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Development and validation of an LC-HRMS method for the determination of pyrrolizidine alkaloids and quinolones in honey employing a simple alkaline sample dilution

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Abstract

Honey residue and contaminant assessments are extremely important, as they deal with food and therapeutic safety issues of a widely consumed animal product. Pyrrolizidine alkaloids are secondary plant metabolites used by bees in honey production. The toxicity of these alkaloids has been discussed in the literature and health risks have been noted, due to their occurrence in both honey and in pyrrolizidine alkaloid-producing plants. Quinolones comprise a class of antimicrobials associated with hypersensitivity reactions and bacterial resistance, one of the world's most significant public health problems. In this context, the aim of this study was to evaluate the simultaneous presence of pyrrolizidine alkaloids and quinolones in different honey types. A simple and novel analytical method employing liquid chromatography coupled to high-resolution mass spectrometry using a quadrupole-time-of-flight hybrid analyzer (Q-TOF) was developed and validated. No quinolone residues were detected in 80 honey samples, while pyrrolizidine alkaloids were found in 49% of the investigated samples.

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Acknowledgements

The authors thank the fiocruz Analytical Methods Technology Platform Network.

Funding

This work was carried out with the support of the Higher Education Personnel Improvement Coordination - Brazil (CAPES) - Financing Code 001.

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Authors and Affiliations

  1. Departamento de Métodos Analíticos, Fundação Oswaldo Cruz, Farmanguinhos, Rio de Janeiro, Brazil

    Thiago Bousquet Bandini

  2. Laboratório de Resíduos de Medicamentos Veterinários Em Alimentos, Fundação Oswaldo Cruz, Instituto Nacional de Controle de Qualidade Em Saúde, Rio de Janeiro, Brazil

    Bernardete Ferraz Spisso

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  1. Thiago Bousquet Bandini
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Correspondence to Thiago Bousquet Bandini.

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Bandini, T.B., Spisso, B.F. Development and validation of an LC-HRMS method for the determination of pyrrolizidine alkaloids and quinolones in honey employing a simple alkaline sample dilution. Food Measure 15, 4758–4770 (2021). https://doi.org/10.1007/s11694-021-01048-9

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