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Isolation and Characterization of the Lytic Cold-Active Bacteriophage MYSP06 from the Mingyong Glacier in China

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Abstract

As unique ecological systems, glaciers are characterized by low temperatures and low nutrient levels, which allow them to be considered as “living fossils” for the purpose of researching the evolution of life and the environmental evolution of the earth. Glaciers are also natural microbial “reservoirs”. In this work, a lytic cold-active bacteriophage designated MYSP06 was isolated from Janthinobacterium sp. MYB06 from the Mingyong Glacier in China, and its major characteristics were determined. Electron microscopy revealed that bacteriophage MYSP06 had an isometric head (74 nm) and a long tail (10 nm in width, 210 nm in length). It was classified as a Siphoviridae with an approximate genome size of 65–70 kb. A one-step growth curve revealed that the latent and burst periods were 95 and 65 min, respectively, with an average burst size of 16 bacteriophage particles per infected cell. The bacteriophage particles (100 %) adsorbed to the host cells within 10 min after infection. Moreover, the pH value and thermal stability of bacteriophage MYSP06 were also investigated. The maximum stability of the bacteriophage was observed at the optimal pH 7.0, and the bacteriophage became completely unstable at the extremely alkaline pH 11.0; however, it was comparatively stable at the acidic alkaline pH 6.0. As MYSP06 is a cold-active bacteriophage with a lower production temperature, its characterization and its relationship with its host Janthinobacterium sp. MYB06 deserve further study.

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Acknowledgments

This study was supported by grants from the Scientific Research Program of the Higher Education Institution of **nJiang (Grant Number XJEDU2014S052), the Natural Science Foundation of China (30960079/31160121), and the China Scholarship Council. We are grateful to American Journal Experts (AJE) for critical reading of the manuscript.

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Authors and Affiliations

  1. Key Laboratory of Ecology and Biological Resources in Yarkand Oasis, Kashgar University, Kashi, 844006, **njiang, China

    Mingyuan Li & Jilian Wang

  2. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650091, Yunnan, China

    Mingyuan Li, Qi Zhang, Lianbing Lin, **uling Ji & Yunlin Wei

  3. Department of Biology, The University of Texas-Pan American, Edinburg, TX, 78539, USA

    Anxin Kuang & Luis Alberto Materon

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  1. Mingyuan Li
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  2. Jilian Wang
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  3. Qi Zhang
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  7. **uling Ji
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Correspondence to **uling Ji or Yunlin Wei.

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Li, M., Wang, J., Zhang, Q. et al. Isolation and Characterization of the Lytic Cold-Active Bacteriophage MYSP06 from the Mingyong Glacier in China. Curr Microbiol 72, 120–127 (2016). https://doi.org/10.1007/s00284-015-0926-3

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  • DOI: https://doi.org/10.1007/s00284-015-0926-3

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