SpringerLink
Log in

Introgression of multiple disease resistance into a maintainer of Basmati rice CMS line by marker assisted backcross breeding

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Globally, blast incited by Magnaporthe oryzae and sheath blight (ShB) by Rhizoctonia solani Kuhn forms two major fungal diseases that cause significant yield loss in rice. Pusa 6B, the Basmati quality maintainer line of the popular superfine grain aromatic rice hybrid Pusa RH10, is highly susceptible to both the diseases. The rice cultivar Tetep was used as the donor for transferring a major blast resistant gene, Pi54 and three ShB resistant quantitative trait loci (QTLs) namely, qSBR11-1, qSBR11-2 and qSBR7-1 into Pusa 6B using a marker assisted backcross breeding scheme with restricted number of backcrosses. Plants heterozygous for the alleles of interest and phenotypic similarity to the recurrent parent were used for generating BC1F2 population by selfing selected BC1F1 plants. Selected BC1F2 plants homozygous for Pi54 were selfed to generate BC1F3 families that were subjected to a step-wise reductive screening for the three ShB resistant QTLs. Final selections were advanced to BC1F5 generation through selfing while subjecting to stringent phenotypic selection. The advanced selections carrying blast and ShB resistant genes (Pi54, qSBR11-1, qSBR11-2, qSBR7-1) in the background of Pusa 6B were resistant to highly virulent strains of rice blast as well as ShB isolates without compromising the grain and cooking quality of Pusa 6B. Marker assisted transfer of blast and ShB resistance into Pusa 6B will aid in develo** CMS lines with inbuilt resistance to these diseases. When combined with restorer lines possessing resistant genes/QTLs for these diseases, the improved Pusa6A lines will aid in development of improved Pusa RH10 and other novel aromatic hybrids with resistance to blast and ShB diseases. The present study demonstrates successful use of a restricted backcrossing strategy for introgression of multiple loci conferring resistance to two important fungal diseases in rice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Ahn SW, de la Peña RC, Candole BL, Mew TW (1986) A new scale for rice sheath blight disease assessment. Int Rice Res Newsl 11:17

    Google Scholar 

  • Alam MF, Datta K, Abrigo E, Oliva N, Tu J, Virmani SS, Datta SK (1999) Transgenic insect maintainer line (IR68899B) for improvement of hybrid rice. Plant Cell Rep 18:572–575

    Article  CAS  Google Scholar 

  • Basavaraj SH, Singh VK, Singh A, Singh D, Nagarajan M, Mohapatra T, Prabhu KV, Singh AK (2009) Marker aided improvement of Pusa 6B, the maintainer parent of hybrid Pusa RH10, for resistance to bacterial blight. Indian J Genet 69:10–16

    CAS  Google Scholar 

  • Basavaraj SH, Singh VK, Singh A, Singh A, Singh A, Yadav S, Ellur RK, Singh D, Gopalakrishnan S, Nagarajan M, Mohapatra T, Prabhu KV, Singh AK (2010) Marker-assisted improvement of bacterial blight resistance in parental lines of Pusa RH10, a superfine grain aromatic rice hybrid. Mol Breed 26:293–305

    Article  CAS  Google Scholar 

  • Bonman JM, Dedios TIV, Khin MM (1986) Physiologic specialization of Pyricularia oryzae in the Philippines. Plant Dis 70:767–769

    Article  Google Scholar 

  • Chahal SS, Sokhi SS, Ratan GS (2003) Investigation on sheath blight of rice in Punjab. Indian Phytopathol 56:22–26

    Google Scholar 

  • Channamallikarjuna V, Sonah H, Prasad M, Rao GJN, Chand S, Upreti HC, Singh NK, Sharma TR (2010) Identification of major quantitative trait loci qSBR11-1 for sheath blight resistance in rice. Mol Breed 25:155–166

    Article  CAS  Google Scholar 

  • Chen J, Li X, Cheng C, Wang Y, Qin M, Zhu H, Zeng R, Fu X, Liu Z, Zhang G (2014) Characterization of epistatic interaction of QTLs LH8 and EH3 controlling heading date in rice. Sci Rep 4:4263. doi:10.1038/srep04263

    PubMed Central  PubMed  Google Scholar 

  • Collard BCY, Mackill DJ (2008) Marker-assisted selection: an approach for precision plant breeding in the twenty-first century. Phil Trans R Soc B 363:557–572

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Datta K, Baisakh N, Thet MK, Tu J, Datta SK (2002) Pyramiding transgenes for multiple resistance in rice against bacterial blight, yellow stem borer and sheath blight. Theor Appl Genet 106:1–8

    CAS  PubMed  Google Scholar 

  • Gangopadhyay S, Chakrabarti NK (1982) Sheath blight of rice. Rev Plant Pathol 61:451–460

    Google Scholar 

  • Gopalakrishnan S, Sharma RK, Rajkumar KA, Joseph M, Singh VP, Singh AK, Bhat KV, Singh NK, Mohapatra T (2008) Integrating marker assisted background analysis with foreground selection for identification of superior bacterial blight resistant recombinants in Basmati rice. Plant Breed 127:131–139

    Article  CAS  Google Scholar 

  • Heuer S, Chin JH, Gamuyao R, Haefele SM, Wissuwa M (2013) Molecular breeding for phosphorus-efficient rice. In: Varshney RK, Tuberosa R (eds) Translational genomics for crop breeding, volume II: abiotic stress, yield and quality. Wiley, New York, pp 65–82

    Chapter  Google Scholar 

  • IRRI (2002) Standard evaluation system for rice (SES). International Rice Research Institute, Los Banos, Philippines, p 56

    Google Scholar 

  • Islam MR, Gregorio GB (2013) Progress of salinity tolerant rice variety development in Bangladesh. SABRAO J 45:21–30

    Google Scholar 

  • Jia Y, Liu G, Costanzo S, Lee S, Dai Y (2009) Current progress on genetic interactions of rice with rice blast and sheath blight fungi. Front Agric China 3:231–239

    Article  CAS  Google Scholar 

  • Joseph M, Gopalakrishnan S, Sharma RK, Singh AK, Singh VP, Singh NK, Mohapatra T (2004) Combining bacterial blight resistance and Basmati quality characteristics by phenotypic and molecular marker assisted selection in rice. Mol Breed 13:377–387

    Article  CAS  Google Scholar 

  • Kato H, Imbe T, Tsunematsu H, Fukuta Y (2004) Develo** rice blast differential lines and evaluating partial resistance for the breeding of durable rice varieties in the tropics. In: Nozoe T, Fukuta Y, Hardy B (eds) Blessings from nature and science for the future. Proceedings No. 10. International Rice Research Institute, Los Banos, Philippines, pp 31–44

  • Kumar A, Dixit S, Henry A (2013) Marker-assisted introgression of major QTLs for grain yield under drought in rice. In: Varshney RK, Tuberosa R (eds) Translational genomics for crop breeding, volume II: abiotic stress, yield and quality. Wiley, New York, pp 47–64

    Chapter  Google Scholar 

  • Malmberg RL, Mauricio R (2005) QTL-based evidence for the role of epistasis in evolution. Genet Res (Camb) 86:89–95

    Article  CAS  Google Scholar 

  • Moreau L, Charcosset A, Hospital F, Gallais A (1998) Marker-assisted selection efficiency in populations of finite size. Genetics 148:1353–1365

    PubMed Central  CAS  PubMed  Google Scholar 

  • Neeraja C, Maghirang-Rodriguez R, Pamplona A, Heuer S, Collard B, Septiningsih E, Vergara G, Sanchez D, Xu K, Ismail AM, Mackill DJ (2007) A marker-assisted backcross approach for develo** submergence-tolerance rice cultivars. Theor Appl Genet 115:767–776

    Article  CAS  PubMed  Google Scholar 

  • Ou SH (1985) Rice diseases, 2nd edn. Commonwealth Mycological Institute, Kew, Surrey, England, p 379p

    Google Scholar 

  • Pan XB, Chen ZX, Xu JY, Tong YH, Wang ZB, Pan XY (1997) The effect of different methods of inoculation and investigation on genetic research of resistance to rice sheath blight. J Jiangsu Agri College 18:27–32

    Google Scholar 

  • Pandey MK, Rani NS, Sundaram RM, Laha GS, Madhav MS, Rao KS, Sudharshan I, Hari Y, Varaprasad GS, Rao LVS, Suneetha K, Sivaranjani AKP, Viraktamath BC (2013) Improvement of two traditional Basmati rice varieties for bacterial blight resistance and plant stature through morphological and marker-assisted selection. Mol Breed 31:239–246

  • Pandian RTP, Sharma P, Singh VK, Singh A, Ellur RK, Singh AK, Singh UD (2012) Validation of sheath blight resistance derived from Tetep in a Basmati variety and parental lines of rice hybrid. Indian Phytopathol 65:233–237

    Google Scholar 

  • Pinson SRM, Capdevielle FM, Oard JH (2005) Confirming QTLs and finding additional loci conditioning sheath blight resistance in rice (Oryza sativa L.) using recombinant inbred lines. Crop Sci 45:503–510

    Article  CAS  Google Scholar 

  • Prabhu KV, Somers DJ, Rakow G, Gugel RK (1998) Molecular markers linked to white rust resistance in mustard Brassica juncea. Theor Appl Genet 97:865–870

    Article  CAS  Google Scholar 

  • Savary S, Teng PS, Willocquet L, Nutter FWJ (2006) Quantification and modeling of crop losses: a review of purpose. Ann Rev Phytopathol 44:89–112

    Article  CAS  Google Scholar 

  • Sharma TR, Chauhan RS, Singh BM, Paul R, Sagar V, Rathore R (2002) RAPD and pathotype analysis of Magnaporthe grisea population from North-western Himalayan region of India. J Phytopathol 150:649–656

    Article  CAS  Google Scholar 

  • Sharma TR, Madhav MS, Singh BK, Shanker P, Jana TK, Dalal V, Pandit A, Singh A, Gaikwad K, Upreti HC, Singh NK (2005) High resolution map**, cloning and molecular characterization of the Pi-k hgene of rice, which confers resistance to M. grisea. Mol Gen Genom 274:569–578

    Article  CAS  Google Scholar 

  • Sharma TR, Rai AK, Gupta GK, Singh NK (2010) Broad-spectrum blast resistance gene Pi-K h cloned from the rice line Tetep designated as Pi54. J Plant Biochem Biotech 19:87–89

    Article  CAS  Google Scholar 

  • Singh AK, Gopala Krishnan S, Singh VP, Prabhu KV, Mohapatra T, Singh NK, Sharma T, Nagarajan M, Vinod KK, Singh D, Singh UD, Chander S, Atwal SS, Seth R, Singh VK, Ellur RK, Singh A, Anand D, Khanna A, Yadav S, Goel N, Singh A, Shikari AB, Singh A, Marathi B (2011) Marker assisted selection: a paradigm shift in Basmati breeding. Indian J Genet 71:1–9

    Google Scholar 

  • Singh VK, Singh A, Singh SP, Ellur RK, Choudhary V, Sarkhel S, Singh D, Gopala Krishnan S, Nagarajan M, Vinod KK, Singh UD, Rathore R, Prasanthi SK, Agrawal PK, Bhatt JC, Mohapatra T, Prabhu KV, Singh AK (2012a) Incorporation of blast resistance into “PRR78”, an elite Basmati rice restorer line, through marker assisted backcross breeding. Field Crop Res 128:8–16

    Article  Google Scholar 

  • Singh A, Singh VK, Singh SP, Ellur RK, Singh D, Bhowmick K, Gopala Krishnan S, Nagarajan M, Vinod KK, Mohapatra T, Prabhu KV, Singh AK (2012b) Marker aided improvement of Pusa1460 an elite Basmati rice for resistance to Blast diseases. AoB Plants pls029. doi:10.1093/aobpla/pls029

  • Singh VK, Singh A, Singh SP, Ellur RK, Singh D, Gopala Krishnan S, Bhowmick PK, Nagarajan M, Vinod KK, Singh UD, Mohapatra T, Prabhu KV, Singh AK (2013) Marker-assisted simultaneous but stepwise backcross breeding for pyramiding blast resistance genes Piz5 and Pi54 into an elite Basmati rice restorer line ‘PRR78’. Plant Breed 132:486–495

    CAS  Google Scholar 

  • Sreewongchai T, Too**da T, Thanintorn N, Kosawang C, Vanivichit A, Tharreau D, Sirithuniya P (2009) Development of elite indica rice lines with wide spectrum of resistance to Thai blast isolated by pyramiding multiple resistance QTLs. Plant Breed 129:176–180

    Article  Google Scholar 

  • Srinivasachary, Willocquet L, Savary S (2011) Resistance to rice sheath blight (Rhizoctonia solani Kuhn) [(teleomorph: Thanatephorus cucumeris (A.B. Frank) Donk.] disease: current status and perspectives. Euphytica 178:1–22

    Article  Google Scholar 

  • Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP map** in plant breeding: new tools for an old science. Biotechnology 7:257–264

    Article  CAS  Google Scholar 

  • Tinker NA, Mather DE (1995) Methods for QTL analysis with progeny replicated in multiple environments. J Agric Genom 1:1–16

    Google Scholar 

  • Wang Y, Cheng LR, Sun Y, Zhou Z, Zhu LH, Xu ZJ, Xu JL (2009) Effect of genetic background on QTLs for heading date and plant height and interactions between QTL and environment using reciprocal introgression lines in rice. Acta Agron Sin 35:1386–1394

    CAS  Google Scholar 

  • Yuan LP (1998) Hybrid rice development and use: innovative approach and challenges. IRC Newsl 47:7–14

    Google Scholar 

  • Zaman FU, Viraktamath BC, Virmani SS (2003) Improving grain quality in hybrid rice. In: Virmani SS, Mao CX, Hardy B (eds) Hybrid rice for food security, poverty alleviation and environmental protection. Manila, International Rice Research Institute, pp 69–82

    Google Scholar 

  • Zuo S, Zhang L, Wang H, Yin Y, Zhang Y, Chen Z, Ma Y, Pan X (2008) Prospect of the QTL-qSB-9Tq utilized in molecular breeding program of japonica rice against sheath blight. J Genet Genom 35:499–505

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The present study was supported under the Indian Council of Agriculture Research, New Delhi funded Network project on gene pyramiding in rice. The virulent isolates of Magnaporthae oryzae were collected under the ICAR-NAIP funded project entitled “Allele Mining and Expression Profiling of Resistance and Avirulence Genes in Rice-Blast Pathosystem for Development of Race Non-Specific Disease Resistance” (NAIP/C4/C1071).

Author information

Authors and Affiliations

  1. Division of Genetics, Indian Agricultural Research Institute, Pusa, New Delhi, 110012, India

    Ashok K. Singh, Vikas K. Singh, Atul Singh, Ranjith K. Ellur, S. Gopala Krishnan & K. V. Prabhu

  2. Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India

    R. T. P. Pandian & U. D. Singh

  3. Rice Breeding and Genetics Research Centre, Indian Agricultural Research Institute, Aduthurai, 612101, India

    M. Nagarajan & K. K. Vinod

  4. International Crop Research Institute for Semi-Arid Tropics (ICRISAT), Patancheru, 502324, India

    Vikas K. Singh

Authors
  1. Ashok K. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vikas K. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Atul Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ranjith K. Ellur
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. T. P. Pandian
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. Gopala Krishnan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. U. D. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. M. Nagarajan
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. K. K. Vinod
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. K. V. Prabhu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ashok K. Singh.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Singh, A.K., Singh, V.K., Singh, A. et al. Introgression of multiple disease resistance into a maintainer of Basmati rice CMS line by marker assisted backcross breeding. Euphytica 203, 97–107 (2015). https://doi.org/10.1007/s10681-014-1267-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-014-1267-1

Keywords

Search

Navigation