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Dual Fluorescence-colorimetric Silver Nanoparticles Based Sensor for Determination of Olanzapine: Analysis in Rat Plasma and Pharmaceuticals

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Abstract

The present work describes a dual-readout assay for the determination of an antipsychotic drug olanzapine using Rhodamine B modified silver nanoparticles (AgNPs). AgNPs, when mixed with Rhodamine B, quenched its fluorescence emission with high quenching efficiency as evident from the Stern Volmer plot. Transmission electron microscopy image and Dynamic Light Scattering histogram of Rhodamine B bound AgNPs showed a stable monodispersed nanosuspension. Addition of olanzapine to Rhodamine B-bound AgNPs resulted in reappearance of fluorescence, which was dependent on the amount of olanzapine added to the system. Besides displacing the surface bound Rhodamine B molecules, it caused aggregation of AgNPs which formed the basis of dual-readout sensor. Several parameters such as pH, reaction time and order of addition of the three components which may influence the analytical signal were studied and optimized. The method was validated for linearity, sensitivity, selectivity, accuracy, precision and recovery. Based on this dual-readout system, linear concentration range was established from 0.05 to 10 µM (fluorescence measurement) and 5.0 to 50 µM (colorimetric response) for olanzapine. The limit of detection (LOD) using fluorescence and colorimetric approach was 0.013 µM and 1.25 µM, respectively. The proposed method showed excellent selectivity for olanzapine in presence of several antipsychotic drugs, cations, sugars and amino acids. Finally, the method was successfully applied to a pharmacokinetic study of olanzapine in rats and also for analyzing pharmaceutical formulations.

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Acknowledgements

The authors thank Department of Chemistry, Gujarat University for supporting this work and Department of Zoology, Gujarat University for pharmacokinetic study.

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This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Chemistry, School of Sciences, Gujarat University, Ahmedabad, 380009, Gujarat, India

    Vijay D. Chavada, Nejal M. Bhatt & Pranav S. Shrivastav

  2. Department of Chemistry, St. Xavier’s College, Navrangpura, Ahmedabad, 380009, Gujarat, India

    Mallika Sanyal

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Contributions

The manuscript was written through contributions of all authors. VDC and NMB did the formal analysis, investigation, writing - original draft and data Curation. MS designed the methodology and involved with project administration, generation of resources and software. PSS contributed towards conceptualization, supervision, writing - review & editing. All authors read and approved the final manuscript.

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Correspondence to Pranav S. Shrivastav.

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All procedures performed in studies involving animals were in accordance with the ethical standards of the institutional review board and/or national research committee, and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. All the experimental protocols were approved by the Committee for the Purpose of Control and Supervision of Experiment on Animals (Reg. – 167/1999/CPCSEA), New Delhi, India

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Chavada, V.D., Bhatt, N.M., Sanyal, M. et al. Dual Fluorescence-colorimetric Silver Nanoparticles Based Sensor for Determination of Olanzapine: Analysis in Rat Plasma and Pharmaceuticals. J Fluoresc 30, 955–967 (2020). https://doi.org/10.1007/s10895-020-02568-1

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