Introduction

Foodborne diseases caused by the consumption of contaminated food and water are a major public health burden, seriously threatening people's health and life, and hindering economic development worldwide [7, 8]. Immunological detection methods are characterized by high specificity due to the specific interaction between antibodies and antigens on the surface of the target bacteria. However, the high cost, poor stability, and limited sources of antibodies hinder the further application of immunoassay techniques [9]. Therefore, it is necessary to establish a rapid, simple, sensitive, and reproducible field detection method for evaluating food safety.

In recent years, microfluidics technology has provided powerful tools for detection applications with its portability, miniaturization, automation, multichannel sample detection, minimal treatment of harmful substances, and cost savings [10,11,12]. The greatest advantage of microfluidics over traditional methods is the creation of a controlled microenvironment that precisely drives and controls microfluidic flow in microchannels, improving detection sensitivity [13]. In addition, all analytical processes, including sample preparation, reaction, separation, and detection, are integrated into a single microfluidic chip for field testing applications [11]. Biosensors using various technologies combined with microfluidic chips for the detection of foodborne pathogens have been widely reported [50]. Thus, this newly developed dual-RCA whole-cell assay is a promising method for rapid and sensitive detection of foodborne bacteria using microfluidic devices.

Fig. 3
figure 3

Schematic drawing of a dual-RCA E. coli O157:H7 whole-cell detection system. (a) Photograph of a completely packaged microfluidic detection device. (b) Schematic illustration of sandwich detection format, in which the surface of the microfluidic channel is modified with PAMAM dendrimers, followed by cRCA in situ to produce repeated aptamers to capture target cells. Reproduced from [50] with permission of Elsevier

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Aptamers have been tested for their multiplexing ability to simultaneously identify a variety of bacteria in real food samples, indicating their potential application in microfluidic systems. Wang et al. [46] first screened bacteria-specific aptamers with SELEX and integrated them into microfluidic systems. First, a biotin-labeled aptamer was bound to the nitrocellulose membrane in the chip, followed by incubation with bacteria, and finally, tetramethylbenzidine-streptavidin (blue) chromogenic reaction was utilized to simultaneously identify three target bacteria. This new microfluidic chip has advantages such as fast detection time (35 min), small size, high specificity, and simultaneous detection of multiple pathogens. Based on the development of nucleic acid aptamer microfluidic detection of pathogenic bacteria mentioned above, nucleic acid aptamer has some limitations, including fast nuclease degradation and short action time. In many cases, the sensitivity and selectivity of aptamer assays are affected by sample conditions, such as interfering components (i.e. proteins, lipids, carbohydrates), pH, and ionic strength. In addition, nonspecific binding of aptamers to sample components may lead to false-positive results. Therefore, the samples must be pretreated before detection. A common method is magnetic separation using magnetic beads or magnetic nanoparticles, which can significantly improve the sensitivity. These limitations affect the development of efficient and sensitive microfluidic detection devices and require further research.

Carbohydrate-binding protein

Compared with other bio-recognition elements, lectins are the most commonly used due to their broad spectrum of pathogen capture capability. Lectins can detect carbohydrates on bacterial surfaces. Interestingly, because of the adhesion mechanism between lectins and bacteria, carbohydrates can also recognize lectins contained in bacterial structures [51]. Mannose-binding lectins (MBL) are considered the first defense mechanism of the host and are expressed on the surface of more than 90 different viruses, bacteria, and fungi by binding to terminal mannose and fucose residues [52]. This feature has inspired researchers to combine MBL on microfluidic devices to capture foodborne pathogens. Cooper et al. [53] developed a microfluidic device coated with modified MBL on magnetic beads for the detection of various pathogens. The device can detect Candida albicans cells in human blood at a concentration of 1 cell/mL within 3 h. Similar methods are employed to capture S. aureus from plasma and blood with a detection limit of 102 CFU/mL [54]. Concanavalin A (ConA) is also a typical lectin commonly used to enrich pathogenic bacteria. A highly sensitive nanobiosensor based on isothermal amplification and microfluidic enrichment was developed using a microfluidic chip immobilized with ConA as an enrichment probe to enrich pathogenic bacteria and extract genomic DNA from isolated bacteria. Under optimal conditions, S. typhimurium at a concentration of 5 CFU/mL could be detected in 10 mL urine samples in real time by a label-free method. In addition, the whole experiment was completed within 100 min [55]. This sensor is a simple, rapid, and sensitive diagnostic platform. Importantly, this method can be used for the detection of urinary tract infection caused by Salmonella or other pathogenic bacteria.

Overall, although lectins have rarely been used in microfluidic platforms and have been less studied for foodborne pathogen detection, the above examples demonstrate their potential in this regard. Furthermore, due to the unique nature of the interaction between lectins and glycoproteins, the resulting vectors can be easily regenerated. Lectins may be used in combination with other simple bio-recognition elements in the future.

Phages

Bacteriophages have attracted extensive attention due to their specificity and sensitivity to targeted microorganisms. They can be turned into a potential foodborne pathogen bio-recognition element that can remain active under various extreme conditions (such as high temperature, organic solvents), and are a potential candidate for the development of biosensors for bacterial detection [56]. Phages usually consist of two distinct parts, the head containing phage DNA and the tail responsible for bacterial recognition. Since there are many different types of phages, with many shapes and characteristics, the designed biosensor can detect almost all bacterial strains [57]. Unlike other bio-recognition elements, phages can be easily and inexpensively produced in large quantities. Generally, a large number of progeny phages can be obtained by simply infecting bacterial solutions. These advantages make phages a popular bio-recognition element in biosensors [58]. A novel label-free electrochemiluminescence (ECL) biosensor for the detection of P. aeruginosa was prepared by immobilizing PaP1 phages on carboxyl graphene with a detection limit as low as 56 CFU/mL [59].

Despite the many advantages of phages, however, they lose activity if they are fixed to a sensor surface and have dried out. In addition, because of the relatively large size of the phage, using whole phages as the recognition element leads to a decrease in the measurement signal. Therefore, instead of using whole phages, it is better to only use phage-derived proteins for detection. Endolysin is one of these proteins and is produced at the end of phage lysis. Endolysin is immobilized on the cell wall by carbohydrate-binding domains (CBD), particularly targeting the bacterial peptidoglycan layer [60]. Because CBD is smaller in size than antibodies and there are more CBD binding sites in the bacterial cell walls, CBD can be used as a bacterial capture recognition element in microfluidic devices. Yi et al. [61] isolated S. aureus cells from sample matrix with lysine CBD-functionalized beads, and used fluorescence detection for rapid (50 min), specific, and sensitive quantitative analysis of S. aureus in real samples. In phosphate-buffered saline (PBS), the detection limit was as low as 78 CFU/mL for S. aureus in a concentration range from 1.0 × 102 to 1.0 × 107 CFU/mL. However, CBD was unable to recognize and adhere to Gram-negative bacteria, mainly because the outer membrane of these bacteria shields peptidoglycans. However, the above reports show the potential for further exploring the integration of bacteriophage CBD into microfluidic chips to develop a sensitive detection device for foodborne pathogenic bacteria.

Molecularly imprinted polymers (MIPs)

The biometric elements listed above are natural biomolecules, such as proteins, peptides, and sugars. Although the applications of these bio-recognition elements in biosensors are relatively mature, their high cost, poor stability, complex preparation, and non-reusability have limited their further development in biosensors. MIPs are a kind of artificially prepared recognition materials; the preparation process is simple and easy [62]. Compared with natural bio-recognition elements, the outstanding advantages of MIPs include on-demand preparation and reversible adsorption/release of target molecules [38]. In addition, MIPs can recognize not only small molecules, but also various macromolecular targets, including proteins [63], viruses [64], and microorganisms [65]. For example, S. aureus was isolated from complex food samples by molecular imprinting. Dopamine was used as a functional monomer, magnetic MIPs by imprinting on the surface of magnetic particles, and fluorescence microscopy was used for detection. Subsequently, MIPs were used to extract S. aureus from milk and rice, and bacteria in milk could be detected at a concentration of 1 × 103 CFU/mL [66].

To further improve the detection sensitivity of low-abundance targets, in recent years MIPs have been immobilized on sensors for enrichment, separation, and detection of low-abundance target molecules. Bezdekova et al. [67] combined fluorescent QDs with MIPs on a 3D origami paper-based microfluidic device for specific recognition and sensitive fluorescence detection of phycocyanin. QDs are typically small fluorescent nanocrystals that have been widely used for sensor development and enable ultrasensitive detection of target molecules due to their unique optical and photochemical properties. Furthermore, MIPs were used in this study because of their reusability and good specificity and selectivity. Importantly, the device can provide quantitative information very conveniently, and can be further expanded and applied to the detection of other proteins or biomarkers in environmental and food safety studies. Although there are few examples of the use of MIPs in microfluidic devices for the capture and detection of foodborne pathogens, the facile process and broad-spectrum potential will be illustrated in other future examples.

Application of microfluidic chips in sample pretreatment of foodborne pathogens

It is difficult to detect pathogens found in untreated food samples without proper sample pretreatment steps. The concentration of many pathogens in food samples is very low. Additionally, the variety and content of other substances in complex food matrices may interfere with detection and reduce its accuracy. Therefore, enrichment and separation of target analytes are important for detection purposes. Accordingly, many microfluidic platforms have been adapted to the complexity of sample preparation and enrichment [68]. Typically, the duration of food analysis work is 2–5 days, while microfluidic technology can reduce the analysis time to several hours. For example, a modular integrated microfluidic chip can be utilized to rapidly and efficiently prepare foodborne pathogen screening samples. It employs an integrated microheater for thermal cleavage and DNA amplification on the chip, reaching the temperatures required for DNA amplification (65 °C) and lysis steps (95 °C) in 25 s and 60 s, respectively [69]. The proposed bacterial sample preparation LOC platform has all the required performance criteria and holds promise for its wide applicability to bacterial identification in food, clinical, and water samples.

When microfluidic chips are used directly to detect pathogens in complex matrices, the reproducibility of the method is poor because the concentration of pathogenic bacteria to be detected is too low. Therefore, it is necessary to isolate and enrich analytes from the food matrix to improve the efficiency of target detection. Because of the high specific surface area of magnetic particles, they are widely used in microfluidic devices to increase pathogen bio-recognition. Pathogen-specific RNA of the Phalaenopsis orchid was pretreated with magnetic beads, followed by reverse transcription loop-mediated isothermal amplification (RT-LAMP) to amplify pathogen RNA. The results demonstrated that the system could complete detection in 65 min with a detection limit of 25 fg [70]. To carry out the continuous pretreatment operation of biological detection reagents, a microfluidic bioseparation chip integrating mixer, heater, and soft magnet were proposed for nucleic acid detection and magnetic bead extraction of target substances [71]. Recently, Guan et al. [72] successfully prepared a novel magnetically driven droplet microfluidic immunosensor without a complex operating system for the enrichment of cyanobacterial toxins in aquatic products. Compared with widely used microfluidic systems, the proposed sensor has two significant advantages: simple and effective operation by magnetic force alone rather than inconvenient actuation devices, and high-throughput parallel detection on a 15-channel chip.

IMS can be used to concentrate bacterial cells at lower concentrations, but it is only suitable for small-volume samples (1 mL), which limits the enrichment of large-volume samples. To solve this problem, Ngamsom et al. [73] combined magnetic electrophoresis and IMS for the rapid enrichment, sorting, and detection of live S. typhimurium and E. coli O157:H7 in food samples. The platform utilizes two different types of specific magnetic beads. According to the different size and number of magnetic beads, they can specifically capture and concentrate live S. typhimurium and E. coli O157:H7 by applying a nonuniform magnetic field perpendicular to the flow direction, and the beads deviate from the flow direction in the chip to different degrees. Thus, the combination of magnetic electrophoresis and IMS may be the future direction for generating high-throughput concentrations of target pathogens from complex food samples.

In addition to microfluidic devices integrated with magnetic beads, filters and membranes are another direct and cost-effective alternative for rapid concentration of target pathogens, especially in large-volume samples. Li et al. [

Biosensor-based microfluidics for the detection of foodborne pathogenic bacteria

The development of biosensors integrates knowledge from biology, chemistry, physics, medicine, and electronics. Biosensors are sensitive to biological substances and can convert signals such as the concentration and activity of an analyte into electrical and optical signals for rapid detection [80, 81]. Microfluidic technology can reduce pretreatment steps because of its specificity, sensitivity, rapidity, simplicity of equipment, and low skill requirements for operators [82, 83]. Combining sample pretreatment and analysis of foodborne pathogens into a microfluidic chip can help achieve high efficiency, high speed, and automation [84].

Microfluidic chip with optical biosensor detection methods

The optical biosensor has strong anti-interference ability, miniaturized equipment, convenient operation, telemetry, real-time, on-line and dynamic monitoring, fast response, and high sensitivity [85]. The detection system integrated with an optical biosensor and microfluidic chip can add, separate, prepare, and analyze samples on a single device. It has the advantages of low cost, short detection time, multi-throughput, automation, portability, versatility, and low sample quantity [64].

Colorimetric biosensor

The colorimetric biosensor is a very attractive optical biosensor system because one can easily observe the pathogenic microorganisms in the sample with the naked eye by changing the color of the reaction solution without any analytical instrument. The combination of colorimetric analysis and microfluidic chip technology has been widely used in the detection of pathogenic bacteria [26, 86]. Among the wide range of microfluidic chip detection technologies, paper-based material has unique advantages, as the high specific surface area and volume increase the detection limit of colorimetry. The material is suitable for develo** diagnostic tools that are reasonably priced, portable, disposable, and easy to detect and handle.[87] Sun et al. [26] developed a paper-based analytical device (μ-PAD) by combining PVC pad with filter paper. The detection is achieved by measuring the color changes from colorless to indigo for the specific species of enzymes associated with Cronobacter spp. The substance of interest reacts with the chromogenic substrate. Because of the optimization of the specific enrichment process, living bacteria measured as low as 10 CFU/cm2 on the inoculated surface of the sample can be detected. This paper-based analysis device can also be combined with an IMS phase to detect S. typhimurium. The lowest detection limit for S. typhimurium in the culture medium is 10 CFU/mL. This detection system was applied to starling bird fecal samples and whole milk with detection limits of 10 CFU/g and 10 CFU/mL, without any pre-enrichment and with high reproducibility [88]. The μPAD-colorimetric detection system developed by Jokerst et al. [27] uses filter paper wax printing technology that is realized by measuring the color change of the enzyme associated with the pathogen of interest when it reacts with the chromogenic substrate. When combined with the enrichment process, the method allows for enrichment time of 12 h or less, and the concentration in inoculated ready-to-eat meat can be detected at levels as low as 10 CFU/mL. The main drawbacks of colorimetric detection are low selectivity, low sensitivity, and long detection time. Combining the paper microdot test with the colorimetric method can provide a simple, economical, and less time-consuming method for detecting pathogens.

Fluorescence biosensor

As an important branch of optical biosensors, fluorescence biosensors have advantages including high sensitivity and easy readability [88,89,90]. Fluorescence detection is more sensitive than colorimetric detection. New methods and techniques for quantitative detection of bacteria chips are constructed by combining a microfluidic chip analysis system with fluorescence detection technology [91], which has become a potentially powerful tool and research hotspot in the field of bacterial analysis and detection due to its high efficiency and specificity in detecting bacteria [92]. Wang and colleagues used CdSe/ZnS@SiO2-NH2 composite nanoparticles (FNPs) as a fluorescent label for the detection of S. typhimurium. Hydrophobic CdSe/ZnS QDs were introduced into SiO2 microspheres by self-modified inverse microemulsion technology. FNPs were coupled with bacteria by a glutaraldehyde two-step method. The detection limit was 3.3 × 102 CFU/mL. To further reduce the detection limit and obtain better visualization, an integrated dielectrophoresis (DEP) microfluidic chip and related microsystem were created. Positive DEP was used to enrich FNP-labeled bacteria along the edge of the microchannel cross microelectrode by positive electrophoresis and counted under a fluorescence microscope [28]. Zhang et al. [93] designed a simple, rapid, fixed-point magnetic immunofluorescence detection method for avian influenza virus (AIV), enabling the integration of immunomagnetic target capture, concentration, and fluorescence detection in the microfluidic chip. By optimizing the flow rate and incubation time, the detection limit was 3.7 × 104 copy/μL, the injection volume was 2 μL, and the total detection time was less than 55 min. The method is characterized by good portability, fast analysis, high specificity, high precision, and good reproducibility. This microfluidic system can provide a powerful platform for rapid detection of AIV and may be expanded to detect other viruses and pathogens (Fig. 4A). This convenient experimental device enables the development of a real-time diagnostic system while maintaining appropriate sensitivity. Kubol et al. [29] developed a method for detecting intestinal Salmonella by PCR using fluorescent probes on microfluidic disc devices. The cells of Salmonella enterica were isolated in the microchamber of the apparatus and then thermally lysed, and the polymerase chain reaction targeting the invA gene specific to S. enterica was observed by measuring the fluorescence signal generated by gene amplification. This method can detect living cells without inhibition by any egg component. Salmonella enterica was detected in egg yolks at 5.0 × 104 cells/mL or higher concentrations within 6 h including sampling time. The use of functionalized MNPs to capture and enrich bacteria has aroused the interest of many researchers in the field. Microfluidic and MNPs combined with effective capture antibody immobilization, as well as a fluorescence detection system, enabled the detection of L. monocytogenes at a detection limit of 10 CFU/mL [94]. The system integration can better show the design and application concept of lab-on-a-chip (LOC), indicating the direction of research and development of microfluidic microchip bacterial analysis systems and instruments.

Fig. 4
figure 4

(A) Schematic diagram of the combination of fluorescence and microfluidic detection device. Reproduced from [93] with permission of the American Chemical Society. (B) Schematic of a quantitative portable plasma platform integrated with microfluidic for pathogen detection and quantification. (a) The surface activated disposable microfluidic chips were mounted on the top side of the device. (b) The electronic setup of the device is represented from bottom. (c) Schematics of the microfluidic integrated SPR platform. Reproduced from [32] with permission of Springer Nature. (C) The working principle of the microfluidic device for bacteria enrichment and SERS analysis. (a) Side view, (b) cross-section, (c) the overall appearance of the LOC device for the concentration of bacteria followed by SERS detection. Reproduced from [118] with permission of John Wiley and Sons

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CL biosensor

The CL technique has several favorable features including fast reaction and high sensitivity, as well as perfect measurement instruments and good supply of reagents. CL is widely used for the determination of hormones and IgG, and has received increasing attention in microbiological research [95]. Generally, the CL method is used for determining the number of microorganisms and has been widely applied in the detection of bacteria [96, 97]. Dong et al. [30] developed an organic hybrid heterojunction photodiode (OPD) array integrated with a multi-cavity microfluidic biosensor for the detection of multiple pathogens. The composite microfluidic biosensor made of polymethylenoate (PMMA) was integrated into the OPD array for the CL detection of pathogens. The CL detection limit of E. coli is 5 × 105 cells/mL and E. jejuni is 1 × 105 cells/mL. The detection system uses simple spin coating and injection molding to manufacture the OPD array and PMMA chip, and thus can realize the mass production of an integrated optical biosensor platform. When analyzing and detecting bacteria in real samples, microfiltration and/or IMS is usually incorporated into the integrated optical biosensor platform to realize the micro total analysis system. While the full analysis system has advantages, pathogen pre-concentration on the chip will lead to longer analysis time and higher chip cost. On the contrary, the design and manufacture of the CL platform will encourage its use as a disposable system. After the large-scale production of OPD arrays and microfluidic chips, the analysis cost of the platform is greatly reduced.

Electrochemiluminescence (ECL) is a method for initiating and controlling CL reactions by applying electrochemical potentials, and has become increasingly important in analytical chemistry in recent years. Delaney et al. [98] first developed a method to combine paper-based microfluidic technology with ECL detection. Inkjet printing is used to produce paper microfluidic substrates. The substrate was combined with screen-printed electrodes (SPEs) to form a simple, cheap, and disposable sensor that could be read without a traditional photodetector. 2-(Dibutylamino)-ethanol (DBAE) and nicotinamide adenine dinucleotide (NADH) could be detected to levels of 0.9 μM and 72 μM, respectively. It is worth noting that mobile camera phones can be used to detect the glow emitted by sensors. A calibration curve was constructed by analyzing the intensity of red pixels in the digital images of ECL emission, showing that DBAE could be detected to levels of 250 μM using the mobile phone. Park and Yoon [31] used paper microfluidic technology to successfully detect E. coli in field water samples with smartphones. They designed a three-channel paper with a negative control channel preloaded with bovine serum albumin (BSA)-conjugated beads and two E. coli detection channels preloaded with anti-E. coli-conjugated beads for low- or high-concentration detection. This paper-based microfluidic device combines the smartphone with its internal gyroscope to allow users to position the smartphone at the best scattering detection angle. The lower limit is single-cell level, and the total detection time is 90 s. The device does not require any external hardware except paper chips, smartphones with built-in gyro sensors, and installed software applications. It provides a good example of rapid detection of pathogenic bacteria. Researchers not only improve the convenience of microfluidic in intelligence, but also reduce the cost of biosensor material selections to develop simpler and cheaper biosensors. Shang et al. [99] proposed a cloth-based CL biosensor for the first time to detect the hlyA gene of L. monocytogenes with high sensitivity. The CL biosensor has good analytical performance, such as high detection ability and specificity, wide linear range, and acceptable reproducibility and stability. Finally, the proposed biosensor has been proven to successfully detect target DNA prepared from milk samples supplemented with L. monocytogenes. Therefore, it is considered to have application potential in food safety and environmental monitoring.

Surface plasmon resonance (SPR) biosensor

SPR is generated by normal-mode light excitation of electron density fluctuations at the interface between two different media with opposite relative tolerance, for example, at the interface of a metal and a dielectric material. SPR-based microfluidic technology has advantages including automation, small size, and fast processing speed [100]. It is possible to combine methods by proper design and thereby improve sensing efficiency. Because samples react simultaneously with multiple bio-recognition elements immobilized on a single chip, the microfluidic can help increase the throughput of a single chip to provide better sample stream delivery [101]. Commercial SPR sensors have been widely employed to detect E. coli, S. enterica, S. typhimurium, and L. monocytogenes in food samples [102]. Lee et al. [100] used optical fiber SPR sensors and PCR chips integrated into a compact form for the production and detection of Salmonella DNA amplification products. The SPR optical fiber sensor was made of multimode optical fiber with bimetallic SPR coating, while the PCR chip was composed of PMMA substrates in the form of microchannels. Although the integrated device does not need fluorescence labeling-related parameters, including background signal and sample size, it can detect the refractive index change around the sensor surface caused by the injected DNA amplifier, as long as the DNA sample is close to the sensor surface. The unlabeled characteristic of the device can ensure its reproducibility. At present, the device is being further miniaturized to facilitate POCT [105]. Morlay et al. [106] developed an approach involving the detection of live bacteria during their growth phase via the use of an immuno-chip SPR imaging process. The sensor can detect very small amounts of L. monocytogenes in food samples in a day. This method for real-time detection of L. monocytogenes showed remarkable performance in terms of sensitivity and specificity. A gold biochip was functionalized with seven different polyclonal antibodies. All the antibodies could successfully bind to bacterial cells. This method can detect L. monocytogenes within 30 min. Compared with the current methods, the method reduces the time of processing and producing results, but it still takes 24 h of enrichment steps. Recently, a custom SPRI sensor was connected to a smartphone for antibody detection. Guner and colleagues developed a low-cost grating coupled SPR sensor chip using off-the-shelf optical storage discs [107]. The transmission surface plasmon resonance (TSPR) measurement offers outstanding advantages over traditional SPR techniques. Since the TSPR optical signal does not have optical noise derived from the reflected light, the unique TSPR peak and the main bright spots on the dark background indicate transmitted light enhancement and improved signal-to-noise ratio. A strong and narrow TSPR excitation peak covering the visible near-infrared region (900 nm) was obtained on the gold-plated grating substrate [108]. This work makes use of special transmission signals from the plasma grating structure, which has a microfluidic system called microfluidic transmission surface plasmon resonance (MTSPR). The technology has benefits including simple operation, strong flexibility, good repeatability, and great possibility for further commercialization. Lertvachirapaiboon and colleagues applied MTSPR fabricated by a gold-plated grating substrate and microchannel to the biosensor for the detection of glucose. The detection limit of the developed system for glucose was 2.31 mM. This practical platform represents high potential for the further development of several biomolecules, multiple systems, and POC analysis for practical biosensor applications [109]. Although this MTSPR platform is not designed for bacterial detection, its miniaturized design is suitable for POC applications that require disposable SPR sensors. This typical sensor chip provides a simple program and practical system for the application of biosensors. In addition, the possibility of further develo** sensor chips for multiple detection, POC detection, and portable biosensor devices is high. SPR sensors are based on localized surface plasmon resonance (LSPR) of metal nanostructures with surface plasmon modes at the structural interface. The LSPR sensor with various nanostructures is a miniature version of the SPR sensor, and requires neither a prism nor a complex optical system, but a simple transmittance measurement [110]. Therefore, the SPR-microfluidic chip analysis platform will appear at the forefront of POCT, promote new ideas and changes to modern laboratory medicine, and highlight the characteristics of simplicity and speed.

Surface-enhanced Raman spectroscopy (SERS) biosensor

SERS is a technique that strongly amplifies the Raman scattering effect of analyzed molecules. These molecules are located in the "hot spots" of metal nanostructures and are characterized by strong local field enhancement caused by SPR [111]. SERS technology is also known for its high sensitivity and specificity, which can be used to detect, identify and characterize various compounds or biomaterials. As a result, specific and unique fingerprints of the compounds/materials studied can be obtained. SERS technology has a wide range of practical application prospects because of its high sensitivity, high selectivity and the possibility of rapid, unmarked and non-destructive analysis [112]. Since the 1990s, the field of SERS analysis of microorganisms has attracted special attention of researchers [113]. Tycova and others have reviewed the microfluidic devices based on SERS for the detection of bacteria in drinking water [114]. Cheng et al. [33] designed a new microfluidic platform, which uses hybrid electric matrix to enrich bacteria to the stagnation area on a rough electrode with SERS activity in the center of the concentric circular electrode. The results showed that the isolation and enrichment of bacteria were completed in 3 min, and the enhancement factor increased by about 1000 times in the local area of about 5000 μm2 with a low bacterial concentration of 5 × 103 CFU/mL. Escherichia coli, S. aureus and P. aeruginosa can be successfully identified in 1 min without using antibodies/chemical reagents during fixation and reaction. The detection limits are 1 × 104, 3 × 103, 5 × 103 CFU/mL, respectively. Pu et al. [115] investigated the latest application of SERS microfluidic platforms in the detection of food contaminants, including pesticides, antibiotic residues and foodborne pathogens. At present, this technology has been widely used in pathogen detection, including various food substrates. Since the preparation of SERS substrate generally requires gold or silver nanoparticles as the enhanced substrate, the selection of target molecules is greatly limited in the current study of microfluidic SERS sensors with immobilized metal nanostructures [116]. Li et al. [117] designed a microfluidic SERS sensor for rapid, label-free detection of biomolecules. This is the first report of the integration of nanoporous gold core-related materials into the microfluidic chip to increase the total surface area of available nanostructures, thus providing more adsorption sites for biomolecules. Nanoporous gold disk (NPGD) array, an efficient SERS substrate, was monolithically integrated into a microfluidic chip. The device employs Rhodamine 6G to quantify the spatial uniformity and determine the shortest detection time to verify the performance of the sensor. Next, the sensor can detect dopamine and urea biomolecules. The sensor has unprecedented detection limits and speed compared to other microfluidic SERS sensors. In the past few decades, centrifugal microfluidic technology has made great progress, showing the rapid and automatic implementation of complex analysis. Krafft et al. [118] developed a feasible, low-cost chip laboratory device, equipped with nanoporous films to connect two stacked vertical microfluidic channels. One of the channels provides the sample, while the second channel attracts the sample through the potential energy driving force. Silver nanoparticles were added to the sample by the SERS effect. The pores of the membrane act as filters to trap microbiomes and nanoparticle clusters, creating suitable conditions for sensitive SERS detection. In this study, the optimized experimental parameters were used to analyze the common pathogenic bacteria (E. coli DH5VLB120 and Pseudomonas Huangshan VLB120) in tap water after adding water. The system can construct a one-time optical detection platform to realize the coupling of electrokinetic concentration of pathogens on integrated nanoporous membranes and SERS detection (Fig. 4C). The device offers fast and simple operation, shows small dimensions, is durable and can be used as a disposable device. Therefore, it has high potential in rapid POC analysis of bacterial samples, especially used in combination with portable Raman instruments. Keys et al. [119] developed a new, rugged, low-cost cavity array SERS substrate fabrication technology and integrated it into microfluidic devices. The manufacturing depends on the two-photon 3D printing of the main template and can mass-produce simple modifications of the reproducible array and cavity structure with submicron resolution. By introducing a nanostructure-plasma “hot spot” and directly integrating into microfluidic devices, this method achieves Raman signal enhancement up to 6.7 × 107. The proposed manufacturing method for microfluidic stents and SERS platforms is highly repetitive, and the microstructure of 3D printing or a PDMS master sheet observed after dozens of applications will not deteriorate. Therefore, the reported method allows many times the number of replicas to be made from a single parent microstructure, so it is very economical. In addition, the process is relatively simple and does not require skilled personnel or expensive manufacturing equipment. Therefore, it is hoped that this kind of equipment can be used as a reference in bacterial detection, and more stable enhanced substrates and portable devices can be developed as soon as possible to cope with the challenges brought by the application of POCT, and can gradually improve the ability to integrate concentrated bacteria [118, 120].

Microfluidic chip with electrochemical biosensor detection methods

An impedance biosensor is a typical electrochemical biosensor, which has the advantages of compact design, fast detection speed, relatively low cost, and easy integration, so it has become an ideal choice for biosensor applications. In general, impedance biosensors require simpler devices, are easily integrated with electronic reading devices, and are less vulnerable to environmental impacts and pollution than other analytical technologies [121]. Some impedance biosensor-based microfluidic technology that has been developed for the detection of foodborne pathogenic bacteria can provide high specific surface area, small volume, strong sample treatment ability, short detection time, automatic operation, and no cross contamination. Dastider et al. [35] proposed a low-cost, easy-to-manufacture biosensor that can detect S. typhimurium quickly and accurately. This study also compares the advantages of microfluidic biosensors with non-microfluidic biosensors. The biosensor can provide qualitative and quantitative results within 3 h without any enrichment steps. The detection limit of the microfluidic biosensor is 3 × 103 CFU/mL compared to the 3 × 104 CFU/mL for the non-microfluidic biosensor, which indicates that the sensitivity of the microfluidic biosensor is increased tenfold (Fig. 5A). In addition, the samples used for bacteria detection in microfluidic biosensors are very small, usually at the microliter or nanoliter level. Even if biosensors can detect bacteria as low as one cell, that is, the detection limit is 103 CFU/mL or 106 CFU/mL, they are still not sensitive enough to detect foodborne pathogens because most bacteria should not be found in food samples. Yao et al. [17] developed a microfluidic impedance biosensor for rapid, sensitive continuous-flow detection of E. coli O157:H7. After coupling of MNPs modified by streptavidin and biotin-tagged polyclonal antibodies (PABS) to form immune MNPs, the target bacteria were first isolated from the background using MNPs to form an MNP-bacterial complex. Then, the AuNPs modified by urease and an adaptor against E. coli O157:H7 were conjugated with MNP-bacteria to form a MNP-bacteria-AuNPs-urease complex. Finally, the complex was used to catalyze the hydrolysis of urea to produce ammonium carbonate, resulting in reduced impedance. The impedance was measured online with the biosensor, and the concentration of E. coli O157:H7 was determined by impedance normalization analysis. There was a good linear relationship between the relative change rate of impedance and the concentration of bacteria and the detection limit was 12 CFU/mL (Fig. 5B). A MNP-Listeria-AuNPs-urease sandwich complex was prepared by adding AuNPs. The capture rate of Listeria cells could reach 93%, and the capture time could be shortened to 30 min. The detection limit of Listeria cells was as low as 1.6 × 102 CFU/mL. The detection time was shortened from 2 h to 1 h, and the standard recovery rate of lettuce samples was 82.1–89.6% [14]. The detection method captures the complex in the separation chip by applying a high-gradient magnetic field and uses active magnetic mixing, which leads to a rapid immune reaction and greatly improves the capture efficiency of L. monocytogenes in the separation chip. Within 30 min, the capture efficiency reached ~ 93%. Research shows that a microfluidic impedance biosensor has the potential to isolate and detect bacteria online, automatically and sensitively. To reduce the steps of bacterial screening and proliferation, Jiang et al. [122] proposed a method based on pathogen concentration and Mn2+ impedance analysis to reduce SiO2@MnO2 nanocomposites for rapid quantification of S. typhimurium in food samples. In this biosensor, SiO2@MnO2 nanocomposites were reduced to Mn2+ by H2O2, magnetic bead-modified monoclonal antibody for capturing S. typhimurium was used for IMS, and the enrichment process was about 45 min. The biosensor has a detection limit of 21 CFU/mL for S. typhimurium in added milk samples (Fig. 5C). This method can effectively avoid the interference of food matrix in the detection of foodborne S. typhimurium. The crossed array microelectrode can be used to determine the change of ionic strength and can be reused more than 100 times. Joint detection technology can be further integrated into the immunosensor part of the chip laboratory to track foodborne pathogens in real time, and can be extended to the detection and quantification of other biological macromolecules and harmful compounds. In the future, the development of a variety of hybrid systems coupled with a microfluidic device, as well as the application of intelligent detection equipment and electrochemical integration technology, such as smartphones and microelectronic devices, will provide the basic guarantees for the rapid development of microfluidic technology.

Fig. 5
figure 5

(A) 3D schematic of the impedance biosensor showing the electrode array embedded under a microchannel with (a) inlet and (b) outlet. (c) Cross-sectional profile demonstrating various layers of the impedance biosensor. (d) Fabricated and packaged device. Reproduced from [35] with permission of Hindawi. (B) The principle of the microfluidic impedance biosensor based on immunomagnetic separation and urease catalysis for continuous-flow detection of E. coli O157:H7. Reproduced from [17] with permission of Elsevier. (C) Schematic diagram of impedance biosensor for detection of S. typhimurium with a cross-finger microelectrode array based on immunomagnetic separation. Reproduced from [122] with permission of Elsevier

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Conclusions and prospects

Because of their inherent advantages such as high integration, good accuracy, low reagent consumption, and fast reaction speed, microfluidic chips are becoming a hot research topic in food testing. There are benefits from the rapid development of micro-processing technology, which is expected to provide integrated complex detection and diagnosis functions. In the future, the main development directions of microfluidics are as follows:

  1. 1.

    Generally speaking, it is difficult for biosensors to detect low levels of bacteria, partly because of the size of the sample, the long incubation time for bacteria, the many washing and drying steps required to process the sample, and the insufficient sensitivity caused by the interference of the food matrix. These are the challenges in develo** sensor systems. The automatic flow control delivery on the microfluidic chip is a necessary condition for the realization of the POCT application of pathogen bacteria detection, which can minimize sample processing of unskilled operators. Therefore, the combination of a microfluidic chip and more powerful smartphone application software for field analysis, while providing fast transmission and data storage for tracking and recording, is a future research direction.

  2. 2.

    Although microfluidic technology shows high potential in the detection of pathogenic bacteria, it is still greatly limited when used commercially. When a microfluidic sensor is designed in an environment with limited resources, other factors must be considered. In particular, the efficient capture of multiple pathogenic bacteria from complex samples for high-throughput multiplex analysis is still a key problem to be solved. This is mainly due to the lack of a "bio-recognition element" with high binding affinity, although some bio-recognition elements used for specific binding of pathogenic bacteria are described in detail in this paper. However, most biosensors still use antibodies, so it is necessary to further develop microfluidic chip technology to improve the specific recognition ability of pathogenic bacteria. In order to realize the portability, ease of use, multiplex reusability and sensitivity of pathogenic bacteria detection, enhance the adaptability of the instrument to the environment and improve the reliability of the instrument is the key to the commercialization of microfluidics.

  3. 3.

    Isolating the target bacteria from the sample background is an important part of bacteria detection. MNPs feature large specific surface area, small steric hindrance, and uniform distribution. They are usually modified with antibodies against the target bacteria, react with the bacteria, and then magnetically isolate them with a strong magnetic field to remove the background, concentrating bacteria in a volume of buffer. So far, because the IMS usually uses manual operations, it is not suitable for separation from large-volume (10 mL or more) target bacterial samples to improve sensitivity. Although high-gradient magnetic separation (HGMS) has been investigated as a method for the separation of target bacteria in large-volume samples, the blocking of separation channels by large particles in actual samples and the nonspecific binding of proteins and other residues have greatly restricted its use in complex food samples. It is very important to develop effective methods for the isolation and enrichment of pathogenic bacteria.

  4. 4.

    Metal organic skeleton nanoparticles (MOFNP) can be used not only as an electrode interface, but also as an oxidative beacon, which combines microfluidics and biosensor technology to detect foodborne pathogens to a great extent. The existence of sources of pathogenic bacteria can also aid in establishing an early diagnostic system for foodborne diseases to reduce the incidence and associated mortality.

  5. 5.

    The μPAD has become a promising tool and can provide a variety of analytical applications for the analysis of pathogenic bacteria in POCT. Compared with traditional microfluidic devices, the μPAD has significant advantages: it is economical, easy to manufacture, disposable, and portable, so researchers can integrate methods to control fluid transport in paper channels, such as layer assembly, the use of wax valves, or the patterning of hydrophobic and hydrophilic substrates in the channel. When fluid transmission is programmed, complex detection schemes can be completed automatically without the need for an external control system. Fluid flow can be better managed programmatically, and multi-target analytes can be detected with high sensitivity by using one-step or multistep analysis schemes. The μPAD is most suitable for onsite health screening with high-throughput function. This plays an important role in early diagnosis (if isolation is necessary) and treatment to eliminate the spread of infectious or deadly diseases throughout the community, especially in the event of an epidemic or pandemic.

In summary, there are many promising applications in the field of rapid and automated detection methods for foodborne pathogens. In view of the wide applicability and great potential of these methods, there exist great development opportunities in the near future. At present, smart microfluidics technology can realize autonomous, precise operation and control of trace liquids, and has been applied to cell screening, drug delivery, real-time detection, and organ chips.