Abstract
The common bean is a legume crop distributed worldwide. Dry bean production has gone through increasing difficulties due to relatively low yields in the last few years. Rhizoctonia solani is one of the root and hypocotyl pathogens that causes most of the economic losses in this crop. One promising strategy to control plant diseases is the use of biological control agents, able to reduce the negative effects of pathogens and to promote positive responses in the plant. Trichoderma spp. is a fungal genus ubiquitous in soil that can grow in soil or in any of the above ground parts of plants. The aims of this work were to study the effect of Trichoderma on bean plant growth, in the presence of the phytopathogen R. solani, according to the Trichoderma isolation source (seed or soil). Fifty-five Trichoderma isolates collected from bean seeds and from bean field soils were analyzed. Among them, those isolated from soil samples showed a higher plant growth promotion activity than those strains isolated from seeds, in the presence of R. solani. Furthermore, bean plants inoculated with Trichoderma-soil isolates showed a higher percentage of germination, hypocotyl diameter, length of the root system, and dry weight of aerial parts and root system than plants inoculated with Trichoderma-seed isolates.
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03 December 2020
A Correction to this paper has been published: https://doi.org/10.1007/s10658-020-02174-8
References
Anees, M., Tronsmo, A., Edel-Hermann, V., Hjeljord, L. G., Héraud, C., & Steinberg, C. (2010). Characterization of field isolates of Trichoderma antagonistic against Rhizoctonia solani. Fungal Biology, 114(9), 691–701. https://doi.org/10.1016/j.funbio.2010.05.007.
Benítez, T., Rincón, A. M., Limón, M. C., & Codón, A. C. (2004). Biocontrol mechanisms of Trichoderma strains. International Microbiology, 7(4), 249–260. https://doi.org/10.2436/IM.V7I4.9480.
Brimner, T. A., & Boland, G. J. (2003). A review of the non-target effects of fungi used to biologically control plant diseases. Agriculture, Ecosystems & Environment, 100(1), 3–16. https://doi.org/10.1016/S0167-8809(03)00200-7.
Cardoza, R. E., Hermosa, M. R., Vizcaíno, J. A., González, F., Llobell, A., Monte, E., & Gutiérrez, S. (2007). Partial silencing of a hydroxy-methylglutaryl-CoA reductase-encoding gene in Trichoderma harzianum CECT 2413 results in a lower level of resistance to lovastatin and lower antifungal activity. Fungal Genetics and Biology, 44(4), 269–283. https://doi.org/10.1016/j.fgb.2006.11.013.
Casquero, P. A., Lema, M., Santalla, M., & De Ron, A. M. (2006). Performance of common bean (Phaseolus vulgaris L.) landraces from Spain in the Atlantic and Mediterranean environments. Genetic Resources and Crop Evolution, 53(5), 1021–1032. https://doi.org/10.1007/s10722-004-7794-1.
De Corato, U. (2020). Disease-suppressive compost enhances natural soil suppressiveness against soil-borne plant pathogens: A critical review. Rhizosphere. Elsevier B.V. https://doi.org/10.1016/j.rhisph.2020.100192.
Druzhinina, I. S., Seidl-Seiboth, V., Herrera-Estrella, A., Horwitz, B. A., Kenerley, C. M., Monte, E., Mukherjee, P. K., Zeilinger, S., Grigoriev, I. V., & Kubicek, C. P. (2011). Trichoderma: The genomics of opportunistic success. Nature Reviews Microbiology, 9(10), 749–759.
El-Debaiky, S. A. (2017). Antagonistic studies and hyphal interactions of the new antagonist Aspergillus piperis against some phytopathogenic fungi in vitro in comparison with Trichoderma harzianum. Microbial Pathogenesis, 113, 135–143. https://doi.org/10.1016/J.MICPATH.2017.10.041.
Guerrero-González, M. L., Rodríguez-Kessler, M., Rodríguez-Guerra, R., González-Chavira, M., Simpson, J., Sánchez, F., & Jiménez-Bremont, J. F. (2011). Differential expression of Phaseolus vulgaris genes induced during the interaction with Rhizoctonia solani. Plant Cell Reports, 30(8), 1465–1473. https://doi.org/10.1007/s00299-011-1055-5.
Harman, Gary E, & Kubicek, C. P. (1998). Trichoderma and Gliocladium. Volume 2: Enzymes, Biological Control and commercial applications (Vol. 2). United Kindong: CRC Press.
Harman, G. E., Howell, C. R., Viterbo, A., Chet, I., & Lorito, M. (2004). Trichoderma species - opportunistic, avirulent plant symbionts. Nature reviews. Microbiology, 2(1), 43–56. https://doi.org/10.1038/nrmicro797.
Héraux, F. M. G., Hallett, S. G., Ragothama, K. G., & Weller, S. C. (2005a). Composted chicken manure as a medium for the production and delivery of Trichoderma virens for weed control. HortScience, 40(5), 1394–1397.
Héraux, F. M. G., Hallett, S. G., & Weller, S. C. (2005b). Combining Trichoderma virens-inoculated compost and a rye cover crop for weed control in transplanted vegetables. Biological Control, 34(1), 21–26. https://doi.org/10.1016/J.BIOCONTROL.2005.04.003.
Hermosa, R., Viterbo, A., Chet, I., & Monte, E. (2012). Plant-beneficial effects of Trichoderma and of its genes. Microbiology, 158(1), 17–25. https://doi.org/10.1099/mic.0.052274-0.
Hermosa, R., Cardoza, R. E., Rubio, M. B., Gutiérrez, S., & Monte, E. (2014). Secondary metabolism and antimicrobial metabolites of Trichoderma. In V. K. Gupta, M. Schmoll, A. Herrera-Estrella, R. S. Upadhyay, I. Druzhinina, & M. G. Tuohy (Eds.), Biotechnology and biology of Trichoderma (pp. 125–137). Elsevier B.V. https://doi.org/10.1016/B978-0-444-59576-8.00010-2.
Hutchinson, C. M. (1999). Trichoderma virens-inoculated composted chicken manure for biological weed control. Biological Control, 16(2), 217–222. https://doi.org/10.1006/BCON.1999.0759.
Javaid, A., & Ali, S. (2011a). Alternative management of a problematic weed of wheat Avena fatua L. by metabolites of Trichoderma. Chilean Journal of Agricultural Research, 71(2), 205–211. https://doi.org/10.4067/S0718-58392011000200004.
Javaid, A., & Ali, S. (2011b). Herbicidal activity of culture filtrates of Trichoderma spp. against two problematic weeds of wheat. Natural Product Research, 25(7), 730–740. https://doi.org/10.1080/14786419.2010.528757.
Jiang, Y., Liang, Y., Li, C., Wang, F., Sui, Y., Suvannang, N., Zhou, J., & Sun, B. (2016). Crop rotations alter bacterial and fungal diversity in paddy soils across East Asia. Soil Biology and Biochemistry, 95, 250–261. https://doi.org/10.1016/j.soilbio.2016.01.007.
Johnson, N. C., Wilson, G. W. T., Bowker, M. A., Wilson, J. A., & Miller, R. M. (2010). Resource limitation is a driver of local adaptation in mycorrhizal symbioses. Proceedings of the National Academy of Sciences of the United States of America, 107(5), 2093–2098. https://doi.org/10.1073/pnas.0906710107.
Mayo, S., Gutierrez, S., Malmierca, M. G., Lorenzana, A., Campelo, M. P., Hermosa, R., & Casquero, P. A. (2015). Influence of Rhizoctonia solani and Trichoderma spp. in growth of bean (Phaseolus vulgaris L.) and in the induction of plant defense-related genes. Frontiers in Plant Science, 6, 685. https://doi.org/10.3389/fpls.2015.00685.
Mayo, S., Cominelli, E., Sparvoli, F., González-López, O., Rodríguez-González, A., Gutiérrez, S., & Casquero, P. A. (2016). Development of a qPCR strategy to select bean genes involved in plant defense response and regulated by the Trichoderma velutinum – Rhizoctonia solani interaction. Frontiers in Plant Science, 7, 1109. https://doi.org/10.3389/fpls.2016.01109.
Papavizas, G. C. (1985). Trichoderma and Gliocladium: Biology, ecology, and potential for biocontrol. Annual Review of Phytopathology, 23(1), 23–54.
Restovich, S. B., Andriulo, A. E., & Portela, S. I. (2012). Introduction of cover crops in a maize–soybean rotation of the humid pampas: Effect on nitrogen and water dynamics. Field Crops Research, 128, 62–70. https://doi.org/10.1016/J.FCR.2011.12.012.
Rigaud, J. R., & Puppo, A. (1975). Indole 3 acetic acid catabolism by soybean bacteroids. Journal of General Microbiology, 88(2), 223–228. https://doi.org/10.1099/00221287-88-2-223.
Rivera, A., Casquero, P. A., Mayo, S., Almirall, A., Plans, M., Simó, J., et al. (2016). Culinary and sensory traits diversity in the Spanish core collection of common beans (Phaseolus vulgaris L.). Spanish Journal of Agricultural Research, 14(1), e0701. https://doi.org/10.5424/sjar/2016141-7726.
Sain, S. K., & Pandey, A. K. (2018). Evaluation of some Trichoderma harzianum isolates for the management of soilborne diseases of brinjal and okra. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 88(3), 905–914. https://doi.org/10.1007/s40011-016-0824-x.
Sevim, A., Höfte, M., & Demirbag, Z. (2012). Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. anisopliae isolates obtained from the eastern Black Sea region of Turkey. Turkish Journal of Biology, 36, 255–265. https://doi.org/10.3906/biy-1009-118.
Shoresh, M., Harman, G. E., & Mastouri, F. (2010). Induced systemic resistance and plant responses to fungal biocontrol agents. Annual Review of Phytopathology, 48, 21–43. https://doi.org/10.1146/annurev-phyto-073009-114450.
Tiemann, L. K., Grandy, A. S., Atkinson, E. E., Marin-Spiotta, E., & Mcdaniel, M. D. (2015). Crop rotational diversity enhances belowground communities and functions in an agroecosystem. Ecology Letters, 18(8), 761–771. https://doi.org/10.1111/ele.12453.
Valenciano, J. B., Casquero, P. A., Boto, J. A., & Marcelo, V. (2006). Evaluation of the occurrence of root rots on bean plants (Phaseolus vulgaris) using different sowing methods and with different techniques of pesticide application. New Zealand Journal of Crop and Horticultural Science, 34(4), 291–298. https://doi.org/10.1080/01140671.2006.9514419.
Vinale, F., Sivasithamparam, K., Ghisalberti, E. L., Marra, R., Woo, S. L., & Lorito, M. (2008). Trichoderma–plant–pathogen interactions. Soil Biology and Biochemistry, 40(1), 1–10. https://doi.org/10.1016/j.soilbio.2007.07.002.
Zhang, J., Chen, G.-Y., Li, X.-Z., Hu, M., Wang, B.-Y., Ruan, B.-H., Zhou, H., Zhao, L. X., Zhou, J., Ding, Z. T., & Yang, Y. B. (2017). Phytotoxic, antibacterial, and antioxidant activities of mycotoxins and other metabolites from Trichoderma sp. Natural Product Research, 31(23), 2745–2752. https://doi.org/10.1080/14786419.2017.1295235.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by S. Mayo-Prieto, M.P. Campelo, A. Lorenzana and A. Rodríguez-González. The first draft of the manuscript was written by S. Mayo-Prieto and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Bean-seeds samples collected from PGI “Alubia La Bañeza-León”. A) Select seeds; B) Discarded seeds; C) Sampling in bean warehouse; D) Seed samples in Petri dish on PDA medium; E) Trichoderma spp. in bean seed after 15 days of incubation in PDA medium (JPG 76 kb)
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Mayo-Prieto, S., Campelo, M.P., Lorenzana, A. et al. Antifungal activity and bean growth promotion of Trichoderma strains isolated from seed vs soil. Eur J Plant Pathol 158, 817–828 (2020). https://doi.org/10.1007/s10658-020-02069-8
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DOI: https://doi.org/10.1007/s10658-020-02069-8