Abstract
A biosurfactant (BS) is a surface-active metabolite that is secreted by microbial metabolism, and can be used as a substitute for chemically synthesized surfactants. The first and most critical step to the successful application of BSs is to isolate bacterial strains with strong BS-producing capabilities. In this study, a BS-producing Serratia marcescens ZCF25 was isolated from the sludge of an oil tanker. Through polyphasic characterization using Fourier-transform infrared spectroscopy, thin layer chromatography, and gas chromatography-mass spectrometry, the produced BS was classified as a lipopeptide; it can decrease the water surface tension from 72.0 to 29.50 mN m−1 and has a critical micelle concentration of 220 mg/L. The BS showed a high tolerance over a wide range of pH (2–12), temperature (50–100 °C), and salinity (10–100 g/L). Furthermore, the inoculation of S. marcescens ZCF25 with fracturing flowback fluids could significantly (P < 0.05) reduce the chemical oxygen demand, concentration of alkanes, and concentration of polycyclic aromatic hydrocarbons, with removal efficiencies of 48.9%, 65.57%, and 64%, respectively. This is the first study on the application of BS-producing S. marcescens to treat fracturing flowback fluids. S. marcescens ZCF25 is a promising candidate for use in various industrial and bioremediation applications.
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Funding
This work was supported by the **nJiang Keli New Technology Development Co., Ltd. (K18-529102-014, K17-529102-004), the National Natural Science Foundation of China (No. 51978189), and the Research Funds of the Guangxi Key Laboratory of Theory and Technology for Environmental Pollution Control (No.1801 K011).
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Huang, Y., Zhou, H., Zheng, G. et al. Isolation and characterization of biosurfactant-producing Serratia marcescens ZCF25 from oil sludge and application to bioremediation. Environ Sci Pollut Res 27, 27762–27772 (2020). https://doi.org/10.1007/s11356-020-09006-6
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DOI: https://doi.org/10.1007/s11356-020-09006-6