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Effects of spaceflight on polysaccharides of Saccharomyces cerevisiae cell wall

  • Applied Microbial and Cell Physiology
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Abstract

Freeze-dried samples of four Saccharomyces cerevisiae strains, namely, FL01, FL03, 2.0016, and 2.1424, were subjected to spaceflight. After the satellite’s landing on Earth, the samples were recovered and changes in yeast cell wall were analyzed. Spaceflight strains of all S. cerevisiae strains showed significant changes in cell wall thickness (P < 0.05). One mutant of S. cerevisiae 2.0016 with increased biomass, cell wall thickness, and cell wall glucan was isolated (P < 0.05). The spaceflight mutant of S. cerevisiae 2.0016 showed 46.7%, 62.6%, and 146.0% increment in biomass, cell wall thickness and β-glucan content, respectively, when compared to the ground strain. Moreover, growth curve analysis showed spaceflight S. cerevisiae 2.0016 had a faster growth rate, shorter lag phase periods, higher final biomass, and higher content of β-glucan. Genetic stability analysis showed that prolonged subculturing of spaceflight strain S. cerevisiae 2.0016 did not lead to the appearance of variants, indicating that the genetic stability of S. cerevisiae 2.0016 mutant could be sufficient for its exploitation of β-glucan production.

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Acknowledgements

This research was funded by the Critical Technique for Food Safety, National Key Technologies R&D Program, Chinese Center for Disease Control and Prevention (2006BAK02A07-2) and supported by the Outstanding Researcher’s Fund in the Chinese Academy of Agriculture Sciences. We would like to thank Lu-**ang Liu and Jia-** Lv for their help in the spaceflight.

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Authors and Affiliations

  1. Institute of Agro-food Science and Technology, Chinese Academy of Agriculture Sciences, Bei**g, 100193, People’s Republic of China

    Hong-Zhi Liu, Qiang Wang & **ao-Yong Liu

  2. School of Chemical and Life Sciences, Singapore Polytechnic, 500 Dover Road, Singapore, 139651, Singapore

    Sze-Sze Tan

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  1. Hong-Zhi Liu
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  2. Qiang Wang
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  3. **ao-Yong Liu
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  4. Sze-Sze Tan
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Correspondence to Qiang Wang.

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Liu, HZ., Wang, Q., Liu, XY. et al. Effects of spaceflight on polysaccharides of Saccharomyces cerevisiae cell wall. Appl Microbiol Biotechnol 81, 543–550 (2008). https://doi.org/10.1007/s00253-008-1692-y

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  • DOI: https://doi.org/10.1007/s00253-008-1692-y

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