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Complete genome sequence of Bifidobacterium adolescentis P2P3, a human gut bacterium possessing strong resistant starch-degrading activity

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Abstract

Resistant starch (RS) is an important food source from which gut bacteria produce short chain fatty acids, which have beneficial effects for human health. The Bifidobacterium adolescentis P2P3, a human gut bacterium possessing a strong RS-degrading activity, was isolated from a healthy Korean adult male. In vitro experiments showed that this bacterium could utilize approximately 63% of high amylose corn starch after forming RS granule clusters. Here we provide the first complete set of genomic information on RS-degrading B. adolescentis P2P3. The genome of B. adolescentis P2P3 consists of one chromosome (2,202,982 bp) with high GC content (59.4%). Analysis of the protein-coding genes revealed that at least nineteen of the starch degradation-related enzymes were present in the genome. Among those, five genes evidently possess carbohydrate-binding domains, which are presumed to be involved in efficient RS decomposition. The complete set of genomic information on B. adolescentis P2P3 could provide an understanding of the role of RS-degrading gut bacteria and its RS degradation mechanism.

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Acknowledgements

This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (No. 2017R1A2B4004218).

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Authors and Affiliations

  1. Bacteria Research Team, Nakdonggang National Institute of Biological Resources, Sangju, 37242, Republic of Korea

    Dong-Hyun Jung

  2. Research Group of Healthcare, Korea Food Research Institute, Wanju, 55365, Republic of Korea

    Won-Hyong Chung & Young-Do Nam

  3. Department of Food Science and Technology, College of Agriculture and Life Sciences, Jeonbuk National University, Jeonju, 54896, Republic of Korea

    Dong-Ho Seo

  4. Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin, 17104, Republic of Korea

    Ye-** Kim & Cheon-Seok Park

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  1. Dong-Hyun Jung
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  2. Won-Hyong Chung
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  4. Ye-** Kim
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  5. Young-Do Nam
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Contributions

C-S P designed and coordinated all the experiments; D-H J performed the experiments, analyzed the genome data and wrote the manuscript; W-H C, Y-D N and D-H S performed the genome sequencing and sequence assembly; Y-J K measured residual RS. All authors have read and approved the final manuscript.

Corresponding author

Correspondence to Cheon-Seok Park.

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Conflict of interest

The authors have no conflicts of interest to declare.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of human derivatives were followed. The study of human derivatives was approved by the Institutional Review Board of Kyung Hee University (KHSIRB-17-004).

Additional information

Nucleotide sequence accession number: the complete chromosomal sequence of B. adolescentis P2P3 was deposited into Genbank under the Accession Number CP024959.

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Jung, DH., Chung, WH., Seo, DH. et al. Complete genome sequence of Bifidobacterium adolescentis P2P3, a human gut bacterium possessing strong resistant starch-degrading activity. 3 Biotech 10, 31 (2020). https://doi.org/10.1007/s13205-019-2019-7

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  • DOI: https://doi.org/10.1007/s13205-019-2019-7

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