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Applicability of Silver Nanoparticles as a Fluorescent Precursor for the Determination of Spectinomycin Hydrochloride in Human Plasma

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Abstract

A novel, rapid, and accurate ultra-sensitive spectrofluorimetric method was developed and applied for the determination of spectinomycin hydrochloride (SPEC) in real human plasma and pharmaceutical dosage form. Silver nanoparticles (AgNPs) fluorescence was measured at 415 nm (λex at 340 nm). The method was used for SPEC quantification in the concentration range of 0.7–30 ng/mL with the limit of quantitation equal to 0.5 ng/mL and the limit of detection of 0.19 ng/mL. The fluorescence of AgNPs was quenched via a gradual increase in SPEC concentration. The developed method was validated using ICH guidelines and bio-analytically validated using US-FDA recommendations. The simplicity and high sensitivity of the proposed method make it suitable to monitor SPEC to conduct an extensive pharmacokinetic study for SPEC in human plasma. Moreover, SPEC stability was studied in real human plasma. SPEC was also subjected to acidic, basic, oxidative, and photolytic forced degradation conditions according to ICH guidelines. As observed, SPEC showed extensive degradation under acid, base, and oxidative hydrolysis stress conditions.

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Authors and Affiliations

  1. Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Assiut branch, 71524, Assiut, Egypt

    Baher I. Salman, Walid E. Eltoukhi & Yasser F. Hassan

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  1. Baher I. Salman
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  2. Walid E. Eltoukhi
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  3. Yasser F. Hassan
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Correspondence to Baher I. Salman.

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Salman, B., Eltoukhi, W.E. & Hassan, Y.F. Applicability of Silver Nanoparticles as a Fluorescent Precursor for the Determination of Spectinomycin Hydrochloride in Human Plasma. J Anal Chem 77, 1440–1447 (2022). https://doi.org/10.1134/S1061934822110107

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