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Application of cloud point extraction for residues of chloramphenicol and amoxicillin in milk samples by HPLC–DAD

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Abstract

A pre-concentration and sensitive determination method for residues of Chloramphenicol (CLP) and Amoxicillin (AMX) in milk samples were developed based on cloud point extraction (CPE) and HPLC–DAD analysis. CLP and AMX molecules were extracted to surfactant phases of polyethylene glycol-6000 (PEG-6000) in the presence of pH 7.0 buffer and high electrolyte concentration. Experimental variables were examined and optimized such as concentration of electrolyte and surfactant, pH, incubation time, and diluting solvent. In the developed method, determination of CLP and AMX using HPLC system was carried out by isocratic elution of water:acetonitrile (30:70) mixture. CLP and AMX antibiotic molecules were analyzed by considering peak area obtained by DAD detector at 273 nm and 276 nm, respectively. After optimization experimental variables, analytical signals were linear in the range of 10–900 ng mL−1 and 25–1000 ng mL−1, respectively. The limit of detection values was calculated as 2.98 and 7.46 ng mL−1 while relative standard deviations (RSD %) were lower than 4.20% for 100 ng mL−1. Finally, the developed method was successively applied to cow milk samples.

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Funding

This study has been supported by Sivas Cumhuriyet University Scientific Research Projects Commission as the research project with the ECZ-048 code.

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Authors and Affiliations

  1. Department of Pharmaceutical Technology, Faculty of Pharmacy, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey

    Bilge Sürücü

  2. Department of Analytical Chemistry, Faculty of Pharmacy, Sivas Cumhuriyet University, Sivas, Turkey

    Bilge Sürücü, Halil Ibrahim Ulusoy, Özge Demir & Sümeyra Gülle

  3. Department of Pharmacy, Vocational School of Health Service, Sivas Cumhuriyet University, Sivas, Turkey

    Songül Ulusoy

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  1. Bilge Sürücü
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  2. Halil Ibrahim Ulusoy
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  3. Songül Ulusoy
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  5. Sümeyra Gülle
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Contributions

BS: methodology, formal analysis, investigation, visualization, and data curation. HİU: writing—original draft, methodology, formal analysis, investigation, visualization, and data curation. SU: investigation, methodology, and formal analysis. SG: conceptualization and validation. ÖD: writing—review and editing.

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Correspondence to Halil Ibrahim Ulusoy.

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Sürücü declares that there is no conflict of interest. Halil İbrahim Ulusoy declares that there is no conflict of interest. Songül Ulusoy declares that there is no conflict of interest. Özge Demir declares that there is no conflict of interest. Sümeyra Gülle declares that there is no conflict of interest.

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Sürücü, B., Ulusoy, H.I., Ulusoy, S. et al. Application of cloud point extraction for residues of chloramphenicol and amoxicillin in milk samples by HPLC–DAD. Eur Food Res Technol 248, 437–445 (2022). https://doi.org/10.1007/s00217-021-03889-6

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  • DOI: https://doi.org/10.1007/s00217-021-03889-6

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