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Human gut commensal bacterium Ruminococcus species FMB-CY1 completely degrades the granules of resistant starch

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Abstract

Resistant starch (RS) in the diet reaches the large intestine and is fermented by the gut microbiota, providing beneficial effects on human health. The human gut bacterium FMB-CY1 was isolated and identified as a new species closest to Ruminococcus bromii. Ruminococcus sp. FMB-CY1 completely degraded RS including commercial RS types 2, 3, and 4, and generated glucose and maltose; however, it did not assimilate glucose. Genome analysis revealed 15 amylolytic enzymes (Amy) present in FMB-CY1. The evolutionary trees revealed that the Amys were well divided each other. All Amys (4, 9, 10, 12, and 16) containing cohesin and/or dockerin and scaffolding proteins known to be involved in constituting the amylosome, were identified. A new species of Ruminococcus, strain FMB-CY1, was considered to have the ability to form amylosomes for the degradation of RS. This new RS-degrading Ruminococcus species provides insights into the mechanism(s) underlying RS degradation in the human gut.

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Acknowledgements

This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (2021R1A4A1023437).

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Author notes

  1. Yeong-Sik Hong and Dong-Hyun Jung contributed equally to this work.

Authors and Affiliations

  1. Department of Food Science and Biotechnology, Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin, 17104, Republic of Korea

    Yeong-Sik Hong, Ye-** Kim & Cheon-Seok Park

  2. Microorganism Resources Division, National Institute of Biological Resources, Incheon, 22689, Republic of Korea

    Dong-Hyun Jung

  3. Research Group of Healthcare, Korea Food Research Institute, Wanju, 55365, Republic of Korea

    Won-Hyong Chung & Young-Do Nam

  4. Department of Food Biotechnology, Korea University of Science and Technology, Daejeon, 34113, Republic of Korea

    Young-Do Nam

  5. Department of Food Science and Technology, College of Agriculture and Life Sciences, Jeonbuk National University, Jeonju, 54896, Republic of Korea

    Dong-Ho Seo

  6. Department of Agricultural Convergence Technology, Jeonbuk National University, Jeonju, 54896, Republic of Korea

    Dong-Ho Seo

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  1. Yeong-Sik Hong
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Correspondence to Cheon-Seok Park.

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All applicable international, national, and/or institutional guidelines for the care and use of human derivatives were followed. The study of human derivatives was approved by the Institutional Review Board of Kyung Hee University (KHSIRB-17–004).

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Hong, YS., Jung, DH., Chung, WH. et al. Human gut commensal bacterium Ruminococcus species FMB-CY1 completely degrades the granules of resistant starch. Food Sci Biotechnol 31, 231–241 (2022). https://doi.org/10.1007/s10068-021-01027-2

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  • DOI: https://doi.org/10.1007/s10068-021-01027-2

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