Abstract
Neuromelanin is a black-brownish pigment, present in so-called neuromelanin granules (NMGs) in the cell bodies of dopaminergic neurons in the substantia nigra (SN) pars compacta. These neurons are lost in neurodegenerative diseases, such as Parkinson’s disease and dementia with Lewy bodies. Although it is known that lipids, proteins, and environmental toxins accumulate in NMGs, the function of NMGs has not yet been finally clarified as well as their origin and the synthesis of neuromelanin. We, therefore, isolated NMGs and surrounding SN tissue from control patients by laser microdissection and analyzed the proteomic profile by tandem mass spectrometry. With our improved workflow, we were able to (1) strengthen the regularly reported link between NMGs and lysosomes, (2) detect tyrosine hydroxylase to be highly abundant in NMGs, which may be related to neuromelanin synthesis and (3) indicate a yet undescribed link between stress granules (SGs) and NMGs. Based on our findings, we cautiously hypothesize, that SGs may be the origin of NMGs or form in close proximity to them, potentially due to the oxidative stress caused by neuromelanin-bound metals.
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Introduction
Neuromelanin (NM) is a black-brownish pigment, mainly observable in the cell bodies of dopaminergic neurons in the substantia nigra (SN) pars compacta. In the human brain, NM is stored in membrane-surrounded neuromelanin granules (NMGs) in association with proteins and lipids. These NMGs are first observable in infants while increasing in number and density of pigmentation during aging (Halliday et al. 2006). Investigating the molecular composition of NMGs is of high relevance, since the selective loss of NMG-containing dopaminergic neurons in the SN leads to cardinal symptoms in neurodegenerative diseases, such as Parkinson’s disease (PD) and dementia with Lewy bodies (DLB). Therefore, robust and reproducible OMICs techniques are of immense value, enabling the study of genes, transcripts, proteins and lipids. Nevertheless, as NMGs are absent in common laboratory animals (Marsden 1961), researchers rely on human post mortem brain tissue for their studies, which are limited as only a static view of NMG composition can be observed. Thus, the mechanism of NM synthesis as well as the origin of NMGs have not been finally clarified (Zecca et al. 2001; Vila 2019). Concerning the NM synthesis, several hypotheses have been raised:
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1.
Due to similarities between NM and melanin present in human skin, one hypothesis believes the enzyme tyrosinase, responsible for melanin synthesis (Raper 1928; Mason 1965; Sánchez-Ferrer et al. 1995), to play an essential role in NM synthesis (Carballo-Carbajal et al. 2019). However, so far, only tyrosinase–mRNA could be detected and no evidence was found for the presence of tyrosinase protein in human SN tissue (Miranda et al. 1984; Xu et al. 1997; Ikemoto et al. 1998; Tief et al. 1998; Tribl et al. 2007).
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2.
A second enzyme believed to potentially play a role in NM synthesis is tyrosine hydroxylase (TH), the rate-limiting enzyme in the catecholamine–synthesis pathway, as NM is mainly restricted to brain regions containing catecholaminergic neurons (Saper and Petito 1982; Gaspar et al. 1983; Halliday et al. 1988; Hirsch et al. 1988). Indeed, the presence of TH protein in human brain tissue was reported in several publications (Mogi et al. 1988; Nagatsu et al. 2019), as TH is commonly used as a marker for dopaminergic neurons in immunological stainings (Robert and Meghan 2012). Nevertheless, TH is also present in the SN of rodents, such as mice and rats, in which no production of NM can be observed (Marsden 1961; Aumann et al. 2011). Even TH-overexpression did not lead to the induction of NM synthesis in mice (Mor et al. 2017).
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3.
A third hypothesis assumes an enzyme-free mechanism in which autooxidation of dopamine is expected to be the main process in NM synthesis (Fornstedt et al. 1986; Sulzer and Zecca 2000; Zucca et al. 2017). However, based on this assumption, a formation of NM should be observable in the SN of rats and mice as well.
Furthermore, first studies elucidating the molecular composition of NMGs on the level of proteins resulted in hypotheses on their potential origin. In-depth proteomic characterization, utilizing either density gradient purification via ultracentrifugation (Tribl et al. 2006; Zucca et al. 2018) or selective enrichment via laser microdissection (Plum et al. 2016), revealed an increased abundance of lysosomal proteins. These findings led to the assumption that NMGs may play a protective role in the human brain shielding toxic compounds, such as metals and environmental toxins from the cytoplasm (D'Amato et al. 1986; Lindquist et al. 1988; Aime et al. 1994; Zecca et al. 1996; Bridelli et al. 1999; Double et al. 2003; Tribl et al. 2006; Bohic et al. 2008; Biesemeier et al. 2016; Plum et al. 2016; Zucca et al. 2018). Other studies state that this neuroprotective role may transition ultimately resulting in neurotoxicity, as the NMG composition of highly toxic products can lead to neuroinflammation when released (Oberländer et al. 2011; Zhang et al. 2011; Cebrián et al. 2014). Therefore, the origin and role of NMGs are still widely discussed in the scientific community and need further clarification, as well as the synthesis of NM.
To gain insights into these important aspects of NMG biology, an in-depth characterization of NMGs and SN tissue surrounding NMGs (hereafter called SNSurr.) is indispensable. Therefore, we established a revised and optimized version (Wulf et al. 2021) of our previously published workflow (Plum et al. 2016), optimizing NMG collection and in-depth proteomic characterization, leading to the identification of 170% more proteins and an improved quantification of protein abundances. In total, we were able to identify 2693 proteins in all of our measured samples of SNSurr. tissue and NMGs.
Thus, the present study provides the largest proteomic data set focusing on NMG biology. Concerning NM synthesis, we provide evidence for TH to be of high abundance in NMGs, while we were not able to detect tyrosinase in any of our analyzed samples. In addition, we strengthen the assumed link between NMGs and lysosomes based on their proteomic profile. For the first time, we revealed an enrichment of ribosomal and translation-associated proteins in NMGs, pointing towards a potential new aspect of their biology.
With our results, we contribute to the knowledge of NMG origin and function, opening the possibility to reconsider and extend current hypotheses.
Materials and methods
Ethical statement
Flash frozen, human post mortem SN tissue slices of 5 µm thickness were provided on 1.0 PEN-membrane glass slides (Carl Zeiss Microscopy GmbH, Göttingen, Germany) by the Navarrabiomed Biobank (Pamplona, Spain). The use of human brain tissue was approved by the ethics committee of the Ruhr-University Bochum, Germany (file number 4760-13), according to German regulations and guidelines.
Subjects
SN tissue slices of five control (CTRL) patients, which are identified as controls based on a combination of clinical and pathological measures by the Navarrabiomed Brain Bank, were used. Additional information can be found in Table 1. SN tissue of two patients of the study cohort as well as SN tissue of two additional independent CTRL patients was used for verification experiments (Table 1, highlighted in italic).
Laser microdissection and sample preparation
The SN tissue slices were stored at − 80 °C until further use. The isolation of NMGs and SNSurr. tissue was performed as previously reported (Wulf et al. 2021). In brief, NMGs covering a tissue area of 500,000 µm2 were isolated at 400 x-magnification and collected in water-filled caps of non-adhesive microtubes (MicroTube 500, Carl Zeiss Microscopy GmbH) using a PALM Micro Beam (P.A.L.M.-System, Carl Zeiss Microscopy GmbH). Subsequently, SNSurr. tissue was selected at 50 x-magnification and an area of 1,000,000 µm2 was collected in a fresh tube. All samples were stored at -80 °C until further use.
Sample preparation
The sample preparation was performed as previously reported (Wulf et al. 2021). Briefly, samples were treated with formic acid (FA) and sonicated. After a drying step in a vacuum-centrifuge (Concentrator plus, Eppendorf AG, Hamburg, Germany), samples were refilled with ammonium bicarbonate, reduced with dithiothreitol and acetylated with iodoacetamide. Trypsin was added to the samples according to the collected tissue area and digestion was performed at 37 °C overnight. The digestion was stopped by adding trifluoroacetic acid (TFA). At last, samples were vacuum-dried and peptides were stored in 20 µl of 0.1% TFA at -80 °C.
Mass spectrometric analysis
For liquid-chromatography tandem mass spectrometry (LC–MS/MS) analysis, peptide samples were diluted in 0.1% TFA. To ensure an identical peptide load, the volume of peptide solution was adapted according to the amount of collected tissue. As for each NMG sample 500,000 µm2, and for each SNSurr. sample 1,000,000 µm2 of tissue were collected, 5 µl of peptide solution were used for mass spectrometric measurements of NMG samples, while 2.5 µl were used for the measurements of the SNSurr. samples. Mixtures of all samples were measured over the course of all LC–MS/MS measurements to check for technical variances and total ion chromatogram as well as basepeak intensities were checked to ensure identical sample load. The LC–MS/MS measurements were performed on an Ultimate 3000 RSLC nano LC system (Dionex, Idstein, Germany) coupled to an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific). At first, peptides were loaded on a pre-column (Acclaim PepMap nanoViper, Thermo Fisher Scientific; 100 µm × 2 cm, 5 µm particle size) and washed for 7 min with 0.1% TFA at a flow rate of 30 µl/min. Subsequently, the pre-column was connected to an analytical C18 column (Acclaim PepMap nanoViper, Thermo Fisher Scientific; 75 µm × 50 cm, 2 µm particle size). Separation of peptides was performed at a flow rate of 400 nL/min with a gradient starting with 95% solution A (0.1% FA) and 5% solution B (84% acetonitrile, 0.1% FA). The concentration of solution B was increased up to 30% after 105 min, then within 2 min to 95% and maintained at that level for 3 further minutes. Subsequently, the column was adjusted back to 5% solution B. These gradient settings were used for data dependent acquisition (DDA)-experiments, as well as for parallel reaction monitoring (PRM)-experiments. The nano LC-system was directly coupled to the electrospray ionization source (Thermo Fisher Scientific) of the Orbitrap Fusion Lumos Tribrid mass spectrometer.
Global mass spectrometry
For global DDA-experiments, the system operated within a scan range from 350 to 1400 m/z with a resolution of 120,000 and a maximum injection time of 80 ms. In a fixed cycle time of 2 s, all precursor ions with an intensity above 1 × 104 were selected for fragmentation at a fixed collision energy of 28% by higher energy collisional dissociation (HCD). Precursor ions selected for fragmentation were maintained on a dynamic exclusion list for 30 s. Fragment ion scans were performed at a resolution of 30,000 with a maximum injection time of 80 ms.
Targeted mass spectrometry
For PRM-experiments, the system operated within a scan range of 350 to 1400 m/z with a resolution of 120,000 and a maximum injection time of 50 ms. An inclusion list containing 7 precursor ions, representing unique peptides of tyrosine hydroxylase, glycerol-3-phosphate dehydrogenase and cytoplasmic dynein 1 heavy chain 1 was applied (Table 2). For each peptide precursor, a retention time (RT) window of 3 min was defined in the inclusion list. During the RT window, the peptide precursor was selected by the Quadrupole for fragmentation at a collision energy of 28% by HCD. The fragment ion scan was carried out in the Orbitrap at a resolution of 60,000 with a maximum injection time of 200 ms.
The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE (Perez-Riverol et al. 2022) partner repository with the data set identifier PXD033533.
Data analysis and statistics
Global mass spectrometry
The MaxQuant software (version 1.6.17.0) was used for the analysis of raw files. The resulting peak lists were searched against the human UniProt FASTA reference proteome (version 20th January 2021; 75,796 entries) and a common contaminants database provided in the Andromeda search engine (Cox and Mann 2008; Cox et al. 2011; Tyanova et al. 2016a). Due to sample preparation, carbamidomethlyation of methionine was set as fixed modification and carbamidomethylation of any N-terminus, oxidation of methionine as well as deamidation of asparagine and glutamine were set as variable modifications. Trypsin was set as digestion enzyme and a maximum number of 2 missed cleavages were tolerated in the search. The false discovery rate (FDR) was set to 1% for peptides (minimum length of seven amino acids) and proteins and was determined by searching against a reverse decoy database. Peptide identification was carried out with an initial allowed precursor mass deviation up to 5 parts per million (ppm) and an allowed fragment mass deviation of 20 ppm. Match between runs was enabled to enhance and optimize peptide identification. Label free quantification (LFQ) was performed using the MaxQuant LFQ normalization, whereby the Top10 unique and razor peptides were assessed for protein quantification (Cox et al. 2014). In addition, intensity based absolute quantification (iBAQ) value calculation was enabled. IBAQ values were normalized for each sample and multiplied by 1000 to obtain per mille values. Of these normalized iBAQ values, mean values were created for each sample group (NMG, SNSurr.). Protein groups (hereafter referred to as proteins) with normalized mean iBAQ values > 0 were considered as identified.
The resulting protein group output was analyzed using the Perseus software (version 1.6.15.0), as part of the MaxQuant environment (Tyanova et al. 2016b), whereby master mixes were excluded for the subsequent analysis. Contaminants and decoys were filtered out and LFQ values were subsequently log2 transformed for further statistical analysis. A minimum of 70% valid values per group was set as an additional filtering criterion prior to relative quantification of proteins. Prior to statistical analysis, the remaining missing values were imputed from a normal distribution using a width of 0.3 and a downshift of 1.8. Paired Student’s t test as well as p value correction by Benjamini–Hochberg were applied to the data set (leading to q values) to determine proteins with significantly differential abundances. Proteins with a p value < 0.05 were defined as being significantly differential, while q values were applied as more stringent statistical criteria. Fold changes were determined by calculating the ratio of means between the two assessed groups (NMG, SNSurr.).
The results of this analysis are presented as a Volcano plot, created in R version 4.0.3 (R Foundation for Statistical Computing 2020) using the packages ggplot2 3.3.3 and ggrepel 0.9.1. Negative log10-transformed p values (uncorrected) are plotted against the log2-transformed fold changes. Proteins that reach a corrected p value < 0.05 are marked in red. On each side, the ten proteins with the largest Euclidean distance from the origin in the Volcano plot are labeled with the corresponding gene name.
For principal component analysis (PCA), analysis was conducted and graphics were created in R using the same software versions as for the Volcano plot. LFQ values from MaxQuant were taken and log2-transformed, zero values were treated as missing values. Proteins with more than 50% missing values were excluded and the remaining missing values were imputed by the mean of the corresponding protein intensities. A PCA was calculated using the setting that scales the data beforehand. The first two principal components (PC) were plotted.
Differentially expressed proteins as well as identified proteins in SNSurr. and NMGs based on iBAQ were further analyzed by GO (gene ontology)-term and pathway enrichment analysis. In brief, proteins were used for annotation enrichment using the web version of DAVID Bioinformatics Resources 6.8 (Da Huang et al. 2009a, 2009b) to gain a first overview of differences in the proteomic profile between NMGs and SNSurr. tissue hinting towards characteristic functions. The homo sapiens proteome served as a reference and GO-terms from the categories Biological Process (BP), Cellular Compartment (CC), Molecular Function (MF) and KEGG Pathways were taken for in-depth analysis. A p value < 0.05 was used as a significance threshold and fold enrichment scores were taken to assess the enrichment of resulting GO-terms and pathways. To search for information on specific proteins, the UniProt database was utilized (The UniProt Consortium 2021).
Targeted mass spectrometry
Verification of differentially expressed proteins with significant p values was carried out by PRM experiments for tyrosine hydroxylase, glycerol-3-phosphate dehydrogenase and cytoplasmic dynein 1 heavy chain 1. For that, the resulting peptide output of MaxQuant was processed in an identical manner as our protein group output and peptides assigned to TH were examined for significantly differential expression (p value < 0.05), fold change, retention time and charge state. As housekee** proteins, glycerol-3-phosphate dehydrogenase and cytoplasmic dynein 1 heavy chain 1 were chosen, as they were found to be neither significant nor differential between NMGs and SNSurr. tissue and additionally displayed a low standard deviation on the level of intensities. Evaluation of PRM data was performed using Skyline software (MacLean et al. 2010). Group comparisons were performed on peak areas for each protein and an adjusted p value < 0.05, according to Benjamini–Hochberg, was used as a significance threshold on MS2-level.
Results
Global proteomic characterization of NMGs and surrounding tissue
We first set out to characterize the individual protein profiles of NMGs and SNSurr. tissue (Fig. 1, Supplementary Table 1). As the main components of brain tissue are neurons (in case of SN tissue dopaminergic neurons), oligodendrocytes and astrocytes, we confirmed the high abundance of selected marker proteins for each cell type in NMGs and SNSurr. tissue (Fig. 1, neurons: alpha-synuclein (SNCA), neurofilament light (NEFL), medium (NEFM) and heavy chain (NEFH), amyloid beta precursor protein (APP), microtubule associated protein tau (MAPT); dopaminergic neurons: tyrosine hydroxylase (TH), aromatic-L-amino-acid decarboxylase (DDC), solute carrier family 6 member 3 (SLC6A3); astrocytes: glial fibrillary acidic protein (GFAP), S100 calcium binding protein B (S100B); oligodendrocytes: myelin basic protein (MBP), proteolipid protein 1 (PLP1)).
Especially neuronal and astrocytic markers such as SNCA, NEFL, S100B, NEFH and GFAP were found to be highly abundant in SNSurr. tissue, indicating the presence of astrocytes and neurons in the tissue surrounding NMGs, whereas the dopaminergic marker proteins DDC and TH were of lower abundance.
DDC and TH were instead found to be of higher abundance in NMGs, while both astrocytic markers, GFAP and S100B, were found to be lower abundant in NMGs. Neuronal markers SNCA, NEHL and NEFH showed a similar abundance pattern as in SNSurr. tissue. Marker proteins for oligodendrocytes showed low abundance levels in both sample types, PLP1 was not even identified in NMGs. These findings support the localization of NMGs in cell bodies of dopaminergic neurons and allow the characterization of the SNSurr. tissue as mainly composed of neurons and astrocytes, while oligodendrocytes play only a minor role.
NMGs and surrounding tissue can be separated based on their proteomic profile
As the majority of proteins present in the human brain are not restricted to a single cell type or cell compartment, a proteomic study based only on the identification of proteins may miss important information about NMGs. Therefore, we also included the abundances of proteins in our analysis to gain information about proteins being enriched in either NMGs or SNSurr..
Our data set included 1088 proteins, which suited the criteria for our quantitative comparison (Supplementary Table 2). As the principal component analysis (PCA) in Fig. 2 shows, NMGs and SNSurr. can be distinguished already with a high percentage on Principal Component 1, exceptionally stressing their high individuality on the proteome level.
Differential proteins hint towards an involvement of NMGs in protein synthesis
Since especially proteins with high and significant fold changes may provide insights into characteristics of NMGs and SNSurr. tissue, we focused especially on these proteins when evaluating the results of our quantitative comparison. Indeed, 345 proteins showed a statistically significant differential abundance (adjusted p value < 0.05), displayed in the Volcano Plot (Fig. 3). For further in-depth characterization, we focused on the Top 10 proteins (Fig. 3B, C) regarding high and significant fold changes.
Among these proteins, lysosomal sialate O-acetylesterase (SIAE) showed the highest enrichment in NMGs (Fig. 3C), strengthening the relationship between lysosomes and NMGs. Furthermore, a link to protein synthesis was found, since three tRNA ligases (isoleucine–tRNA ligase (IARS), phenylalanine–tRNA ligase beta subunit (FARSB), arginine–tRNA ligase (RARS)) and three ribosomal proteins (40S ribosomal protein S4 X isoform (RPS4X), 60S ribosomal protein L7a (RPL7A), 40S ribosomal protein S9 (RPS9)) showed higher levels in NMGs.
In contrast, the top ten proteins enriched in SNSurr. tissue (Fig. 3B) were found to be characteristic for neuron-surrounding tissue, such as the myelin-associated glycoprotein (MAG) as a part of the neuronal myelin sheath. Proteins exclusively identified in SNSurr. tissue stressed the neuronal character of the tissue, as for example myelin proteolipid protein, dynein light chain 1 and peripherin were included in this list (Supplementary Table 1).
In summary, our quantitative comparison strengthens the previously reported relation between lysosomes/endosomes and NMGs (Plum et al. 2016; Zucca et al. 2018). Nevertheless, as proteins associated with protein synthesis were found to be significantly higher abundant in NMGs, our analysis may open a new perspective of NMG biology as, to our knowledge, enhanced abundances of ribosomal, mRNA-binding and translation-related proteins in NMGs were never reported before. This finding is supported by the results of a GO-term analysis of proteins exclusively identified in NMGs, which revealed a variety of proteins to be related to ribosomes, mRNA-binding and translation (Supplementary Table 1).
Stress granule-related proteins are significantly higher abundant in NMGs
Especially, the high abundances of proteins of the 40S ribosomal subunit in combination with several eukaryotic translation initiation factors (eIF) and RNA-binding proteins raised the question of a potential link between NMGs and stress granules (SGs). SGs are transient structures, which form after the inhibition or interruption of translation and, therefore, contain the aforementioned protein species (Kedersha et al. 2016).
To further characterize this potential link, we analyzed the results of our quantitative comparison with a special focus on SG-specific proteins based on a previously published list of 37 proteins (Wolozin and Ivanov 2019). Nine well-known SG proteins, amongst them polyadenylate-binding protein 1 (PABPC1), which is frequently used in immunohistochemical stainings as a marker protein for SGs, were significantly higher abundant in NMGs with fold changes between 2.1 and 4.9-fold (Fig. 4).
Due to the strict filter criteria for our quantitative comparison, we also checked our data set for the global proteome analysis. In this, 23 additional SG-specific proteins were included, all of them showing higher abundances in NMGs compared to SNSurr. tissue. In addition, three SG-proteins (Caprin 1, Caprin 2 and probable ATP-dependent RNA helicase DDX5) were exclusively identified in NMGs (Supplementary Table 1).
In summary, our complete data set contains data for 32 of the previously published 37 SG-proteins, of which all are higher abundant in NMGs. Through our quantitative comparison, we provide clear evidence for elemental components of SGs (Kedersha et al. 2005) such as polyadenylate-binding protein 1 (PABPC1), subunits of eukaryotic translation initiation factor 3 (eIF3A, eIF3B) and eIF4 gamma 1 (eIF4G1) being significantly higher abundant in NMGs than in surrounding tissue.
Tyrosine hydroxylase is significantly higher abundant in NMGs
Since the findings presented up to this point were primarily the result of a systematic comparison, we wanted to take another look at those aspects of NMG biology that have already been intensively studied in the past, namely, the involvement in the iron metabolism of the SN and the mechanism of NM synthesis.
Stressing the importance of iron metabolism in the SN, ferritin heavy chain was amongst the highest abundant proteins in both NMGs and SNSurr. tissue, with a significantly higher abundance in SNSurr. tissue (1.8-fold, Supplementary Tables 1, 2), while ferritin light chain was comparably abundant in both of our sample groups as well as serotransferrin.
Concerning NM synthesis, we focused on proteins involved in dopamine metabolism as well as tyrosinase, since it is often assumed to be involved in NM synthesis. Although a highly sensitive state-of-the-art mass spectrometer was used, tyrosinase was not detected in any of our measurements, neither in SNSurr. tissue, nor in NMGs. For proteins involved in dopamine metabolism, we focused on tyrosine hydroxylase (TH) as the rate limiting enzyme in catecholamine biosynthesis and, therefore, essential in the synthesis of dopamine, as well as aromatic-L-amino-acid decarboxylase (DDC), monoamine oxidase A and B (MAOA, MAOB), vesicular monoamine transporter 2 (VMAT2/SLC18A2) and dopamine transporter (DAT/SLC6A3).
In our quantitative comparison, we found both proteins required for dopamine synthesis, TH (fold change: 2.5, q value: 0.03) and DDC (fold change: 3.3; q value: 0.01), to be significantly higher abundant in NMGs. We could further verify the significantly higher abundance of TH in NMGs in independent targeted MS-experiments (Fig. 5), in which only peptides unique for TH were quantified. To ensure identical sample load we used two reference proteins (Glycerol-3-phosphate dehydrogenase, mitochondrial (GPDM) and cytoplasmic dynein 1 heavy chain 1 (DYHC1)), thus verifiying the increased abundance of TH in NMG tissue.
Further proteins associated with dopamine metabolism did not display such a cohesive picture. Conversely, MAOA showed a comparable abundance in SNSurr. tissue and NMGs (fold change: 0.83; q value: 0.18), while MAOB was significantly higher abundant in SNSurr. tissue (fold change: 0.59; q value: 0.04).
VMAT2 was not identified at all in any of our samples. DAT instead did not match the criteria of our quantitative comparison, but protein levels showed a higher abundance in NMGs.
Summarizing our findings, we provide evidence for enzymes required for dopamine synthesis, TH and DDC, being highly abundant in NMGs.
Discussion
Our here presented study represents the largest data set concerning the global proteome of NMGs, including 2693 identified proteins, of which 1088 were examined using a relative quantification approach allowing an in-depth characterization of protein abundances in NMGs and surrounding SN tissue for the first time. Both, identified and quantified proteins, give further insights into open questions of NMG biology concerning origin and function of NMGs as well as NM synthesis.
Origin and function of neuromelanin granules
The origin and function of NMGs are widely discussed. With our proteomic characterization we were able to highlight several key characteristics of the proteomic compound of NMGs, potentially revealing distinct functions in the SN. Until now, the most widely accepted theory regarding NMG origin claims a close relation between NMGs and lysosomes or endosomes (Tribl et al. 2006; Plum et al. 2016; Zucca et al. 2018). This theory is based on the results of several proteomic studies and the observation that NMGs are surrounded by a double membrane, a distinct feature of organelle structures (D'Agostino and Luse 1964; Tribl et al. 2006; Plum et al. 2016; Zucca et al. 2018). In a first approach on characterizing NMGs, we (Plum et al. 2016) further hypothesized that NM may be first generated in the cytosol and then taken up by lysosomes, resulting in NMG formation.
In the present study, we can confirm the link between NMGs and lysosomes based on higher abundances of lysosomal proteins such as SIAE, Lysosome-associated membrane glycoprotein 2 (LAMP2) and several cathepsines in NMGs compared to SNSurr. tissue (Supplementary Tables 1, 2). In addition, the identification of typically non-neuronal proteins such as GFAP in NMGs may be another hint towards their lysosomal role. To ensure, that the presence of these proteins in our samples is not based on contaminations due to our methodological approach, we compared our data set with previously published studies focusing on the proteomic content of NMGs (Plum et al. 2016; Zucca et al. 2018). Since GFAP was identified in NMGs in both studies, although the latter one (Zucca et al. 2018) used an independent methodological approach for the purification of NMGs in density gradient purification via ultracentrifugation, this seems to be a reliable finding.
While these observations would assume that NMGs are a degradative organelle, we are the first to provide evidence for a link between SGs and NMGs, as we detected several well-known SG-associated proteins in the NMGs. From 37 SG proteins (Wolozin and Ivanov 2019), we were able to identify 32 in our data set, all of them being higher abundant in NMGs.
In general, SGs are known to form under external stress and influence mRNA function, localization as well as signaling pathways (Buchan and Parker 2009; Buchan et al. 2013; Kedersha et al. 2013; Wheeler et al. 2016). However, recent studies suggest that the frequently observed aggregates in various neurodegenerative disorders may originate from SGs, which develop into persistent protein aggregates under constant cell stress (Wolozin 2012; Dobra et al. 2018). Indeed, we could find TAR DNA-binding protein 43 (TDP43), which is the main component of protein aggregates in amyotrophic lateral sclerosis (Neumann et al. 2009), to be higher abundant in NMGs than in SNSurr. tissue.
Our data thus indicate that NMGs, similar to protein aggregates in neurodegenerative diseases, contain several SG marker proteins. We, therefore, cautiously raise two possible hypotheses, potentially explaining the relation between SGs and NMGs:
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1. SGs may be the origin of NMGs as a result of chronic cell stress.
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2. SGs form in close proximity to NMGs.
A major stress factor contributing to transient SG formation and potentially their transformation into persistent NMG structures could be oxidative stress caused by NM-bound metals (Sian-Hülsmann et al. 2011; Riederer et al. 2021). It was reported that metal concentrations in SN are high and that metal ions are associated with neuromelanin within NMGs (Zecca and Swartz 1993). In addition, we were able to show that iron-binding proteins such as ferritin light and heavy chain were highly abundant in NMGs, but also in the surrounding tissue, assuming that they are not NMG specific (Supplementary Table 1).
Still, we have to point out that NMGs, unlike SGs, are membrane-surrounded and thus may be closer related to lysosomes, since both inherit misfolded protein species and lyososomal/endosomal proteins (Tribl et al. 2005, 2006; Plum et al. 2016; Zucca et al. 2018). Unfortunately, we are not able to verify our hypotheses with the methods applied in this manuscript. To study the potential link between SGs and NMGs and to commit to one of our hypotheses, functional studies in suitable neuromelanin-like pigments producing models (Hasegawa et al. 2003; Jo et al. 1988).
These findings suggest that production of dopamine may take place in NMGs of the SN, although our data cannot prove the production of dopamine by functional TH and DDC. Thus, this hypothesis needs to be further investigated in the future. Nevertheless, it is known that dopamine not imported into vesicles by VMAT2 either oxidizes into aminochrome, a precursor of neuromelanin, or can be degraded by MAOA (Smythies 1996; Muñoz et al. 2012; Goldstein 2021).
Another interesting finding in this context is the higher abundance of DAT/SLC6A3 in NMGs compared to SNSurr. tissue. Although the mediation of the reuptake of dopamine from the synaptic cleft is the main task of DAT, it is also reported that DAT is able to release dopamine in the extracellular space (Khoshbouei et al. 2003). In addition to its localization in the plasma membrane, DAT was observed to be localized in the soma of dopaminergic neurons at the endoplasmic reticulum, the Golgi apparatus and in “multivesicular body vesicles” (Hersch et al. 1997). Therefore, the localization of DAT in NMGs could indicate a functional role, although the direction of dopamine transport mediated by DAT in NMGs needs further examination.
However, it is important to mention that not all TH-positive neurons in the human SN contain NMGs (Hirsch et al. 1989; Saper and Petito 1982) and that no NM synthesis can be observed in mice or rats, although dopamine is synthesized in their brains.
Conclusions
Concluding, our data provides us with further evidence on NM synthesis and NMG origin. Based on our analysis of the proteomic profile of NMGs and surrounding SN tissue, we hypothesize that SGs resulting from cellular stress may be the origin of NMGs or that they form in close proximity to NMGs. In addition, we provide further evidence for the link of NMGs and lysosomes. Concerning NM synthesis, we hypothesize that NM may be synthesized in a pathway involving TH and autooxidative processes. Still, to verify our findings, functional analyses in appropriate cell culture or animal models are inevitable to further elucidate NMG genesis and function in disease progression.
References
Aime S, Fasano M, Bergamasco B, Lopiano L, Valente G (1994) Evidence for a glycidic-lipidic matrix in human neuromelanin, potentially responsible for the enhanced iron sequestering ability of substantia nigra. J Neurochem 62(1):369–371
Aumann TD, Egan K, Lim J, Boon WC, Bye CR, Chua HK, Baban N, Parish CL, Bobrovskaya L, Dickson P, Horne MK (2011) Neuronal activity regulates expression of tyrosine hydroxylase in adult mouse substantia nigra pars compacta neurons. J Neurochem 116(4):646–658
Biesemeier A, Eibl O, Eswara S, Audinot J-N, Wirtz T, Pezzoli G, Zucca FA, Zecca L, Schraermeyer U (2016) Elemental map** of Neuromelanin organelles of human Substantia Nigra: correlative ultrastructural and chemical analysis by analytical transmission electron microscopy and nano-secondary ion mass spectrometry. J Neurochem 138(2):339–353
Bohic S, Murphy K, Paulus W, Cloetens P, Salomé M, Susini J, Double K (2008) Intracellular chemical imaging of the developmental phases of human neuromelanin using synchrotron X-ray microspectroscopy. Anal Chem 80(24):9557–9566
Bridelli MG, Tampellini D, Zecca L (1999) The structure of neuromelanin and its iron binding site studied by infrared spectroscopy. FEBS Lett 457(1):18–22
Buchan JR, Parker R (2009) Eukaryotic stress granules: the ins and outs of translation. Mol Cell 36(6):932–941
Buchan JR, Kolaitis R-M, Taylor JP, Parker R (2013) Eukaryotic stress granules are cleared by autophagy and Cdc48/VCP function. Cell 153(7):1461–1474
Carballo-Carbajal I, Laguna A, Romero-Giménez J, Cuadros T, Bové J, Martinez-Vicente M, Parent A, Gonzalez-Sepulveda M, Peñuelas N, Torra A, Rodríguez-Galván B, Ballabio A, Hasegawa T, Bortolozzi A, Gelpi E, Vila M (2019) Brain tyrosinase overexpression implicates age-dependent neuromelanin production in Parkinson’s disease pathogenesis. Nat Commun 10(1):973
Cartier EA, Parra LA, Baust TB, Quiroz M, Salazar G, Faundez V, Egaña L, Torres GE (2010) A biochemical and functional protein complex involving dopamine synthesis and transport into synaptic vesicles. J Biol Chem 285(3):1957–1966
Cebrián C, Zucca FA, Mauri P, Steinbeck JA, Studer L, Scherzer CR, Kanter E, Budhu S, Mandelbaum J, Vonsattel JP, Zecca L, Loike JD, Sulzer D (2014) MHC-I expression renders catecholaminergic neurons susceptible to T-cell-mediated degeneration. Nat Commun 5:3633
Cox J, Mann M (2008) MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification. Nat Biotechnol 26(12):1367–1372
Cox J, Michalski A, Mann M (2011) Software lock mass by two-dimensional minimization of peptide mass errors. J Am Soc Mass Spectrom 22(8):1373–1380
Cox J, Hein MY, Luber CA, Paron I, Nagaraj N, Mann M (2014) Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ. MCP 13(9):2513–2526
Da Huang W, Sherman BT, Lempicki RA (2009a) Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists. Nucleic Acids Res 37(1):1–13
Da Huang W, Sherman BT, Lempicki RA (2009b) Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc 4(1):44–57
D’AgostinoLuse AJSA (1964) Electron microscopic observations on the human substantia nigra. Neurology 14:529–536
D’Amato RJ, Lipman ZP, Snyder SH (1986) Selectivity of the parkinsonian neurotoxin MPTP: toxic metabolite MPP+ binds to neuromelanin. Science 231(4741):987–989
Dobra I, Pankivskyi S, Samsonova A, Pastre D, Hamon L (2018) Relation between stress granules and cytoplasmic protein aggregates linked to neurodegenerative diseases. Curr Neurol Neurosci Rep 18(12):107
Double KL, Gerlach M, Schünemann V, Trautwein AX, Zecca L, Gallorini M, Youdim MB, Riederer P, Ben-Shachar D (2003) Iron-binding characteristics of neuromelanin of the human substantia nigra. Biochem Pharmacol 66(3):489–494
Finberg JPM (2019) Inhibitors of MAO-B and COMT: their effects on brain dopamine levels and uses in Parkinson’s disease. J Neural Transm 126(4):433–448
Fornstedt B, Rosengren E, Carlsson A (1986) Occurrence and distribution of 5-S-cysteinyl derivatives of dopamine, dopa and dopac in the brains of eight mammalian species. Neuropharmacology 25(4):451–454
Gaspar P, Berger B, Gay M, Hamon M, Cesselin F, Vigny A, Javoy-Agid F, Agid Y (1983) Tyrosine hydroxylase and methionine-enkephalin in the human mesencephalon. J Neurol Sci 58(2):247–267
Goldstein DS (2021) The catecholaldehyde hypothesis for the pathogenesis of catecholaminergic neurodegeneration: what we know and what we do not know. Int J Mol Sci. https://doi.org/10.3390/ijms22115999
Halliday GM, Li YW, Joh TH, Cotton RG, Howe PR, Geffen LB, Blessing WW (1988) Distribution of monoamine-synthesizing neurons in the human medulla oblongata. J Comp Neurol 273(3):301–317
Halliday GM, Fedorow H, Rickert CH, Gerlach M, Riederer P, Double KL (2006) Evidence for specific phases in the development of human neuromelanin. J Neural Transm 113(6):721–728
Hasegawa T, Matsuzaki M, Takeda A, Kikuchi A, Furukawa K, Shibahara S, Itoyama Y (2003) Increased dopamine and its metabolites in SH-SY5Y neuroblastoma cells that express tyrosinase. J Neurochem 87(2):470–475
Hersch SM, Yi H, Heilman CJ, Edwards RH, Levey AI (1997) Subcellular localization and molecular topology of the dopamine transporter in the striatum and substantia nigra. J Comp Neurol 388(2):211–227
Hirsch E, Graybiel AM, Agid YA (1988) Melanized dopaminergic neurons are differentially susceptible to degeneration in Parkinson’s disease. Nature 334(6180):345–348
Hirsch EC, Graybiel AM, Agid Y (1989) Selective vulnerability of pigmented dopaminergic neurons in Parkinson’s disease. Acta Neurol Scand Suppl 126:19–22
Ikemoto K, Nagatsu I, Ito S, King RA, Nishimura A, Nagatsu T (1998) Does tyrosinase exist in neuromelanin-pigmented neurons in the human substantia nigra? Neurosci Lett 253(3):198–200
Jo J, **ao Y, Sun AX, Cukuroglu E, Tran H-D, Göke J, Tan ZY, Saw TY, Tan C-P, Lokman H, Lee Y, Kim D, Ko HS, Kim S-O, Park JH, Cho N-J, Hyde TM, Kleinman JE, Shin JH, Weinberger DR, Tan EK, Je HS, Ng H-H (2016) Midbrain-like organoids from human pluripotent stem cells contain functional dopaminergic and neuromelanin-producing neurons. Cell Stem Cell 19(2):248–257
Kedersha N, Stoecklin G, Ayodele M, Yacono P, Lykke-Andersen J, Fritzler MJ, Scheuner D, Kaufman RJ, Golan DE, Anderson P (2005) Stress granules and processing bodies are dynamically linked sites of mRNP remodeling. J Cell Biol 169(6):871–884
Kedersha N, Ivanov P, Anderson P (2013) Stress granules and cell signaling: more than just a passing phase? Trends Biochem Sci 38(10):494–506
Kedersha N, Panas MD, Achorn CA, Lyons S, Tisdale S, Hickman T, Thomas M, Lieberman J, McInerney GM, Ivanov P, Anderson P (2016) G3BP-Caprin1-USP10 complexes mediate stress granule condensation and associate with 40S subunits. J Cell Biol 212(7):845–860
Khoshbouei H, Wang H, Lechleiter JD, Javitch JA, Galli A (2003) Amphetamine-induced dopamine efflux. A voltage-sensitive and intracellular Na+-dependent mechanism. J Biol Chem 278(14):12070–12077
Konradi C, Svoma E, Jellinger K, Riederer P, Denney R, Thibault J (1988) Topographic immunocytochemical map** of monoamine oxidase-A, monoamine oxidase-B and tyrosine hydroxylase in human post mortem brain stem. Neuroscience 26(3):791–802
Lindquist NG, Larsson BS, Lydén-Sokolowski A (1988) Autoradiography of 14Cparaquat or 14Cdiquat in frogs and mice: accumulation in neuromelanin. Neurosci Lett 93(1):1–6
MacLean B, Tomazela DM, Shulman N, Chambers M, Finney GL, Frewen B, Kern R, Tabb DL, Liebler DC, MacCoss MJ (2010) Skyline: an open source document editor for creating and analyzing targeted proteomics experiments. Bioinformatics 26(7):966–968
Marsden CD (1961) Pigmentation in the nucleus substantiae nigrae of mammals. J Anat 95:256–261
Mason HS (1965) Oxidases. Annu Rev Biochem 34:595–634
Miranda M, Botti D, Bonfigli A, Ventura T, Arcadi A (1984) Tyrosinase-like activity in normal human substantia nigra. Gen Pharmacol 15(6):541–544
Mogi M, Harada M, Kiuchi K, Kojima K, Kondo T, Narabayashi H, Rausch D, Riederer P, Jellinger K, Nagatsu T (1988) Homospecific activity (activity per enzyme protein) of tyrosine hydroxylase increases in parkinsonian brain. J Neural Transm 72(1):77–82
Mor DE, Tsika E, Mazzulli JR, Gould NS, Kim H, Daniels MJ, Doshi S, Gupta P, Grossman JL, Tan VX, Kalb RG, Caldwell KA, Caldwell GA, Wolfe JH, Ischiropoulos H (2017) Dopamine induces soluble α-synuclein oligomers and nigrostriatal degeneration. Nat Neurosci 20(11):1560–1568
Muñoz P, Huenchuguala S, Paris I, Segura-Aguilar J (2012) Dopamine oxidation and autophagy. Parkinson’s Dis 2012:920953
Nagatsu T, Nakashima A, Ichinose H, Kobayashi K (2019) Human tyrosine hydroxylase in Parkinson’s disease and in related disorders. J Neural Transm 126(4):397–409
Neumann M, Kwong LK, Lee EB, Kremmer E, Flatley A, Xu Y, Forman MS, Troost D, Kretzschmar HA, Trojanowski JQ, Lee VM-Y (2009) Phosphorylation of S409/410 of TDP-43 is a consistent feature in all sporadic and familial forms of TDP-43 proteinopathies. Acta Neuropathol 117(2):137–149
Oberländer U, Pletinckx K, Döhler A, Müller N, Lutz MB, Arzberger T, Riederer P, Gerlach M, Koutsilieri E, Scheller C (2011) Neuromelanin is an immune stimulator for dendritic cells in vitro. BMC Neurosci 12:116
Perez-Riverol Y, Bai J, Bandla C, García-Seisdedos D, Hewapathirana S, Kamatchinathan S, Kundu DJ, Prakash A, Frericks-Zipper A, Eisenacher M, Walzer M, Wang S, Brazma A, Vizcaíno JA (2022) The PRIDE database resources in 2022: a hub for mass spectrometry-based proteomics evidences. Nucleic Acids Res 50(D1):D543–D552
Plum S, Steinbach S, Attems J, Keers S, Riederer P, Gerlach M, May C, Marcus K (2016) Proteomic characterization of neuromelanin granules isolated from human substantia nigra by laser-microdissection. Sci Rep 6:37139
R Foundation for Statistical Computing (2020) R. A language and environment for statistical computing. R Core Team, Vienna
Raper HS (1928) The aerobic oxidases. Physiol Rev 8(2):245–282
Riederer P, Monoranu C, Strobel S, Iordache T, Sian-Hülsmann J (2021) Iron as the concert master in the pathogenic orchestra playing in sporadic Parkinson’s disease. J Neural Transm 128(10):1577–1598
Robert BW, Meghan GT (2012) Moving beyond tyrosine hydroxylase to define dopaminergic neurons for use in cell replacement therapies for Parkinson’s disease. CNSNDDT 11(4):340–349
Sánchez-Ferrer Á, Neptuno Rodríguez-López J, García-Cánovas F, García-Carmona F (1995) Tyrosinase: a comprehensive review of its mechanism. Biochim Biophys Acta 1247(1):1–11
Saper CB, Petito CK (1982) Correspondence of melanin-pigmented neurons in human brain with A1–A14 catecholamine cell groups. Brain : a Journal of Neurology 105(Pt 1):87–101
Sian-Hülsmann J, Mandel S, Youdim MBH, Riederer P (2011) The relevance of iron in the pathogenesis of Parkinson’s disease. J Neurochem 118(6):939–957
Smythies J (1996) On the functional of neuromelanin. Proc R Soc Lond B 263(1369):487–489
Sulzer D, Zecca L (2000) Intraneuronal dopamine-quinone synthesis: a review. Neurotox Res 1(3):181–195
The UniProt Consortium (2021) UniProt: the universal protein knowledgebase in 2021. Nucleic Acids Res 49(D1):D480–D489
Tief K, Schmidt A, Beermann F (1998) New evidence for presence of tyrosinase in substantia nigra, forebrain and midbrain. Mol Brain Res 53(1–2):307–310
Tribl F, Gerlach M, Marcus K, Asan E, Tatschner T, Arzberger T, Meyer HE, Bringmann G, Riederer P (2005) “Subcellular proteomics” of neuromelanin granules isolated from the human brain. MCP 4(7):945–957
Tribl F, Marcus K, Meyer HE, Bringmann G, Gerlach M, Riederer P (2006) Subcellular proteomics reveals neuromelanin granules to be a lysosome-related organelle. J Neural Transm 113(6):741–749
Tribl F, Arzberger T, Riederer P, Gerlach M (2007) Tyrosinase is not detected in human catecholaminergic neurons by immunohistochemistry and Western blot analysis. J Neural Transm. https://doi.org/10.1007/978-3-211-73574-9_8
Tyanova S, Temu T, Cox J (2016a) The MaxQuant computational platform for mass spectrometry-based shotgun proteomics. Nat Protoc 11(12):2301–2319
Tyanova S, Temu T, Sinitcyn P, Carlson A, Hein MY, Geiger T, Mann M, Cox J (2016b) The Perseus computational platform for comprehensive analysis of (prote)omics data. Nat Methods 13(9):731–740
Vila M (2019) Neuromelanin, aging, and neuronal vulnerability in Parkinson’s disease. Mov Disord 34(10):1440–1451
Wheeler JR, Matheny T, Jain S, Abrisch R, Parker R (2016) Distinct stages in stress granule assembly and disassembly. Elife. https://doi.org/10.7554/eLife.18413
Wolozin B (2012) Regulated protein aggregation: stress granules and neurodegeneration. Mol Neurodegener 7:56
Wolozin B, Ivanov P (2019) Stress granules and neurodegeneration. Nat Rev Neurosci 20(11):649–666
Wulf M, Barkovits-Boeddinghaus K, Sommer P, Schork K, Eisenacher M, Riederer P, Gerlach M, Kösters S, Eggers B, Marcus K (2021) Laser microdissection-based protocol for the LC-MS/MS analysis of the proteomic profile of neuromelanin granules. JoVE. https://doi.org/10.3791/63289
Xu Y, Stokes AH, Freeman WM, Kumer SC, Vogt BA, Vrana KE (1997) Tyrosinase mRNA is expressed in human substantia nigra. Brain Res Mol Brain Res 45(1):159–162
Zecca L, Swartz HM (1993) Total and paramagnetic metals in human substantia nigra and its neuromelanin. J Neural Transm Gen Sect 5(3):203–213
Zecca L, Shima T, Stroppolo A, Goj C, Battiston GA, Gerbasi R, Sarna T, Swartz HM (1996) Interaction of neuromelanin and iron in substantia nigra and other areas of human brain. Neuroscience 73(2):407–415
Zecca L, Tampellini D, Gerlach M, Riederer P, Fariello RG, Sulzer D (2001) Substantia nigra neuromelanin: structure, synthesis, and molecular behaviour. Mol Pathol 54(6):414–418
Zhang W, Phillips K, Wielgus AR, Liu J, Albertini A, Zucca FA, Faust R, Qian SY, Miller DS, Chignell CF, Wilson B, Jackson-Lewis V, Przedborski S, Joset D, Loike J, Hong J-S, Sulzer D, Zecca L (2011) Neuromelanin activates microglia and induces degeneration of dopaminergic neurons: implications for progression of Parkinson’s disease. Neurotox Res 19(1):63–72
Zucca FA, Segura-Aguilar J, Ferrari E, Muñoz P, Paris I, Sulzer D, Sarna T, Casella L, Zecca L (2017) Interactions of iron, dopamine and neuromelanin pathways in brain aging and Parkinson’s disease. Prog Neurobiol 155:96–119
Zucca FA, Vanna R, Cupaioli FA, Bellei C, de Palma A, Di Silvestre D, Mauri P, Grassi S, Prinetti A, Casella L, Sulzer D, Zecca L (2018) Neuromelanin organelles are specialized autolysosomes that accumulate undegraded proteins and lipids in aging human brain and are likely involved in Parkinson’s disease. NPJ Parkinson’s Dis 4:17
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The authors would like to thank the team of the Navarrabiomed Brain Bank in Pamplona for providing human post-mortem tissue.
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Open Access funding enabled and organized by Projekt DEAL. This work was supported by de.NBI, a project of the German Federal Ministry of Education and Research (BMBF) (grant number FKZ 031 A 534A) and P.U.R.E. (Protein Research Unit Ruhr within Europe) and Center for Protein Diagnostics (ProDi) grants, both from the Ministry of Innovation, Science and Research of North-Rhine Westphalia, Germany.
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Conceptualization: KM, BE; methodology: MW, BE, KB; software: KS, ME; verification: MW; formal analysis: MW, BE, KS; resources: KM, ME, PR, M.G.; writing—original draft preparation: M.W., K.S., B.E.; writing—review and editing: KB, KM, PR, MG; supervision: KM, BE; project administration: KM; funding acquisition: ME, KM. All authors have read and agreed to the published version of the manuscript.
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Wulf, M., Barkovits, K., Schork, K. et al. Neuromelanin granules of the substantia nigra: proteomic profile provides links to tyrosine hydroxylase, stress granules and lysosomes. J Neural Transm 129, 1257–1270 (2022). https://doi.org/10.1007/s00702-022-02530-4
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DOI: https://doi.org/10.1007/s00702-022-02530-4