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Japanese Encephalitis Virus Envelope Protein Mitigates TNF-α mRNA Expression in RAW264.7 Cells

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Abstract

Japanese encephalitis virus (JEV) is known as an important mosquito-borne human pathogen that causes Japanese encephalitis and may lead to lethal effect. Since monocyte has been demonstrated to play transmissible role for JEV, rare study is reported to clarify the effect of JEV envelope (JEVE) protein on monocyte. This study intends to investigate the effects of JEVE protein inside monocyte. Notably, significant decreased expression of tumour necrosis factor (TNF)-α mRNA in RAW264.7 cells transfected with pEGFP-JEVE was observed as compared to those cells transfected with pEGFP. Increased p21Waf1/Cip1 protein was observed in both pEGFP and pEGFP-JEVE transfected RAW264.7 cells. However, increased p53 protein was only detected in pEGFP-transfected cells but not pEGFP-JEVE transfected cells as well as the result that no increased expression of nuclear factor-kB was observed in pEGFP-JEVE transfected cells. These experimental results indicate the effects of JEVE protein in alleviating TNF-α mRNA expression that is associated with the increased p53-independent p21Waf1/Cip1 expression and provide an explanation in the role of JEV transmission through monocyte.

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Abbreviations

JEV:

Japanese encephalitis virus

TNF:

tumour necrosis factor

NF-kB:

nuclear factor-kB

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Acknowledgements

This study was supported by the grant CSMU 95-OM-B-030 from Chung Shan Medical University, Taichung, Taiwan, R.O.C.

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Authors and Affiliations

  1. Institute of Immunology, Chung Shan Medical University, Taichung, Taiwan, Republic of China

    Tsai-Ching Hsu

  2. Institute of Biochemistry and Biotechnology, Chung Shan Medical University, 110 section 1, Jianguo N. Road, Taichung, 402, Taiwan, Republic of China

    Ji-Qiang Gao & Bor-Show Tzang

  3. School of Medicine, Chung Shan Medical University, Taichung, Taiwan, Republic of China

    Ko-Hsiu Lu

  4. Department of Orthopaedic Surgery, Chung Shan Medical University Hospital, Taichung, Taiwan, Republic of China

    Ko-Hsiu Lu

  5. Department of Healthcare Administration, Asia University, Taichung, Taiwan, Republic of China

    Chang-Hai Tsai

  6. Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan, Republic of China

    Chih-Yang Huang

  7. Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, Republic of China

    Chih-Yang Huang

  8. Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan, Republic of China

    Chih-Yang Huang

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  1. Tsai-Ching Hsu
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  2. Ji-Qiang Gao
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  4. Chang-Hai Tsai
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Corresponding author

Correspondence to Bor-Show Tzang.

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Huang and Tzang share equal contributions.

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Hsu, TC., Gao, JQ., Lu, KH. et al. Japanese Encephalitis Virus Envelope Protein Mitigates TNF-α mRNA Expression in RAW264.7 Cells. Inflammation 31, 133–140 (2008). https://doi.org/10.1007/s10753-008-9058-2

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  • DOI: https://doi.org/10.1007/s10753-008-9058-2

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