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Label-free SERS detection of Salmonella Typhimurium on DNA aptamer modified AgNR substrates

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Abstract

A straightforward label-free method based on aptamer binding and surface enhanced Raman spectroscopy (SERS) has been developed for the detection of Salmonella Typhimurium, an important foodborne pathogen which causes gastroenteritis in both humans and animals. Surface of the SERS-active silver nanorod array substrates was modified with anti-S. Typhimurium DNA aptamer and mercaptohexanol, and reacted with S. Typhimurium and S. Enteritidis, E. coli, and E. faecalis as negative control bacteria. Some noticeable spectral changes, including a significantly higher ratio of the 725 and 680 cm−1 peak intensities (I 725/I 680) was found in the SERS spectra of S. Typhimurium compared to the negative and blank controls. Such spectral changes were confirmed by principle component analysis, and can potentially be used for specific detection of S. Typhimurium. Some limitations of this label-free SERS method with aptamers are also discussed.

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Acknowledgements

This work is funded by National Science Foundation under contract number Grant CBET-1064228 and Animal and Plant Quarantine Agency (APQA) Grant I-AD15-2010-13-1. The authors would like to thank Dr. Arthur Hilton, Jr. for providing the bacterial strains and Dr. Nasreen Bano for bacterial sample preparation.

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Authors and Affiliations

  1. Department of Food Science and Technology, University of Georgia, Athens, GA, 30602, USA

    **g Chen, Bosoon Park & Yao-wen Huang

  2. Department of Physics and Astronomy, University of Georgia, Athens, GA, 30602, USA

    Yi** Zhao

  3. U.S. National Poultry Research Center, Agricultural Research Services, United States Department of Agriculture, Athens, GA, 30605, USA

    **g Chen & Bosoon Park

  4. Nanoscale Science and Engineering Center, University of Georgia, Athens, GA, 30605, USA

    **g Chen, Yao-wen Huang & Yi** Zhao

  5. Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea

    Yongkuk Kwon

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  1. **g Chen
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  2. Bosoon Park
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  4. Yi** Zhao
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  5. Yongkuk Kwon
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Correspondence to Bosoon Park.

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Chen, J., Park, B., Huang, Yw. et al. Label-free SERS detection of Salmonella Typhimurium on DNA aptamer modified AgNR substrates. Food Measure 11, 1773–1779 (2017). https://doi.org/10.1007/s11694-017-9558-6

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  • DOI: https://doi.org/10.1007/s11694-017-9558-6

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