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Effect of high-pressure ultrasonic extraction on structural characterization and biological activities of polysaccharide from ginger stems and leaves

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Abstract

This study uses response surface methodology to investigate how ultrasonic-assisted extraction conducted under high pressure and no additional pressure affects chemical composition, structural characteristics, and biological activities of polysaccharides from ginger stems and leaves (GSLPs). High-pressure ultrasonic extraction (HUE) was found to obtain a 9.82 ± 0.37% extraction yield of HUE-GSLPs, while ultrasonic extraction with no additional pressurization (UE) obtained an 8.22 ± 0.65% yield of UE-GSLPs. In addition to the superior yield, the HUE-GSLPs had a lower molecular weight and possessed higher contents of total sugar, uronic acid, and sulfate radical. The compositions of the HUE-GSLPs and UE-GSLPs were qualitatively similar, with both featuring Man, Rha, Glc, Gal, Ara, GlcA, and GalA. X-ray diffraction, thermogravimetric analysis, and scanning electron microscope characterizations confirmed that the HUE-GSLPs and UE-GSLPs exhibited similar semi-crystalline structures, thermal properties, and morphologies featuring rod-like and flaky structures. Additionally, the HUE-GSLPs showed better α-glucosidase inhibitory activity with competitive inhibition type than did the UE-GSLPs. Antioxidant activity assays revealed that compared to the UE-GSLPs, the HUE-GSLPs showed stronger DPPH, ABTS, hydroxyl, and superoxide radical scavenging activities, as well as considerable chelating ability and reducing power. Altogether, these results suggest that GSLPs extracted under the correct conditions have great potential to serve extensively as an active agent in the nutrition and cosmetics industries.

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Acknowledgements

This work was supported by the Project of Innovation Team Construction and Condiment Industry Technology System with Modern-mountain characteristic and High-efficiency agriculture in Chongqing (No. 2017[7]), the Project Funded by the Agriculture and Rural Affairs Committee of Chongqing.

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Authors and Affiliations

  1. College of Food Science, Southwest University, 2 Tiansheng Road, Beibei, Chongqing, 400715, People’s Republic of China

    Xuhui Chen, Zhirong Wang, Qingqing Yang & Jianquan Kan

  2. Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing), Ministry of Agriculture, Chongqing, 400715, People’s Republic of China

    Xuhui Chen, Zhirong Wang, Qingqing Yang & Jianquan Kan

  3. Chinese-Hungarian Cooperative Research Centre for Food Science, Chongqing, 400715, People’s Republic of China

    Xuhui Chen, Zhirong Wang, Qingqing Yang & Jianquan Kan

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  1. Xuhui Chen
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XC: Conceptualization; Data curation; Writing—original draft; Formal analysis. ZW: Data curation; Revising—review & editing. QY: Revising—review & editing. JK: Funding acquisition; Project administration; Writing—review & editing.

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Correspondence to Jianquan Kan.

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Chen, X., Wang, Z., Yang, Q. et al. Effect of high-pressure ultrasonic extraction on structural characterization and biological activities of polysaccharide from ginger stems and leaves. Food Measure 16, 85–101 (2022). https://doi.org/10.1007/s11694-021-01159-3

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