Abstract
The acquisition of embryogenic competence involves aspects at a cellular level related to cell communication and signaling. For Araucaria angustifolia, transcriptome and proteome databases have indicated differential gene expression and protein abundance related to cell-to-cell communication during embryogenesis. The differences are mainly related to vesicular transport and regulation of plasmodesmata region-related proteins. This study aims to understand the behavior of cell-to-cell communication in cell cultures with contrasting embryogenic potential. We used two embryogenic cell lines of A. angustifolia (Responsive and Blocked) and carried out gene expression, coexpression network, immunocytochemical, and ultrastructural analyses. Eight proteins related to vesicular transport and cell-to-cell communication processes were identified with a significantly different abundance in both cell lines. One callose hydrolase (AaGHL17) was differentially expressed between the two cell lines. Accordingly, we used immunocytochemistry and found a higher intensity of callose in the Responsive, as opposed to the Blocked embryogenic cells. Transmission Electron Microscopy images highlighted a more intense vesicular activity in the Responsive cell line compared to the Blocked one. Different status of cell-to-cell intercommunication and vesicular transport is associated with somatic embryogenic competence.
Key message
Vesicular transport and plasmodesmata regulation features are associated with Araucaria angustifolia somatic embryogenic responsiveness.
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Acknowledgements
We thank Waldir Caldeira (University of São Paulo), Irwandro Pires (University of São Paulo), and Piero Bagnasi (Leica Microsystems) for support with the anatomical analyses. The research was supported by the São Paulo Research Foundation (FAPESP) (2014/26888-0 and 2016/17541-2), the Coordination for the Improvement of Higher Education Personnel (CAPES) (2013-88881.068434), the National Council of Technological and Scientific Development (CNPq) (307238/2013-0), and the National Institute of Science and Technology of Bioethanol (2014/50884-5).
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BN, AS, DD, MB, and EF conceived the experimental work; BN, PE, LO, AP, and DS carried out the experiments; BN, PE, and AP collected, analyzed, and interpreted the data. BN wrote the initial draft. All authors contributed to editing and the finalization of the manuscript.
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Navarro, B.V., Elbl, P., de Oliveira, L.F. et al. Cell-to-cell trafficking patterns in cell lines of Araucaria angustifolia (Brazilian pine) with contrasting embryogenic potential. Plant Cell Tiss Organ Cult 148, 81–93 (2022). https://doi.org/10.1007/s11240-021-02166-4
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DOI: https://doi.org/10.1007/s11240-021-02166-4