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Fabrication of microchip based on UV transparent polymer for DNA electrophoresis by F2 laser ablation

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Abstract

A microchip for DNA electrophoresis made of a new UV transparent polymer material (CYTOP) whose absorption edge is much shorter than 190 nm has been fabricated by F2 laser ablation. F2 laser ablation achieves high-quality microfabrication of CYTOP surface with little deterioration of the optical property and little debris deposition at etched area. The microchip in which the microchannel connected with two reservoirs was embedded was successfully fabricated by bonding the ablated sample to the virgin CYTOP sheet. The fabricated microchip was applied for DNA analysis by electrophoresis and succeeded in separating different base-pares (bp) of DNA from 50 to 766 bp with resolution of 100 bp.

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Authors and Affiliations

  1. RIKEN (The Institute of Physical and Chemical Research), Hirosawa 2-1, Wako, Saitama, 351-0198, Japan

    K. Obata, K. Sugioka & K. Midorikawa

  2. Brain Science Institute (BSI), RIKEN, Hirosawa 2-1, Wako, Saitama, 351-0198, Japan

    N. Shimazawa

Authors
  1. K. Obata
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  2. K. Sugioka
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  3. N. Shimazawa
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  4. K. Midorikawa
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Correspondence to K. Obata.

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PACS

42.70.Jk; 52.38.Mf; 82.45.-h

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Obata, K., Sugioka, K., Shimazawa, N. et al. Fabrication of microchip based on UV transparent polymer for DNA electrophoresis by F2 laser ablation. Appl. Phys. A 84, 251–255 (2006). https://doi.org/10.1007/s00339-006-3618-9

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  • DOI: https://doi.org/10.1007/s00339-006-3618-9

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