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LCVD resistance breeding in heat tolerant bell pepper: Combined phenotypic and marker-assisted backcrossing for introgression of LCVD resistance from hot pepper

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Abstract

Hot pepper (Capsicum annuum var. annuum) and bell pepper (C. annuum var. grossum) are the important crops of the Solanaceae family. In the present study, marker-assisted selection (MAS) was employed for interspecific leaf curl virus disease (LCVD) resistant gene introgression from hot pepper (S-343) to heat-tolerant bell pepper lines. Segregating populations were developed using resistant (S-343) and susceptible (bell pepper) genotypes for understanding the genetics of LCVD resistance. The micro-cage artificial screening technique was used for the phenotypic selection of LCVD resistant plants from progenies of each population. The F1 of all crosses were resistant to LCVD, while F2 and BC1F2 generations of all the crosses were segregated into 3:1 (resistant:susceptible) and BC1F1 generations of all crosses were a good fit to ratio 1:1 (resistant:susceptible) suggesting monogenic dominant control of LCVD resistance. In addition to phenotypic selection for LCVD resistance, MAS for non-pungency was done using ‘BF7’ and ‘BR9’-SCAR primers along with the very stringent phenoty** (background selection) in backcrossed populations for most important fruit traits. The background selection was performed for the recovery of recurrent parent phenome and it was measured as shift in means of these important fruit traits. To the best of our knowledge, it is a novel experiment of introgression of LCVD resistance in heat tolerant bell pepper lines that led to the successful development of six improved LCVD resistant, heat-tolerant and non-pungent BC2F1 populations (LCVRS3SM-1, LCVRS3SM-2, LCVRS3SM-8, LCVRS3SM-4, LCVRS3SM-17, and LCVRS3SM-58) that will help in future bell pepper resistant breeding programs.

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References

  • Ahmed F, Rafii MY, Ismail MR, Juraimi AS, Rahim HA, Tanweer FA, Latif MA (2016) Recurrent parent genome recovery in different populations with the introgression of Sub1 gene from a cross between MR219 and Swarna-Sub1. Euphytica 207:605–618. https://doi.org/10.1007/s10681-015-1554-5

    Article  Google Scholar 

  • Anandhi K, Khader KM (2011) Gene effects of fruit yield and leaf curl virus resistance in interspecific crosses of chilli (C. annuum L. and C. frutescens L.). J Trop Agric 49:107–109

    Google Scholar 

  • Bal SS, Singh J, Dhanju KC (1995) Genetics of resistance to mosaic and leaf curl viruses in chilli (Capsicum annuum L.). Indian J Virol 11:77–79

    Google Scholar 

  • Barboza GE, Agra MF, Romero MV, Scaldaferro MA, Moscone EA (2011) New endemic species of Capsicum (Solanaceae) from the Brazilian Caatinga: comparison with the recircumscribed C. parvifolium. Syst Bot 36:768–781. https://doi.org/10.1600/036364411X583718

    Article  Google Scholar 

  • Basavaraj SH, Singh VK, Singh A, Singh A, Singh A, Anand D, Yadav S, Ellur RK, Singh D, Krishnan SG, Nagarajan M, Mohapatra T, Prabhu KV, Singh AK (2010) Marker-assisted improvement of bacterial blight resistance in parental lines of Pusa RH10, a superfine grain aromatic rice hybrid. Mol Breed 26:293–305. https://doi.org/10.1007/s11032-010-9407-3

    Article  CAS  Google Scholar 

  • Bosland PW (1992) Chiles: a diverse crop. Hort Technol 2:6–10

    Article  Google Scholar 

  • Cervantes PB, Yahia EM, Ornelas PJ, Victoria CI, Junquera V, Martínez JD, Minakata P (2014) Antioxidant activity and content of chlorophylls and carotenoids in raw and heat-processed Jalapeño peppers at intermediate stages of ripening. Food Chem 146:188–196. https://doi.org/10.1016/j.foodchem.2013.09.060

  • da Costa Batista FR (2016) Cytogenetics in Capsicum L. In: do Rêgo ER, do Rêgo MM, Finger FL (eds) Production and breeding of chilli peppers (Capsicum spp). Springer International Publishing, Switzerland, pp 41–56. https://doi.org/10.1007/978-3-319-06532-8_3

    Chapter  Google Scholar 

  • Dhaliwal MS, Sharma A (2016) Breeding for resistance to virus diseases in vegetable crops. In: Peter KV (ed) Innovations in horticultural science. New India Publishing Agency, New Delhi, pp 303–327

    Google Scholar 

  • Dhanraj KS, Seth ML (1968) Enations in Capsicum annuum L. (chilli) caused by a new strain of leaf curl virus. Indian J Hort 25:70–71

    Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Gopalakrishnan S, Sharma RK, Rajkumar KA, Joseph M, Singh VP, Singh AK, Bhat KV, Singh NK, Mohapatra T (2008) Integrating marker assisted background analysis with foreground selection for identification of superior bacterial blight resistant recombinants in Basmati rice. Plant Breed 127:131–139. https://doi.org/10.1111/j.1439-0523.2007.01458.x

    Article  CAS  Google Scholar 

  • Grubben GJH, El-Tahir IM (2004) Capsicum annuum L. In: Grubben GJH, Denton OA (eds) PROTA 2: Vegetables/Légumes.+ [CDRom] plant resouirces of tropical Africa. Wageningen. Backhuys Publishers, Leiden

    Google Scholar 

  • Herath MSN, Rafii MY, Ismail SI, Nakasha JJ, Ramlee SI (2020) Improvement of important economic traits in chilli through heterosis breeding: a review. J Horticul Sci Biotech. https://doi.org/10.1080/14620316.2020.1780162

    Article  Google Scholar 

  • Hernandez-Verdugo S, Guevara-Gonzalez RG, Rivera-Bustamante RF, Oyama K (2001) Screening wild plants of Capsicum annuum for resistance to pepper huasteco virus (PHV): presence of viral DNA and differentiation among populations. Euphytica 122:31–36. https://doi.org/10.1023/A:1012624830340

    Article  CAS  Google Scholar 

  • Jeong HS, Jang S, Han K, Kwon JK, Kang BC (2015) Marker-assisted backcross breeding for development of pepper varieties (Capsicum annuum) containing capsaicinoids. Mol Breed 35:226. https://doi.org/10.1007/s11032-015-0417-z

    Article  CAS  Google Scholar 

  • **dal SK, Dhaliwal MS (2019) PSM-1: a high yielding bell pepper variety having seed production potential under North Indian plains. Veg Sci 46:123–125

    Google Scholar 

  • **dal SK, Dhaliwal MS, Sharma A, Thakur H (2018) Inheritance studies for resistance to leaf curl virus disease in chilli (Capsicum annuum L.). Agric Res J 55:757–760. https://doi.org/10.5958/2395-146X.2018.00139.4

    Article  Google Scholar 

  • Joseph M, Gopalakrishnan S, Sharma RK, Singh VP, Singh AK, Singh NK, Mohapatra T (2004) Combining bacterial blight resistance and Basmati quality characteristics by phenotypic and molecular marker-assisted selection in rice. Mol Breed 13:377–387. https://doi.org/10.1023/B:MOLB.0000034093.63593.4c

    Article  CAS  Google Scholar 

  • Kumar S, Kumar R, Kumar S, Singh M, Rai AB, Rai M (2009) Reaction of Pepper leaf curl virus field resistance of chilli (C. annuum L.) genotypes under challenged condition. Veg Sci 36:230–232

    Google Scholar 

  • Kumar S, Kumar R, Kumar S, Singh AK, Singh M, Rai AB, Rai M (2011) Incidence of leaf curl disease on Capsicum germplasm under field conditions. Indian J Agric Sci 81:187–189

    Google Scholar 

  • Lee CJ, Yoo EY, Shin JH, Lee J, Hwang HS, Kim BD (2005) Non-pungent Capsicum contains a deletion in the capsaicinoid synthetase gene, which allows early detection of pungency with SCAR markers. Mol Cells 19:262–267

    CAS  PubMed  Google Scholar 

  • Lin SW, Shieh HC, Wang YW, Tan CW, Schafleitner R, Yang WJ, Kumar S (2015) Restorer breeding in sweet pepper: introgressing Rf allele from hot pepper through marker-assisted backcrossing. Sci Hortic 197:170–175. https://doi.org/10.1016/j.scienta.2015.09.036

    Article  CAS  Google Scholar 

  • Lindsay EW, Nancy TE, Giulia MS, Michael M (2012) Development and application of a suite of non-pungency markers for the Pun1 gene in pepper (Capsicum spp.). Mol Breed 30:1525–1529. https://doi.org/10.1007/s11032-012-9716-9

    Article  Google Scholar 

  • Mckinney HH (1923) Influence of soil temperature and moisture on infection of wheat seedling by Helminthosporium sativum. J Agric Res 26:195–217

    Google Scholar 

  • Mendes N, Branco A, Gonc EC (2020) The role of bioactive components found in peppers. Trends Food Sci Technol 99:229–243. https://doi.org/10.1016/j.tifs.2020.02.032

  • Miah G, Rafii MY, Ismail MR, Puteh AB, Rahim HA, Latif MA (2015) Recurrent parent genome recovery analysis in a marker-assisted backcrossing program of rice (Oryza sativa L.). C R Biol 338:83–94. https://doi.org/10.1016/j.crvi.2014.11.003

    Article  PubMed  Google Scholar 

  • Pagamas P, Nawata E (2008) Sensitive stages of fruit and seed development of chilli pepper (Capsicum annuum L. var. Shishito) exposed to high-temperature stress. Sci Hortic 117:21–25. https://doi.org/10.1016/j.scienta.2008.03.017

    Article  CAS  Google Scholar 

  • Pressman E, Moshkovitch H, Rosenfeld K, Shaked R, Gamliel B, Aloni B (1998) Influence of low night temperatures on sweet pepper flower quality and the effect of repeated pollinations, with viable pollen, on fruit setting. J Hort Sci Biotechnol 73:131–136. https://doi.org/10.1080/14620316.1998.11510955

    Article  Google Scholar 

  • Qin C, Yu C, Shen Y, Fang X, Chen L, Min J, Cheng J, Zhao S, Xu M, Luo Y, Yang Y (2014) Whole-genome sequencing of cultivated and wild peppers provides insights into Capsicum domestication and specialization. Proc Natl Acad Sci 111:5135–5140. https://doi.org/10.1073/pnas.140097511

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rai VP, Kumar R, Pratap S, Kumar S, Kumar S, Singh M, Rai M (2014) Horticulturae monogenic recessive resistance to pepper leaf curl virus in an interspecific cross of capsicum. Sci Hortic 172:34–38. https://doi.org/10.1016/j.scienta.2014.03.039

    Article  Google Scholar 

  • Rani M, **dal SK, Vikal Y, Meena OP (2021) Genetic male sterility breeding in heat tolerant bell pepper: introgression of ms10 gene from hot pepper through marker-assisted backcrossing. Sci Hortic. https://doi.org/10.1016/j.scienta.2021.110172

    Article  Google Scholar 

  • Senanayake DMJB, Mandal B, Lodha S, Varma A (2007) First report of Chilli leaf curl virus affecting chilli in India. Plant Pathol 56:343. https://doi.org/10.1111/j.1365-3059.2007.01513.x

    Article  Google Scholar 

  • Senanayake DMJB, Varma A, Mandal BJ (2012) Virus–vector relationships, host range, detection and sequence comparison of chilli leaf curl virus associated with an epidemic of leaf curl disease of chilli in Jodhpur, India. J Phytopathol 160:146–155. https://doi.org/10.1111/j.1439-0434.2011.01876.x

    Article  CAS  Google Scholar 

  • Sharma A, **dal SK, Thakur H (2018) Phenotypic classes of leaf curl virus disease severity for nursery screening in chilli pepper. Pl Dis Res 33:99–103

    Google Scholar 

  • Singh VK, Singh A, Singh SP, Ellur RK, Singh D, Krishnan SG, Bhowmick PK, Nagarajan M, Vinod KK, Singh UD, Mohapatra T, Prabhu KV, Singh AK (2013) Marker-assisted simultaneous but stepwise backcross breeding for pyramiding blast resistance genes Piz5 and Pi54 into an elite Basmati rice restorer line “PRR78.” Plant Breed 132:486–495. https://doi.org/10.1111/pbr.12077

    Article  CAS  Google Scholar 

  • Sran TS, **dal SK, Sharma A, Chawla N (2023) Genetics of novel leaf curl virus disease resistant pepper genotypes and antioxidative profile analysis of their progenies. Sci Hortic. https://doi.org/10.1016/j.scienta.2022.111563

    Article  Google Scholar 

  • Tanaka Y, Yoneda H, Hosokawa M, Miwa T, Yazawa S (2014) Application of marker assisted selection in breeding of a new fresh pepper cultivar (Capsicum annuum) containing capsaicinoids, low-pungent capsaicinoid analogs. Sci Hortic 165:242–245. https://doi.org/10.1016/j.scienta.2013.10.025

    Article  CAS  Google Scholar 

  • Thakur H, **dal SK, Sharma A, Dhaliwal MS (2018) Chilli leaf curl virus disease: a serious threat for chilli cultivation. J Plant Dis Prot. https://doi.org/10.1007/s41348-018-0146-8

    Article  Google Scholar 

  • Thakur H, **dal SK, Sharma A, Dhaliwal MS (2019) A monogenic dominant resistance for leaf curl virus disease in chilli pepper (Capsicum annuum L.). Crop Prot 116:115–120. https://doi.org/10.1016/j.cropro.2018.10.007

    Article  Google Scholar 

  • Thakur H (2018) Genetics and molecular map** of leaf curl virus disease resistance gene(s) in chilli pepper (Capsicum annuum L.). Ph.D. dissertation. Punjab Agricultural University, Ludhiana

  • Usman MG, Rafii MY, Martini MY, Yusuff OA, Ismail MR, Miah G (2018) Introgression of heat shock protein (Hsp70 and sHsp) genes into the Malaysian elite chilli variety Kulai (Capsicum annuum L.) through the application of marker-assisted backcrossing (MAB). Cell Stress Chapero 23:223–234. https://doi.org/10.1007/s12192-017-0836-3

    Article  CAS  Google Scholar 

  • Wang D, Bosland PW (2006) The genes of Capsicum. Hort Sci 41:1169–1187. https://doi.org/10.21273/HORTSCI.41.5.1169

    Article  CAS  Google Scholar 

  • Wyatt SD, Brown JK (1996) Detection of subgroup III geminivirus isolates in leaf extracts by degenerate primers and polymerase chain reaction. Phytopathol 86:1288–1293

    Article  Google Scholar 

  • Zhang Z, Zhu Y, Cao Y, Yu H, Bai R, Zhao H, Zhang B, Wang L (2020) Fine map** of the male fertility restoration gene CaRf032 in Capsicum annuum L. Theor Appl Genet 133:1177–1187. https://doi.org/10.1007/s00122-020-03540-0

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

Authors are thankful to Confederation of Indian Industry, Department of Science and Technology and Arizona Seeds Pvt. Ltd. for providing Prime Minister's Fellowship to Tejpal Singh Sran

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Authors and Affiliations

  1. Department of Vegetable Science, Punjab Agricultural University, Ludhiana, 141004, India

    Tejpal Singh Sran, Salesh Kumar **dal & Abhishek Sharma

  2. Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, 141004, India

    Dharminder Bhatia

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  1. Tejpal Singh Sran
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Contributions

SKJ, AS and DB: Conceptualization, methodology, visualization, supervision. TSS: Investigation, Software, data curation, writing—original draft preparation, writing—reviewing and editing. SKJ, TSS, AS and DB: Validation and reviewing.

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Correspondence to Tejpal Singh Sran.

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Sran, T.S., **dal, S.K., Sharma, A. et al. LCVD resistance breeding in heat tolerant bell pepper: Combined phenotypic and marker-assisted backcrossing for introgression of LCVD resistance from hot pepper. Euphytica 219, 50 (2023). https://doi.org/10.1007/s10681-023-03182-5

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