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Endophytic Fungus Talaromyces sp. MR1 Promotes the Growth and Cadmium Uptake of Arabidopsis thaliana L. Under Cadmium Stress

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Abstract

Endophytes play essential roles in plant growth under metal(loid)s stress. An endophytic fungus strain MR1 was isolated from the roots of Miscanthus floridulus collected from a lead–zinc mining area (Huayuan, China), which could produce indole-3-acetic acid and have Cadmium (Cd) tolerance. Further 18S rRNA sequencing analysis showed that it was highly similar (99.83%) to Talaromyces pinophilus. In pot experiments, we explored the effects of strain MR1 on the growth and Cd uptake of a wide-type Arabidopsis thaliana under low (LC) and high (HC) Cd concentrations. The results showed that MR1 effectively increased the dry weight of aboveground and underground tissues by 25.95–107.21% in both LC and HC groups. Due to MR1 inoculation, the Cd content in the underground tissues was significantly (p < 0.05) decreased by 39.28% under low Cd concentration, while it was significantly (p < 0.05) increased by 28.28% under high Cd concentration. Besides, MR1 inoculations significantly (p < 0.05) increased the total content of removed Cd (17.080 μg) and BCF (0.064) by 129.77% and 153.95% under high Cd concentration. Therefore, we speculated that MR1 might be selected as the effective microbial agent to increase crop yield and control Cd content in the crop in light Cd-contaminated soil. Besides, MR1 could potentially enhance the phytoremediation efficiency of extremely Cd-contaminated soil.

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Data Availability

The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.

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Acknowledgements

This project was carried out with great support from Professor Langtao **ao and Chao Huang’s research Lab. The study was supported by the National Natural Science Foundation of China (No. 41807135), the Scientific Research Fund of Hunan Provincial Education Department (No. 21A0142, 20A233, 18B107), and the Natural Science Foundation of Hunan province, China (No. 2019JJ50220).

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Authors and Affiliations

  1. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China

    Yunhua **ao, Rui Chen, Liang Chen, Bo Yang, Lihong Jiang & Jun Fang

  2. Hunan Engineering Laboratory for Pollution Control and Waste, Utilization in Swine Production, Changsha, 410128, China

    Yunhua **ao, Bo Yang, Lihong Jiang & Jun Fang

  3. Key Laboratory for Rural Ecosystem Health in Dongting Lake Area of Hunan Province, Changsha, 410128, China

    Yunhua **ao, Bo Yang, Lihong Jiang & Jun Fang

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  1. Yunhua **ao
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Contributions

YX, JF, and LJ contributed to the study’s conception and design. Research, material preparation, data collection, and analysis were performed by RC, LC, and YX. The first draft of the manuscript was written by RC, and YX and BY commented on previous versions of the manuscript. All aforementioned authors read and approved the final manuscript.

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Correspondence to Lihong Jiang or Jun Fang.

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**ao, Y., Chen, R., Chen, L. et al. Endophytic Fungus Talaromyces sp. MR1 Promotes the Growth and Cadmium Uptake of Arabidopsis thaliana L. Under Cadmium Stress. Curr Microbiol 80, 346 (2023). https://doi.org/10.1007/s00284-023-03453-3

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