 (2003)

    Article  CAS  Google Scholar 

  49. G. Spigno, L. Tramelli, D.M. De Faveri, J. Food Eng. 8, 200–208 (2007)

    Article  Google Scholar 

  50. Y. Yilmaz, R.T. Toledo, J. Food Compos. Anal. 19, 41–44 (2006)

    Article  CAS  Google Scholar 

  51. M.K. Khan, L. Paniwnyk, S. Hassan, Plant Based “Green Chemistry 2.0”. Green Chemistry and Sustainable Technology (Springer, Singapore, 2019), p.39

    Google Scholar 

  52. A.D. Assefa, R.K. Saini, Y.S. Keum, Food Meas. 11, 364–379 (2017)

    Article  Google Scholar 

  53. E. Milia, S.M. Bullitta, G. Mastandrea, B. Szotáková, A. Schoubben, L. Langhansová, M. Quartu, A. Bortone, S. Eick, Antibiotics 10, 425 (2021)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  54. M. Boulaaba, S. Soninkhishig Tsolmon, R. Ksouri, J. Han, K. Kawada, A. Smaoui, A. Chedly, H. Isoda, Cytotechnology 65, 927–936 (2013)

    Article  PubMed  PubMed Central  Google Scholar 

  55. R. Ksouri, W.M. Ksouri, I. Jallali, A. Debe, C. Magné, I. Hiroko, C. Abdelly, Crit. Rev. Biotechnol. 32, 289–326 (2012)

    Article  CAS  PubMed  Google Scholar 

  56. S. Ben Jannet, N. Hymery, S. Bourgou, A. Jdey, M. Lachaal, C. Magné, R. Ksouri, Biomed. Pharmacother. 90, 375–385 (2017)

    Article  CAS  PubMed  Google Scholar 

  57. M.M. Bratu, S. Birghila, A.S. Popescu, B. Negreanu-Pirjol, T. Negreanu-Pirjol, Bull. Chem. Soc. Ethiop. 32, 1 (2018)

    Article  CAS  Google Scholar 

  58. M.C. Baratto, M. Tattini, C. Galardi, P. Pinelli, A. Romani, F. Visioli, R. Basosi, R. Pogni, Free Radic. Res. 37, 405–412 (2003)

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Laboratoire des Plantes Aromatiques et Médicinales (LPAM), Centre de Biotechnologie de Borj-Cédria, BP 901, 2050, Hammam-Lif, Tunisia

    Emna Chaabani, Iness Bettaieb Rebey, Soumaya Bourgou, Feten Zar Kalai & Riadh Ksouri

  2. Université d’Avignon et des Pays de Vaucluse, INRA, UMR408, GREEN Extraction Team, 84000, Avignon, France

    Emna Chaabani, Maryline Abert Vian & Farid Chemat

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  1. Emna Chaabani
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Correspondence to Iness Bettaieb Rebey.

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Chaabani, E., Abert Vian, M., Bettaieb Rebey, I. et al. Ethanol–water binary solvent affects phenolic composition and antioxidant ability of Pistacia lentiscus L. fruit extracts: a theoretical versus experimental solubility study. Food Measure 17, 4705–4714 (2023). https://doi.org/10.1007/s11694-023-01983-9

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