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Characterization and temporal–spatial expression analysis of LEC1 gene in the development of seedless berries in grape induced by gibberellin

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Abstract

Grape (Vitis vinifera L.), a commercial fruit crop, is profoundly sensitive to gibberellic acid (GA), however exogenous application can significantly induce seedless grape berries. Nevertheless, the underlying molecular mechanism of grape leafy cotyledon 1 (LEC1) on seedless induction remains intangible. Herein, the morphological changes of ‘Wink’ grape cultivar during berry development in response to GA treatments were recorded. To better comprehend the function of VvLEC1 in grape seedless berry induced by GA, the gene and protein sequence were determined by bioinformatics methods, of which motif elements analysis intimates the potential function in seed development. The expression level of VvLEC1 which was repressed by GA treatment, determined by RT PCR, exhibited highest up-regulation at four weeks after flowering in the seed. Furthermore, VvLEC1, a member of the nuclear factor Y subunit B (NF-YB-type) transcription factor, interacts with five proteins which belong to the NF-YC-type transcription factor by the prediction of protein interaction network, thus indicating their collective effects on the development of grape seed. Our research findings elucidate a novel molecular insight into the role of VvLEC1 responsive to GA in accentuating grapevine seed development, which is crucial for the molecular breeding of high-quality seedless grapes.

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Abbreviations

LEC1:

Leafy cotyledon 1

GA:

Gibberellin acid

NF-YC:

Nuclear factor Y subunit C

qRT-PCR:

Quantitative real time polymerase chain reaction

WAF:

Weeks after full bloom

cDNA:

Coding DNA

ORF:

Open reading frame

SA:

Salicylic acid

ABA:

Abscisic acid

CBF:

CCAAT binding factor

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Acknowledgements

This work was supported by a grant from the Central University basic scientific research business fee independent innovation major project (NATURAL SCIENCE) (KYTZ201602), and Key Laboratory of Fruit Breeding Technology, Ministry of Agriculture of China, Zhengzhou, Henan, 450009, P.R. China.

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Authors and Affiliations

  1. Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, 450000, China

    Mengjie Cui, **ucai Fan & Chonghuai Liu

  2. College of Horticulture, Nan**g Agricultural University, Weigang No.1, Nan**g, 210095, China

    Mengjie Cui, Wenran Wang, Le Guan, Ting Zheng, Xudong Zhu, Haifeng Jia, **ggui Fang & Chen Wang

  3. College of Agriculture, Nan**g Agricultural University, Nan**g, 210095, China

    Mengjie Cui

  4. School of Life Sciences, Nan**g University, Nan**g, 210023, China

    Fengfei Guo

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  1. Mengjie Cui
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Contributions

CW and CL conceived the study and carried out the molecular mechanism analysis. MC and WW cloned and identify the precise sequence of VvLEC1. MC, FG, XF and LG conducted the expression analysis of VvLEC1 in grape berry development stages. TZ, XZ and HJ performed the promoter element analysis of VvLEC1. WW and CW preliminarily validate the activity of VvLEC1 promoters in response to GA. JF predict the interacting proteins of VvLEC1. MC drew the tables and figures. MC and CW drafted the manuscript. CW, JF and CL revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Chen Wang or Chonghuai Liu.

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Mengjie Cui and Wenran Wang are co-first authors.

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Cui, M., Wang, W., Guo, F. et al. Characterization and temporal–spatial expression analysis of LEC1 gene in the development of seedless berries in grape induced by gibberellin. Plant Growth Regul 90, 585–596 (2020). https://doi.org/10.1007/s10725-020-00582-8

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