Abstract
This study aimed to compare the effects of hot water and vacuum blanching methods on physico-chemical parameter, total phenols (TPC) and flavonoids content (TFC), and antioxidant activity of zucchini slices during 28 days storage. A significant decrease in Chroma parameter was observed over time, on the other hand, no significant differences were observed between the Hue angle either between samples treated with the two blanching methods or during storage. A minimal reduction in the TPC was observed in all samples, independently by the applied treatment, compared to the value of untreated product whereas a more marked reduction in TFC compared to untreated zucchini slices was observed (− 39.91% and − 32.02% for vacuum blanched (ZS8) and hot water blanched (ZB8) zucchini treated for 8 min, respectively, respect the untreated zucchini samples at D0). First-order kinetic model was applied to monitor the effect of the two blanching processes on zucchini slices. The application of vacuum blanching allows for greater retention of bioactive compounds over time with a half-life time (t1/2) of 161 and 47 days for ZS8 versus 87 and 23 days for ZB8, respectively. Samples treated with both blanching procedure for 8 min exhibited a more markedly ABTS radical activity respect untreated one (IC50 values of 17.55 and 14.98 μg/mL for ZS8 and ZB8, respectively vs 20.45 μg/mL for untreated zucchini). In general, the results obtained allow to identify in the vacuum treatment a method applicable to zucchini slices to maintain their qualitative characteristics over time.
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All data and materials are available on request to the corresponding author.
Abbreviations
- ABTS:
-
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sul-phonic acid)
- DPPH:
-
2,2-Diphenyl-1-picrylhydrazyl
- PCA:
-
Principal component analysis
- TPC:
-
Total phenols content
- TFC:
-
Total flavonoids content
- ZB:
-
Hot water blanching
- ZS:
-
Vacuum blanching
References
L.M.J. Carvalho, A.S.M. Smiderle, J.L.V. Carvalho, F.S.N. Cardoso, M.G.B. Koblitz, Assessment of carotenoids in pumpkins after different home cooking conditions. Food Sci. Technol. 34, 365–370 (2014). https://doi.org/10.1590/fst.2014.0058
http://www.italiafruit.net/DettaglioNews/47116/lapprofondimento/le-zucchine-tra-presente-e-futuro-varieta-virosi-e-consumi. Accessed 19 July 2021
S. Mukherjee, P. Chattopadhyay, Whirling bed blanching of potato cubes and its effects on product quality. J. Food Eng. 78, 52–60 (2007). https://doi.org/10.1016/j.jfoodeng.2005.09.001
N. Jacobo-Valenzuela, M.R. Maróstica-Junior, J.D.J. Zazueta-Morales, J.A. Gallegos-Infante, Physicochemical, technological properties, and health-benefits of Cucurbita moschata Duchense vs. Cehualca: a review. Food Res. Int. 44, 2587–2593 (2011). https://doi.org/10.1016/j.foodres.2011.04.039
L. Tejada, L. Buendía-Moreno, A. Villegas, J.M. Cayuela, E. Bueno-Gavilá, P. Gómez, A. Abellán, Nutritional and sensorial characteristics of zucchini (Cucurbita pepo L.) as affected by freezing and the culinary treatment. Int. J. Food Prop. 23, 1825–1833 (2020). https://doi.org/10.1080/10942912.2020.1826512
A.K. Jaiswal, S. Gupta, N. Abu-Ghannam, Kinetic evaluation of color, texture, polyphenols and antioxidant capacity of Irish York cabbage after blanching treatment. Food Chem. 131, 63–72 (2012). https://doi.org/10.1016/j.foodchem.2011.08.032
U.S. Herath, Consumer behavior and attitudes in purchasing vegetables. Agric. Res. Technol. 20, 556123 (2019). https://doi.org/10.19080/ARTOAJ.2019.20.556123
C. Wang, B. Zhang, L. Song, P. Li, Y. Hao, J. Zhang, Assessment of different blanching strategies on quality characteristics and bioactive constituents of Toona sinensis. LWT 130, 109549 (2020). https://doi.org/10.1016/j.lwt.2020.109549
M.I. Cantwell, R.F. Kasmire, Postharvest handling systems: fruits Vegetables, in Postharvest Tecnhology of Horticultural Crops. ed. by A.A. Kader (University of California. Agricultural and Natural Recourses, Los Angles, 2011), pp. 407–422
F. Carvajal, C. Martinez, M. Jamilena, D. Garrido, Differential response of zucchini varieties to low storage temperature. Sci. Hortic. 130, 90–96 (2011). https://doi.org/10.1016/j.scienta.2011.06.016
S. Viña, D. Olivera, C. Marani, R. Ferreyra, A. Mugridge, A. Chaves, R. Mascheroni, Quality of Brussels sprouts (Brassica oleracea L. gemmifera DC) as affected by blanching method. J. Food Eng. 80, 218–225 (2007). https://doi.org/10.1016/j.jfoodeng.2006.02.049
H.-W. **ao, Z. Pan, L.-Z. Deng, H. El-Mashad, X.-H. Yang, A. Mujumdar, Z.-J. Gao, Q. Zhang, Recent developments and trends in thermal blanching: a comprehensive review. Inf. Process. Agric. 4, 101–127 (2017). https://doi.org/10.1016/j.inpa.2017.02.001
B. Ling, J. Tang, F. Kong, E.J. Mitcham, S. Wang, Kinetics of food quality changes during thermal processing: a review. Food Bioprocess. Technol. 8, 343–358 (2015). https://doi.org/10.1007/s11947-014-1398-3
S. Lee, Y. Choi, H.S. Jeong, J. Lee, J. Sung, Effect of different cooking methods on the content of vitamins and true retention in selected vegetables. Food Sci. Biotechnol. 27, 333–342 (2017). https://doi.org/10.1007/s10068-017-0281-1
M. Paciulli, T. Ganino, I.G.M. Meza, M. Rinaldi, M. Rodolfi, M. Morbarigazzi, E. Chiavaro, High pressure and thermal processing on the quality of zucchini slices. Eur. Food Res. Technol. 247, 475–484 (2021). https://doi.org/10.1007/s00217-020-03640-7
V. Rungapamestry, A. Duncan, Z. Fuller, B. Ratcliffe, Effect of cooking brassica vegetables on the subsequent hydrolysis and metabolic fate of glucosinolates. Proceed Nutr. Soc. 66, 69–81 (2007). https://doi.org/10.1017/S0029665107005319
A. Patras, B.K. Tiwari, N.P. Brunton, Influence of blanching and low temperature preservation strategies on antioxidant activity and phytochemical content of carrots, green beans and broccoli. LWT 44, 299–306 (2011). https://doi.org/10.1016/j.lwt.2010.06.019
F.I.G. Neves, C.L.M. Silva, M.C. Vieira, Combined pre-treatments effects on zucchini (Cucurbita pepo L.) squash microbial load reduction. Int. J. Microbiol. 305, 108257 (2019). https://doi.org/10.1016/j.ijfoodmicro.2019.108257
B. Liu, X. Fan, C. Shu, W. Zhang, W. Jiang, Comparison of non-contact blanching and traditional blanching pretreatment in improving the product quality, bioactive compounds, and antioxidant capacity of vacuum-dehydrated apricot. J. Food Proc. Pres. 43, 1–10 (2019). https://doi.org/10.1111/jfpp.13890
AOAC, Official Methods of Analysis, 18th edn. (Association of Official Analytical Chemists, Arlington, 2005)
M.J. Moyano, A.J. Melendez-Martiınez, J. Alba, F.J. Heredia, A comprehensive study on the color of virgin olive oils and its relationship with their chlorophylls and carotenoids indexes (II): CIELUV and CIELAB uniform color spaces. Food Res. Int. 41, 513–521 (2008). https://doi.org/10.1016/j.foodres.2008.03.007
M. McLellan, L. Lind, R. Kime, Hue angle determinations and statistical analysis for multiquadrant Hunter L, a, b Data. J. Food Qual. 18, 235–240 (2007). https://doi.org/10.1111/j.1745-4557.1995.tb00377.x
W. Mokrzycki, M. Tatol, Color difference Delta E: a survey. Mach. Gr. Vis. 20, 383–411 (2011)
M. Leporini, M.R. Loizzo, V. Sicari, T.M. Pellicanò, A. Reitano, A. Dugay, B. Deguin, R. Tundis, Citrus × Clementina Hort. juice enriched with its by-products (peels and leaves): chemical composition, in vitro bioactivity, and impact of processing. Antioxidants 9, 298–330 (2020). https://doi.org/10.3390/antiox9040298
I.M. de Carvalho Tavares, B. Rocchetti Sumere, S. Gómez Alonso, E. Gomes, I. Hermosín-Gutiérrez, R. Da-Silva, E. Silva Lago-Vanzela, Storage stability of the phenolic compounds, color and antioxidant activity of jambolan juice powder obtained by foam mat drying. Food Res. Int. 128, 108750 (2020). https://doi.org/10.1016/j.foodres.2019.108750
M. Leporini, R. Tundis, V. Sicari, T.M. Pellicanò, A. Dugay, B. Deguin, M.R. Loizzo, Impact of extraction processes on phytochemicals content and biological activity of Citrus × clementina Hort. Ex Tan. leaves: new opportunity for under-utilized food by-products. Food Res. Int. 127, 108742 (2020). https://doi.org/10.1016/j.foodres.2019.108742
M.R. Loizzo, R. Tundis, S. Sut, S. Dallacqua, V. Ilardi, M. Leporini, T. Falco, V. Sicari, M. Bruno, High-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MSn) analysis and bioactivity useful for prevention of “Diabesity” of Allium commutatum Guss. Plant Foods Hum. Nutr. 75, 124–130 (2020). https://doi.org/10.1007/s11130-019-00782-2
J. Coupland, N.B. Shaw, F. Monahan, E. O’Riordan, M. O’Sullivan, Modeling the effect of glycerol on the moisture sorption behavior of whey protein edible films. J. Food Eng. 43, 25–30 (2020). https://doi.org/10.1016/S0260-8774(99)00129-6
A.D.C. Mondragón-Portocarrero, B. Pena-Martínez, E. Fernández-Fernández, A. Romero-Rodríguez, L. Vázquez-Odériz, Effects of different pre-freezing blanching procedures on the physicochemical properties of Brassica rapa leaves (Turnip Greens, Grelos). Int. J. Food Sci. Technol. 41, 1067–1072 (2006). https://doi.org/10.1111/j.1365-2621.2006.01180.x
J.L. De Reyes-Corcuera, P.R. Cavalieri, J.R. Powers, Blanching of Foods-Encyclopedia of Agricultural, Food, and Biological Engineering (Marcell Dekker Inc, Madison, 2004), pp. 1–5
C. Quarcoo, F. Wireko-Manu, The effect of steam and hot water blanching on some quality attributes of cocoyam leaf. MOJ Food Process. Technol. 2, 1–5 (2016). https://doi.org/10.15406/mojfpt.2016.02.00050
M. Kaushal, K.D. Sharma, S. Attri, Effect of blanching on nutritional quality of dehydrated colocasia, Colocasia esculenta (L.) Schott leaves. Ind. J. Nat. Prod. Res. 4, 161–164 (2013)
S. Martinez, N. Perez, J. Carballo, I. Franco, Effect of blanching methods and frozen storageon some quality parameters of turnip greens (“grelos”). J. Food Sci. Technol. 51, 383–392 (2013). https://doi.org/10.1016/j.lwt.2012.09.020
M.N. Lewu, P.O. Adebola, A.J. Afolayan, Effect of cooking on the mineral and antinutrient content of the leaves of seven accessions of Colocasia esculenta (L.) Schott growing in South Africa. J. Food Agric. Environ. 7, 359–363 (2009)
A.M. Rocha, J.F. Ferreira, Â.M. Silva, G.N. Almeida, A.M. Morais, Quality of grated carrot (var. Nantes) packed under vacuum. J. Sci. Food Agric. 87, 447–451 (2007). https://doi.org/10.1002/jsfa.2723
S.K. Sra, K.S. Sandhu, P. Ahluwalia, Effect of treatments and packaging on the quality of dried carrot slices during storage. J. Food Sci. Technol. 51, 159–166 (2014). https://doi.org/10.1007/s13197-011-0575-x
M.N.A. Hawlader, C.O. Perera, M. Tian, Properties of modified atmosphere heat pump dried foods. J. Food Eng. 74, 392–401 (2006). https://doi.org/10.1016/j.jfoodeng.2005.03.028
V. Gnanasekharan, R.L. Shewfelt, M.S. Chinnan, Detection of color changes in green vegetables. J. Food Sci. 57, 149–154 (1992). https://doi.org/10.1111/j.1365-2621.1992.tb05444.x
C. Miglio, E. Chiavaro, A. Visconti, V. Fogliano, N. Pellegrini, Effects of different cooking methods on nutritional and physicochemical characteristics of selected vegetables. J. Agric. Food Chem. 56, 139–147 (2008). https://doi.org/10.1021/jf072304b
C.S. Saini, H.K. Sharma, Effect of pectin coating on color and quality of dehydrated pineapple during storage. Asian J. Dairy Food Res. 35, 120–129 (2016). https://doi.org/10.18805/ajdfr.v35i2.10721
I. Iswaldi, D. Arráez-Román, A.M.G. Caravaca, J. Lozano-Sánchez, A.A. Segura Carretero, A. Fernández-Gutiérrez, Profiling of phenolic and other polar compounds in zucchini (Cucurbita pepo L.) by reverse-phase high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. Food Res. Int. 50, 77–84 (2013). https://doi.org/10.1016/j.foodres.2012.09.030
M.A.A. Seleim, M.A.M. Hassan, A.S.M. Saleh, Changes in nutritional quality of zucchini (Cucurbita pepo L.) vegetables during the maturity. J. Food Dairy Prod. 6, 613–624 (2015). https://doi.org/10.21608/JFDS.2015.50079
G. Oboh, Effect of blanching on the antioxidant properties of some tropical green leafy vegetables. LWT 38, 513–517 (2005). https://doi.org/10.1016/j.lwt.2004.07.007
A.-N. Kim, K.-Y. Lee, M. Rahman, H.-J. Kim, J. Chun, H.J. Heo, W. Kerr, S.G. Choi, Effect of water blanching on phenolic compounds, antioxidant activities, enzyme inactivation, microbial reduction, and surface structure of samnamul (Aruncus dioicus var kamtschaticus). Int. J. Food Sci. Technol. 55, 1754–1762 (2020). https://doi.org/10.1111/ijfs.14424
L.K. Caesar, N.B. Cech, Synergy and antagonism in natural product extracts: when 1 + 1 does not equal 2. Nat. Prod. Rep. 36, 869–888 (2019). https://doi.org/10.1039/c9np00011a
R. Wang, W. Zhou, X. Jiang, Reaction kinetics of degradation and epimerization of epigallocatechin gallate (EGCG) in aqueous system over a wide temperature range. J. Agric. Food Chem. 56, 2694–2701 (2008). https://doi.org/10.1021/jf0730338
N. Li, L.S. Taylor, M.G. Ferruzzi, L.J. Mauer, Kinetic study of catechin stability: effects of pH, concentration and temperature. J. Agric. Food Chem. 60, 12531–12539 (2012). https://doi.org/10.1021/jf304116s
B. Moldovan, A. Popa, L. David, Effects of storage temperature on the total phenolic content of cornelian cherry (Cornus mas L.) fruits extracts. J. Appl. Bot. Food Qual. 89, 208–211 (2016). https://doi.org/10.5073/JABFQ.2016.089.026
E. Fernández-Romero, S.G. Chavez-Quintana, R. Siche, E.M. Castro-Alayo, F.P. Cardenas-toro, the kinetics of total phenolic content and monomeric Flavan-3-ols during the roasting process of criollo cocoa. Antioxidants 9, 146 (2020). https://doi.org/10.3390/antiox9020146
E. Nakilcioglu-Taş, S. Otleş, Degradation kinetics of bioactive compounds and antioxidant capacity of Brussels sprouts during microwave processing. Int. J. Food Prop. 20(S3), S2798–S2809 (2017). https://doi.org/10.1080/10942912.2017.1375944
K.S. Sonam, S. Guleria, Synergistic antioxidant activity of natural products. Ann. Pharmacol. Pharm. 2, 1086 (2017)
Y. Tanongkankit, N. Chiewchan, S. Devahastin, Evolution of antioxidants in dietary fiber powder produced from white cabbage outer leaves: effects of blanching and drying methods. Food Sci. Technol. 52, 2280–2287 (2015). https://doi.org/10.1007/s13197-013-1203-8
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VS: conceptualization; ML, RR and TMP: formal analysis; VS and RT: data curation, writing-original draft preparation; MRL: supervision, writing-review and editing. All authors have read and agreed to the published version of the manuscript. All authors discussed the results and contributed to the final manuscript.
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Sicari, V., Romeo, R., Leporini, M. et al. Comparison of traditional hot water and vacuum assisted blanching methods on the physico-chemical quality parameters and antioxidant activity of zucchini (Cucurbita pepo L.) slices. Food Measure 16, 281–294 (2022). https://doi.org/10.1007/s11694-021-01158-4
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DOI: https://doi.org/10.1007/s11694-021-01158-4