Abstract
The present study evaluated the influence of the marine bacteria Bacillus cereus Mc-1 on the corrosion of 1020 carbon steel, 316L stainless steel, and copper alloy. The Mc-1 strain was grown in a modified ammoniacal citrate culture medium (CFA.ico-), CFA.ico- with sodium nitrate supplementation (NO3-), and CFA.ico- with sodium chloride supplementation (NaCl). The mass loss and corrosion rate were evaluated after the periods of 7, 15, and 30 days. The results showed that in CFA.ico- and CFA.ico- medium added NO3- the corrosion rates of carbon steel and copper alloy were high when compared to the control. Whereas the medium was supplemented with NaCl, despite the rates being above the averages of the control system, they were considerably below the previous results. In general, the corrosion rates induced by Mc-1 on 316L coupons were below the results compared to carbon steel and copper alloy. When analyzing the corrosion rate measurements, regardless of the culture medium, the corrosion levels decreased consistently after 15 days, being below the levels evaluated after 7 days of the experiment. Our analyses suggest that B. cereus Mc-1 has different influences on corrosion in different metals and environmental conditions, such as the presence of NO3- and NaCl. These results can help to better understand the influence of this bacteria genus on the corrosion of metals in marine environments.
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs00203-021-02607-w/MediaObjects/203_2021_2607_Fig1_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs00203-021-02607-w/MediaObjects/203_2021_2607_Fig2_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs00203-021-02607-w/MediaObjects/203_2021_2607_Fig3_HTML.png)
Similar content being viewed by others
References
Abdoli L, Suo X, Li H (2016) Distinctive colonization of Bacillus sp. bacteria and the influence of the bacterial biofilm on electrochemical behaviors of aluminum coatings. Colloids Surf B Biointerfaces 145:688–694. https://doi.org/10.1016/j.colsurfb.2016.05.075
Aïmeur N, Houali K, Hamadou L, Benbrahim N, Kadri A (2015) Influence of strain Bacillus cereus bacterium on corrosion behaviour of carbon steel in natural sea water. Corr Eng Sci Tech 50:579–588. https://doi.org/10.1179/1743278215Y.0000000022
Angell P, Machowski WJ, Paul PP, Wall CM, Lyle FF Jr (1997) A multiple chemostat system for consortia studies on microbially influenced corrosion. J Microbiol Methods 30:173–178. https://doi.org/10.1016/S0167-701297.00057-2
ASTM G1-03 (2017) Standard practice for preparing, cleaning, and evaluating corrosion test specimens. ASTM International, West Conshohocken, PA
Bonifay V, Wawrik B, Sunner J, Snodgrass EC, Aydin E, Duncan KE, Callaghan AV, Oldham A, Liengen T, Beech I (2017) Metabolomic and metagenomic analysis of two crude oil production pipelines experiencing differential rates of corrosion. Front Microbiol 8:99. https://doi.org/10.3389/fmicb.2017.00099
Boudaud N, Coton M, Coton E, Pineau S, Travert J, Amiel C (2010) Biodiversity analysis by polyphasic study of marine bacteria associated with biocorrosion phenomena. J Appl Microbiol 109:166–179
Capão A, Moreira-Filho P, Garcia M, Bitati S, Procópio L (2020) Marine bacterial community analysis on 316L stainless steel coupons by Illumina MiSeq sequencing. Biotechnol Lett 42:1431–1448. https://doi.org/10.1007/s10529-020-02927-9
Conrad R, Klose M, Noll M (2009) Functional and structural response of the methanogenic microbial community in rice field soil to temperature change. Environ Microbiol 11(7):1844–1853. https://doi.org/10.1111/j.1462-2920.2009.01909.x
Dang H, Lovell CR (2015) Microbial surface colonization and biofilm development in marine environments. Microbiol Mol Biol Rev 80(1):91–138. https://doi.org/10.1128/MMBR.00037-15
Dawood Z, Brozel VS (1998) Corrosion-enhancing potential of Shewanella putrefaciens isolated from industrial cooling waters. J Appl Microbiol 84:929–936
Dong ZH, Liu T, Liu HF (2011) Influence of EPS isolated from thermophilic sulphate-reducing bacteria on carbon steel corrosion. Biofouling 27:487–495. https://doi.org/10.1080/08927014.2011.584369
Enning D, Garrelfs J (2014) Corrosion of iron by sulfate-reducing bacteria: new views of an old problem. Appl Environ Microbiol 80:1226–1236
Gadd GM (2004) Microbial influence on metal mobility and application for bioremediation. Geoderma 122:109–119. https://doi.org/10.1016/j.geoderma.2004.01.002
Garcia M, Procópio L (2020) Distinct profiles in microbial diversity on carbon steel and different welds in simulated marine microcosm. Curr Microbiol 77:967–978. https://doi.org/10.1007/s00284-020-01898-4
Guo Z, Liu T, Cheng YF, Guo N, Yin Y (2017) Adhesion of Bacillus subtilis and Pseudoalteromonas lipolytica to steel in a seawater environment and their effects on corrosion. Colloids Surf B Biointerfaces 157:157–165. https://doi.org/10.1016/j.colsurfb.2017.05.045
Hamilton WA (2003) Microbially influenced corrosion as a model system for the study of metal microbe interactions: a unifying electron transfer hypothesis. Biofouling 19:65–76. https://doi.org/10.1080/0892701021000041078
Hu Y, **ao K, Zhang D, Yi P, **ong R, Dong C, Wu J, Li X (2019) Corrosion acceleration of printed circuit boards with an immersion silver layer exposed to Bacillus cereus in an aerobic medium. Front Microbiol 10:1493. https://doi.org/10.3389/fmicb.2019.01493
Jayaraman A, Ornek D, Duarte DA, Lee CC, Mansfeld FB, Wood TK (1999) Axenic aerobic biofilms inhibit corrosion of copper and aluminum. Appl Microbiol Biotechnol 52:787–790. https://doi.org/10.1007/s002530051592
Kato S (2016) Microbial extracellular electron transfer and its relevance to iron corrosion. Microb Biotechnol 9:141–148. https://doi.org/10.1111/1751-7915.12340
Kato S, Yumoto I, Kamagata Y (2015) Isolation of acetogenic bacteria that induce biocorrosion by utilizing metallic iron as the sole electron donor. Appl Environ Microbiol 81:67–73. https://doi.org/10.1128/AEM.02767-14
Koch GH, Varney J, Thompson NO, Moghissi O, Gould M, Payer JH (2016) NACE International IMPACT report 2016.
Kokilaramani S, AlSalhi MS, Devanesan S, Narenkumar J, Rajasekar A, Govarthanan M (2020) Bacillus megaterium-induced biocorrosion on mild steel and the effect of Artemisia pallens methanolic extract as a natural corrosion inhibitor. Arch Microbiol 202(8):2311–2321. https://doi.org/10.1007/s00203-020-01951-7
Lee AK, Newman DK (2003) Microbial iron respiration: impacts on corrosion processes. Appl Microbiol Biotechnol 62:134–139. https://doi.org/10.1007/s00253-003-1314-7
Li X-X, Liu J-F, Zhou L, Mbadinga SM, Yang S-Z, Gu J-D, Mu B-Z (2017) Diversity and composition of sulfate-reducing microbial communities based on genomic DNA and RNA transcription in production water of high temperature and corrosive oil reservoir. Front Microbiol 8:1011. https://doi.org/10.3389/fmicb.2017.01011
Li S, Li L, Qu Q, Kang Y, Zhu B, Yu D, Huang R (2019) Extracellular electron transfer of Bacillus cereus biofilm and its effect on the corrosion behaviour of 316L stainless steel. Colloids Surf B Biointerfaces 173:139–147. https://doi.org/10.1016/j.colsurfb.2018.09.059
Little B, Lee J, Ray R (2007) A review of “green” strategies to prevent or mitigate microbiologically influenced corrosion. Biofouling 23:87–97. https://doi.org/10.1080/08927010601151782
Maia M, Capão A, Procópio L (2019) Biosurfactant produced by oil-degrading Pseudomonas putida AM-b1 strain with potential for microbial enhanced oil recovery. Bioremed J 23:302–310. https://doi.org/10.1080/10889868.2019.1669527
Marconnet C, Dagbert C, Roy M, Féron D (2008) Stainless steel ennoblement in freshwater: from exposure tests to mechanism. Corr Sci 50:2342–2352. https://doi.org/10.1016/j.corsci.2008.05.007
Marty F, Gueuné H, Malard E, Sánchez-Amaya JM, Sjögren L, Abbas B, Quillet L, van Loosdrecht MC, Muyzer G (2014) Identification of key factors in accelerated low water corrosion through experimental simulation of tidal conditions: influence of stimulated indigenous microbiota. Biofouling 30:281–297. https://doi.org/10.1080/08927014.2013.864758
McBeth JM, Emerson D (2016) In situ microbial community succession on mild steel in estuarine and marine environments: exploring the role of iron-oxidizing bacteria. Front Microbiol 7:767. https://doi.org/10.3389/fmicb.2016.00767
McBeth JM, Little BJ, Ray RI, Farrar KM, Emerson D (2011) Neutrophilic iron-oxidizing “Zetaproteobacteria” and mild steel corrosion in nearshore marine environments. Appl Environ Microbiol 77:1405–1412. https://doi.org/10.1128/AEM.02095-10
Moradi M, Sun Z, Song Z, Hu H (2019) Effect of proteases secreted from a marine isolated bacterium Bacillus vietnamensis on the corrosion behaviour of different alloys. Bioelectrochemistry 126:64–71. https://doi.org/10.1016/j.bioelechem.2018.08.003
Moura V, Ribeiro I, Moriggi P, Capão A, Salles C, Bitati S, Procópio L (2018) The influence of surface microbial diversity and succession on microbiologically influenced corrosion of steel in a simulated marine environment. Arch Microbiol 200:1447–1456. https://doi.org/10.1007/s00203-018-1559-2
Mumford AC, Adaktylou IJ, Emerson D (2016) Peeking under the iron curtain: development of a microcosm for imaging the colonization of steel surfaces by Mariprofundus sp. strain DIS-1, an oxygen-tolerant fe-oxidizing bacterium. Appl Environ Microbiol 82(22):6799–6807
Muyzer G, Stams AJ (2008) The ecology and biotechnology of sulphate-reducing bacteria. Nat Rev Microbiol 6:441–454. https://doi.org/10.1038/nrmicro1892
NACE RP-07-75 (2005) Standard recommended practice, preparation, installation, analysis and interpretation of corrosion coupons in oilfield operations. NACE International, Houston
Procópio L (2020a) Changes in microbial community in the presence of oil and chemical dispersant and their effects on the corrosion of API 5L steel coupons in a marine-simulated microcosm. Appl Microbiol Biotechnol 104:6397–6411. https://doi.org/10.1007/s00253-020-10688-8
Procópio L (2020b) The era of ‘omics’ technologies in the study of microbiologically influenced corrosion. Biotechnol Lett 42:341–356. https://doi.org/10.1007/s10529-019-02789-w
Procópio L (2020c) Microbial community profiles grown on 1020 carbon steel surfaces in seawater-isolated microcosm. Ann Microbiol 70:13. https://doi.org/10.1186/s13213-020-01547-y
Rajasekar A, Anandkumar B, Maruthamuthu S, Ting YP, Rahman PK (2010) Characterization of corrosive bacterial consortia isolated from petroleum-product-transporting pipelines. Appl Microbiol Biotechnol 85:1175–1188. https://doi.org/10.1007/s00253-009-2289-9
Ribeiro I, Moura V, Moriggi P, Pereira S, Procopio L (2017) Steel corrosion by iron oxidant bacteria isolated from sea water. Inter J Biosci 11:240–246. https://doi.org/10.12692/ijb/11.3.240-246
Selvaraj C, Sivakamavalli J, Vaseeharan B, Singh P, Singh SK (2014) Examine the characterization of biofilm formation and inhibition by targeting SrtA mechanism in Bacillus subtilis: a combined experimental and theoretical study. J Mol Model 20:2364. https://doi.org/10.1007/s00894-014-2364-8
Sheng X, Ting YP, Pehkonen SO (2008) The influence of ionic strength nutrients and pH on bacterial adhesion to metals. J Colloid Interface Sci 321(2):256–264. https://doi.org/10.1016/j.jcis.2008.02.038
Suma MS, Basheer R, Sreelekshmy BR, Riyas AH, Bhagya TC, Ameen Sha M, Shibli SMA (2019) Synergistic action of Bacillus subtilis, Escherichia coli and Shewanella putrefaciens along with Pseudomonas putida on inhibiting mild steel against oxygen corrosion. Appl Microbiol Biotechnol 103(14):5891–5905. https://doi.org/10.1007/s00253-019-09866-0
Wan H, Song D, Zhang D, Du C, Xu D, Liu Z, Ding D, Li X (2018) Corrosion effect of Bacillus cereus on X80 pipeline steel in a Bei**g soil environment. Bioelectrochemistry 121:18–26. https://doi.org/10.1016/j.bioelechem.2017.12.011
Wang YS, Liu L, Fu Q, Sun S, An ZY, Ding R, Li Y, Zhao XD (2020) Effect of Bacillus subtilis on corrosion behavior of 10 MnNiCrCu steel in marine environment. Sci Rep 10:5744
Watanabe K, Manefield M, Lee M, Kouzuma A (2009) Electron shuttle in biotechnology. Curr Opin Biotechnol 20:633–641. https://doi.org/10.1016/j.copbio.2009.09.006
Xu D, Li Y, Song F, Gu T (2013) Laboratory investigation of microbiologically influenced corrosion of C1018 carbon steel by nitrate reducing bacterium Bacillus licheniformis. Corr Sci 77:385–390. https://doi.org/10.1016/j.corsci.2013.07.044
Xu D, Li Y, Gu T (2016) Mechanistic modeling of biocorrosion caused by biofilms of sulfate reducing bacteria and acid producing bacteria. Bioelectrochemistry 110:52–58. https://doi.org/10.1016/j.bioelechem.2016.03.003
Yang G-C, Zhou L, Mbadinga SM, Liu J-F, Yang S-Z, Gu J-D, Mu B-Z (2016) Formate-dependent microbial conversion of CO2 and the dominant pathways of methanogenesis in production water of high-temperature oil reservoirs amended with bicarbonate. Front Microbiol 7:365. https://doi.org/10.3389/fmicb.2016.00365
Yuan SJ, Pehkonen SO (2007) Microbiologically influenced corrosion of 304 stainless steel by aerobic Pseudomonas NCIMB 2021 bacteria: AFM and XPS study. Colloids Surf B Biointerfaces 59:87–99
Zhang Y, Ma Y, Duan J, Li X, Wang J, Hou B (2019) Analysis of marine microbial communities colonizing various metallic materials and rust layers. Biofouling 35(4):429–442. https://doi.org/10.1080/08927014.2019.1610881
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by Erko Stackebrandt.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Moreira-Filho, P., de Paula da Silva Figueiredo, P., Capão, A. et al. The influence of the marine Bacillus cereus over carbon steel, stainless corrosion, and copper coupons. Arch Microbiol 204, 9 (2022). https://doi.org/10.1007/s00203-021-02607-w
Received:
Revised:
Accepted:
Published:
DOI: https://doi.org/10.1007/s00203-021-02607-w