Abstract
Mesoscale heterogeneity of alpine landscapes generates snowmelt gradients resulting in a distinct vegetation zonation from almost snow-free fellfields to long-lasting snowbeds. Although the vegetative trait variation along such gradients has been intensively studied, little is known about whether and how seed germination is adapted to the variable snowpack duration. Here, we compare the seed germination niches of 18 characteristic plant species occurring in four distinct plant communities (alpine lichen heath—Festuca varia grassland—Geranium-Hedysarum meadow—snowbed) along an alpine snowmelt gradient in the North Caucasus (Russia). In a fully factorial experiment, we tested seed germination responses to temperature (10/2, 14/6, 18/10, 22/14, 26/18 and 30/22 °C) and water potential (0, − 0.2, − 0.4, − 0.6 and − 0.8 MPa) gradients, reflecting the full spectrum of seedling establishment conditions in the study system. Community-specific germination niches were identified by generalised mixed model with Bayesian estimation. Our study revealed that the species from the four focal communities significantly differed in their temperature requirements for germination, whereas soil moisture was found to be a limiting factor for seedling establishment along the entire gradient. The snowbed germination was constrained to comparatively high temperatures above 10 °C, whereas seed germination in alpine lichen heaths occurred under a wide range of experimental conditions. The seed germination patterns of species from Festuca varia grasslands and Geranium-Hedysarum meadows reflected their intermediate position along the snowmelt gradient. We conclude that seed germination niche is affected by the environmental filtering along the snowmelt gradient thus departing from the general alpine germination syndrome.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Alpine habitats occur worldwide above the natural treeline (Körner and Paulsen 2004; Körner et al. 2011; Testolin et al. 2020) and are characterized by low temperatures, unstable substrates and short growing seasons (Körner 2021). Their dependence on extreme and strict climatic conditions (Körner and Paulsen 2004), coupled with the relatively small terrestrial area they occupy (Testolin et al. 2020), could make alpine environments disproportionally vulnerable to climate change (Pickering et al. 2014; Huss et al. 2017). Climate change has caused some upwards shifts of alpine plant species (Rumpf et al. 2019) and these shifts could ultimately lead to extinction if plant species were to find themselves in a position where they have nowhere higher to retreat. Nonetheless, the complex alpine topography creates a mosaic of microhabitats varying greatly in microclimatic conditions (Scherrer and Körner 2009). This high topographical variation could help alpine vegetation to cope with climate change by providing local micro-refugia (Körner and Hiltbrunner 2021).
Mesoscale habitat heterogeneity in combination with wind and solar radiation generates snowmelt gradients in the alpine belt, driving the distinct vegetation zonation from almost snow-free windswept crests to long-lasting snowbeds (Onipchenko 1994; Stanton et al. 1994; Choler 2005). Snow depth and snowmelt patterns strongly influence surface-level temperature, moisture and light, three key abiotic factors determining plant growth and reproduction in the corresponding communities (Winkler et al. 2018; Körner 2021). The sparse, thin snowpack on steep, leeward slopes (fellfields) melts early resulting in freezing soil in winter, frost damage in spring and drought stress during summer. Contrastingly, thick, long-lasting snowpack in flat, wind-protected snowbeds protects plants from freezing temperatures in winter and spring and supplies them water during the early summer. At the same time, delayed snowmelt in the snowbeds restricts photosynthetic carbon gain, depletes carbohydrate reserves, stimulates growth of pathogenic fungi or encourages winter herbivory by small mammals (Stanton et al. 1994; Kudo et al. 1999; Shimono and Kudo 2005).
Although alpine plants usually are capable of clonal reproduction (Körner 2021), sexual reproduction via diaspores (i.e. seeds, fruits, or fruits with extra-ovarian structures attached; hereafter ‘seeds’) is essential to maintain genetic diversity and to migrate to suitable new sites in response to climatic changes (Fernández-Pascual et al. 2019). However, the short alpine growing seasons limit the timespan for flowering, pollination, seed development and seed dispersal. For successful regeneration, seeds must germinate in the most appropriate period to ensure seedling survival (Bell and Bliss 1980; Forbis 2003). The physiological process of seed germination is therefore an essential life stage that must be timed to occur when the environment is favourable for subsequent seedling survival and growth (Poschlod et al. 2013). This has led to a widespread alpine seed germination strategy characterized primarily by physiological seed dormancy, a strong need for cold stratification to break dormancy and warm-cued germination (Fernández-Pascual et al. 2021; Carta et al. 2022). However, this common strategy most likely hides important variability among species. For example, alpine species show higher optimal temperature for germination than either subalpine species (i.e. species that live close to the treeline; Fernández-Pascual et al. 2017) or their congeneric counterparts from below the treeline (Walder and Erschbamer 2015). Differences in germination traits have been attributed also to species’ successional niche and habitat preferences, with pioneer species germinating better at colder temperatures than later successional species (Schwienbacher et al. 2012), while species from calcareous and siliceous alpine grasslands respectively show a slow overwinter germination or high germination under all conditions (Tudela-Isanta et al. 2018a, b). Surprisingly, not enough attention has been paid to germination strategies along the snowmelt and growing-season-length gradient determined by mesotopographical variations, despite their high importance for alpine vegetation responses to climate change (Körner and Hiltbrunner 2021). To our knowledge, this question has only been tackled directly by Shimono and Kudo (2005), who found no habitat-related germination differences when comparing fellfield and snowbed habitats in Japan. The remaining published evidence on seed germination behaviour along the snowmelt gradient is limited to experiments [e.g. Bell and Bliss (1980)] or field observations of a very few snowbed species; [Shimono and Kudo (2001)].
The importance of micro- and mesotopographic variation for alpine plant ecology makes it necessary to study it in more detail, in other mountain regions, and with updated methodologies. In this study, we compare the seed germination niches of characteristic plant species of four distinct plant communities (alpine lichen heath—Festuca varia grasslands—Geranium-Hedysarum meadows—snowbeds) occurring along a snowmelt gradient in the alpine belt of the North Caucasus. Using experimental data on 18 species, we tested the hypothesis that species responses to germination temperature and water potential will depend on their optimal position in the topographic gradient. Specifically, we expected the snowbed germination to be confined to comparatively high temperatures and high water supply, conditions typical for subnivean soils shortly after snowmelt in this habitat (Björk and Molau 2007). In alpine lichen heaths, communities occurring at the snow-poor end of the gradient, we expected seeds to be able to germinate at comparatively low soil water contents, due to the low water supply from the melting snowpack and the fast drying skeletal soils (Onipchenko 2004). Likewise, we expected them to have broader temperature requirements for germination, thus displaying an opportunistic germination phenology whenever soils become relatively moist. The seed germination pattern in Festuca varia grasslands and Geranium-Hedysarum meadows, two communities occupying the middle parts of the toposequence, were expected to display intermediate seed germination responses to temperature and water with tendencies towards their snow-poor and snow-rich neighbors, respectively.
Materials and methods
Study area
This study was conducted in the Teberda National Park (Karachaevo-Cherkessian Republic, the Northwestern Caucasus, Russia), in the alpine belt of the Malaya Khatipara mountain (N43.4398, E41.6749). The local climate is typically temperate-zone montane with mean annual temperature of − 1.2 °C and annual precipitation of 1400 mm. The warmest month is August with a mean monthly temperature of + 8.3 °C, but frost can occur throughout summer (Onipchenko 2004). Siliceous parent rocks predominate in the study area and soils are Umbric Leptosols (Malysheva et al. 2004).
Snow accumulation is the main factor determining the composition and structure of alpine plant communities in the Reserve (Onipchenko 1994). Depth and duration of snow cover is in turn controlled by landscape position (slope orientation and tilt). Northwestern Caucasian alpine plant communities can be arranged along a zonation of snowmelt gradient as shown in Fig. 1 and described in the next section (see also Table 1 and Fig. 2).
Study plant communities
Alpine lichen heaths (ALH: Fig. 2A) occupy windward crests and steep slopes with no or little snow accumulation (up to 30 cm snow depth) that results in a comparatively long growing season of about 5 months. The low-productive ALH communities develop on shallow, skeletal soils with comparatively low microbial activity, low water holding capacity and low nutrient content (Vertelina et al. 1996). Deep freezing (down to 200 cm) is typical for the ALH soils. The ALHs are mainly dominated by fruticose lichens (e.g. Cetraria islandica, Cladonia gracilis, Flavocetraria cucullata and Thamnolia vermicularis), dwarf shrub Vaccinium vitis-idea and to a smaller extent by graminoids (e.g. Festuca ovina, Carex sempervirens, C. umbrosa) and herbs (Anemone speciosa, Antennaria dioica, Campanula tridentata).
Festuca varia grasslands (FVG; Fig. 2B) occur on dry, moderate to steep, mainly southern slopes with little snow accumulation (about 0.5–1 m), and the growing season lasts about 4 months. Soils under these communities are thick, well developed and with comparatively low soil skeleton content (10% by volume in the upper 10 cm; (Grishina et al. 1993). Dense tussock narrow-leaved grasses Festuca varia and Nardus stricta strongly dominate these communities. Due to high litter accumulation in the community (Leinsoo et al. 1991), the co-occurring xeromorphic herbs (e.g. Ajuga orientalis, Campanula collina, Galium verum, Sempervivum caucasicum, Viola altaica) are confined to more or less open gaps between the dense cover of grasses.
Geranium-Hedysarum meadows (GHM; Fig. 2C) occupy lower parts of leeward, gentle slopes and shallow depressions with maximal snow depth in winter of 2–3 m and the growing seasons of 2.5–3.5 months. The soils in the GHM communities are thick, have comparatively high water holding capacity and contain the largest amount of soil nutrients among the four study communities. Soil properties in combination with the favourable snow regime explain the fact the GHMs are the most productive communities among the studied vegetation types in the alpine belt of the National Park (Onipchenko 2004). GHMs are mainly dominated by two herbs, Geranium gymnocaulon and Hedysarum caucasicum, with a few other herbs (e.g. Carum meifolium, Pedicularis condensata and Pulsatilla aurea) being the constant constituents in these meadows. Some bunch grasses, such as Festuca brunnescens, F. varia and Nardus stricta, are frequent associates, though they are usually not abundant in the community (Onipchenko 2004). One peculiar characteristic of the GHM communities is the high activity of pine voles (Microtus majori) that maintain soil water and air permeability, as they intensify soil biological processes by burrowing (Onipchenko 2004).
Snow bed communities (SBC; Fig. 2D) occur in deep depressions and lower, flat parts of leeward northern and eastern slopes with heavy winter snow accumulation (5 m and even more). Snow melting ends in late July or the first half of August, thus SBCs have the shortest vegetation season among the alpine communities (1.5–2.5 months). As compared to other study communities, soils under the SBCs are rich in nitrogen, have high water content and the lowest skeleton content. Nonetheless, the short growing period leads to low productivity of these potentially productive communities. The SBCs are composed of low-stature (2–3 cm), short-rosette or cree** plants (e.g. Sibbaldia procumbens, Minuartia aizoides and Taraxacum stevenii), forming a dense herbaceous cover without pronounced layers.
Soil temperature measurements
Soil temperatures were measured in each of the study communities from September 2008 to September 2009 with the help of temperature loggers (Thermochron iButtons DS1921G#F5; Maxim Integrated Products, Inc., Sunnyvale, CA, United States) every 4 h. To reduce the effects of frost heaving on the temperature measurements, loggers were buried at the depth of 10 cm. Our preliminary data analysis (results are not shown) indicates that the soil temperatures at this depth correlate well with the soil surface temperatures. We used the soil temperature logs to calculate the number of days of snow cover in each plant community, estimated as days with no diurnal temperature variations between the night and the day (Zhang 2005). We used these values as a predictor variable in the seed germination model (see below).
Seed germination experiment
Fully ripened seeds and fruits (thereafter ‘seeds’) of 18 species typical for each of the focal communities were collected in the end of growing season in 2017 (Online Appendix 1). The focal species were selected based on their community fidelity (i.e. clear ecological preferences for one of the study communities) and standing biomass contribution to the communities (i.e. species with comparatively high abundance). A large dataset of vegetation surveys conducted in the region was used as a basis for the species selection (Onipchenko V., unpublished data).
Seeds of each species were collected in the community the species represents. The seeds were collected from at least 20 randomly selected individuals growing at least 2 m apart from each other. After collection, seeds were air-dried for several days, cleaned manually, and allowed to after-ripen in a paper bag at room conditions for 3 months followed by dry storage in a cold room at + 4 °C until the beginning of the germination experiments.
To reveal the requirements for optimal germination as an adaptation to environmental conditions in the study communities, we used a five-level full factorial experiment to test seed germination responses to temperature and water, two major environmental drivers of the physiological process of seed germination (Bewley et al. 2013). To compare the temperature and water threshold for germination across species, we conducted the experiments with non-dormant seeds. Seeds of Arenaria lychnoides and Nardus stricta were cold-wet stratified at + 4 °C for 3 months to break physiological dormancy. Physical seed dormancy in Anthyllus vulneraria and Trifolium polyphyllum was broken by kee** the seeds in boiling water for 10 s (Rosbakh et al. 2020a). Seeds of the remaining species did not require any dormancy-breaking pre-treatment as they readily germinated with high percentages (> 80%) in a preliminary experiment.
In each experimental treatment and species, three replicates of 20 seeds were placed in a Petri dish on two layers of filter paper moistened with 5 ml of either distilled water or a polyethylene glycol 6000 solution (PEG; see below). Due to low seed filling rates the number of seeds in Poaceae was increased to 30. Seeds were incubated in RUMED 1301 climatic chambers (Rubarth Apparate GmbH, Laatzen, Germany) at diurnal fluctuating temperatures with 14 h of light supplied by white fluorescent tubes and 10 h of darkness. Germination (cotyledon appearance) was monitored weekly for 6 weeks; germinated seeds were removed during the checks. After the experiments were terminated, the viability of non-germinated seeds was checked by inspection of embryos. Seeds with white and firm embryos were considered viable (Baskin and Baskin 2014).
Seeds were germinated along two gradients of temperature and water potential representing the germination conditions that seeds may encounter along the snowmelt gradient. To test temperature requirements for germination, we selected six temperatures (10/2, 14/6, 18/10, 22/14, 26/18 and 30/22 °C; 14/10 h day/night photoperiod). The effect of water potential on seed germination was studied by incubating the seeds at five water potentials (0, − 0.2, − 0.4, − 0.6 and − 0.8 MPa) of PEG solutions prepared according to Michel and Kaufmann (1973). Petri dishes were sealed with Parafilm to maintain relatively constant water potential during the entire experiment; filter paper remained moist for the entire duration of the experiment.
Data analysis
To test the hypothesis that community-specific responses of seed germination to temperature and water varied along the snowmelt gradient, we fitted a generalised mixed model with Bayesian estimation (Markov Chain Monte Carlo generalised linear Mixed model, MCMCglmm) using the package ‘MCMCglmm’ in R (Hadfield 2010). MCMCglmm allows to model seed germination as a binomial response variable, take into account the lack of independence caused by the shared phylogenetic history of the study species, and consider more than one sample (i.e. Petri dish) per species.
The response variable in the model was the final seed germination proportion. The fixed predictors were as follows: (1) snow cover length (i.e. number of days of snow cover estimated with the soil temperature logs), (2) germination temperature, (3) water potential, plus (4) the interactions snow length × germination temperature and (5) snow length × water potential. We did not include the interaction temperature × water potential nor the three-way interaction because they fall outside the questions of the experiment, and further we checked that they were non-significant in a separate model.
To account for non-independence in the data caused by the existence of different study species which are linked by a shared phylogeny, the model included as random factors species identity and a reconstructed phylogenetic tree of the study species. To create the phylogeny, we used the R package V.PhyloMaker (** and Qian 2019) that contains an updated mega-tree of the seed plants based on Smith and Brown (2018). We placed taxa absent from the mega-tree at the genus-level basal node.
We used weakly informative priors for the model, with parameter-expanded priors for the random effects. Each model was run for 500,000 MCMC steps, with an initial burn-in phase of 50,000 and a thinning interval of 50 (Villemereuil and Nakagawa 2014) resulting, on average, in 9000 posterior distributions. From the resulting posterior distributions, we calculated mean parameter estimates and 95% highest posterior density and credible intervals (CI). We estimated the significance of model parameters by examining CIs, considering parameters with CIs overlap** with zero as non-significant. The R script to fit the model is included as a supplementary material (Online Appendix 2).
Results
Soil temperatures in the study communities
Soil temperatures (Fig. 3) measured from September 2008 to September 2009 varied along the snowmelt gradient. The soil of the alpine lichen heath community showed thermal variation below zero between January and April, indicating that snow cover was little or non-existent, and therefore the soil was not insulated from the air. Conversely, the other three communities showed a flat temperature of zero during winter, suggesting that they were under the cover of snow and insulated from external air variation. Time of snowmelt also varied along the gradient as follows: from mid-May in the alpine lichen heath, to early June in the Festuca varia grassland, late June in the Geranium-Hedysarum meadowand early July in the snowbed community.
Seed germination patterns in the study species
Study species differed considerably in their seed germination response to the experimental gradients of temperature and water varying from narrow to broad germination niche (Online Appendix 3). As for the former, seeds of Festuca varia, Hedysarum caucasicum, Phleum alpinum and Trifolium polyphyllum germinate over a broad range of temperatures and water potentials, except for the − 0.8 MPa. As for the latter, seed germination in Anthoxantum odoratum, Potentilla verna and Taraxacum stevenii was limited to comparatively high temperatures (> 15 °C) and moderate water potentials (from − 0.4 to − 0.6 MPa). The germination patterns of the remaining species varied between these two extremes.
Community-specific seed germination patterns
The MCMCglmm (Fig. 4) indicated that snow length per se did not have a significant main effect on germination: the average germination proportion across germination treatments was not different between the four communities. Germination temperature and water potential had significant and positive main effects: overall, germination was higher at higher temperatures and at comparatively higher water potentials. Germination temperature and snow depth had a significant positive interaction: species from communities with higher snow depth (e.g. the snowbeds) had their germination more limited to the warmer end of germination temperatures (Fig. 5). The interaction between water potential and snow depth was not significant, indicating that the different communities did not differ in their response to water potential (Fig. 5). The community-specific germination patterns at the most optimal for germination conditions (water potential of 0 MPa (pure water) or incubation temperature of 22/14 °C), are shown in Fig. 6.
Discussion
Alpine seed germination has been suggested to follow a common strategy aimed at increasing seedling establishment chances under harsh environmental conditions (Fernández-Pascual et al. 2021; Carta et al. 2022). The general tendency to a warm-cued germination detected for the study species suggests that North Caucasian alpine plants employ the same strategy to postpone seed germination to warmer summer months characterised by a comparatively low probability of severe frost events negatively affecting seedling of alpine plants (Rosbakh and Poschlod 2015); Rosbakh et al. 2020b). In its turn, the overall positive effects of low osmotic potentials on the germination of the focal species confirms the evidence that seedling establishment in terrestrial habitats with seasonal climates, including arctic and alpine environments (Bell and Bliss 1980; Oberbauer and Miller 1982; Tudela-Isanta et al. 2018b), is water-limited (Orsenigo et al. 2015; Walder and Erschbamer 2015). From the ecological point of view, it implies that alpine seed germination is triggered by comparatively high soil moisture levels (e.g. after a snowmelt or summer rainfalls), a key adaptation to avoid seedling emergence on commonly desiccated surfaces of summer alpine soils (Körner 2021) known to be one of the main reasons for seedling mortality in alpine regions (Welling and Laine 2000; Forbis 2003). High soil moisture is necessary for germination and seedling establishment because small seeds do not contain sufficient carbohydrate reserves for rapid production of deep roots in a drying environment (Oberbauer and Miller 1982).
Analysing the community-specific germination patterns, we revealed a clear separation of the germination niches along the snowmelt gradient regarding temperature only (Figs. 4 and 5). As expected, the main germination in snowbed species was constrained to comparatively high temperatures above 10 °C (Figs. 5 and 6) corresponding well with the in situ soil temperature conditions after the snowmelt (Fig. 3). This temperature range corroborates seed germination experiments in the lab and field observations on Italian (Bernareggi et al. 2016) and Japanese snowbed species (Shimono and Kudo 2001, 2005) suggesting a broader occurrence of snowbed germination syndrome in alpine ecosystems. Such high temperature requirements for snowbed germination seem to be adaptive in preventing germination under the snow cover, in years with smaller snow accumulation, or unexpectedly earlier onset of snowmelt. At the same time, emergence of seedlings in the middle of the growing season exposes them to favorable environmental conditions as soil and air temperatures are close to their maxima, meltwater protects from soil desiccation and daylight period is long [reviewed in Björk and Molau (2007)]. In other words, the late start of the growing season is compensated by favorable growth and development conditions that allow juvenile plants to quickly attain a critical size and develop adaptations required to winter survival (Shimono and Kudo 2001, 2005).
In contrast to our hypothesis, snowbed species did not differ in their germination requirements for water from the species occurring on the other three communities and were able to germinate under moderate levels of water availability (ca. − 0.5 MPa; Figs. 5 and 6). This finding suggests that snowbed species might not depend entirely on habitat-specific ample meltwater supply during germination and can tolerate short-term summer droughts common in years with low precipitation.
In accordance with our hypothesis, seed germination in alpine lichen heaths (ALH) was found to occur under a wide range of experimental conditions including lower temperatures (Fig. 5), thus departing from the general alpine germination syndrome (Fernández-Pascual et al. 2021). Such opportunistic strategy for non-dormant seeds to germinate equally under conditions of frost damage in spring (Fig. 2) and drought stress in summer (Onipchenko 2004) can be explained by biotic interactions of the seedlings and surrounding vegetation. First, lichens have been shown to facilitate seedling recruitment in alpine heaths by buffering extreme soil temperatures and soil drying under droughts (Nystuen et al. 2019). Second, the vascular plants occurring in these communities could also protect germinated seeds from, e.g. frost damage (Rosbakh et al. 2021), thereby additionally enhancing seedling establishment (Margreiter et al. 2021). As a result, seeds of alpine lichen heath plants may germinate with equal success throughout the growing the season. Finally, the broad seed germination niche could be explained by the high relative energy investment into offspring by the ALH species and their short-term persistence in soil seed banks (Onipchenko et al. 1998): once produced, seeds should germinate or die.
Finally, our experimental results confirmed our expectation that the intermediate position of the Festuca varia grassland and Geranium-Hedysarum meadow communities along the snowmelt gradient was reflected in their community-specific germination patterns. Although the species from these two communities did not differ in their requirements for water, they tended to have similar temperature requirements of their neighboring communities. The tendency for higher germination temperatures in the communities with intermediate snow length could be attributed to the vegetation structure in the Festuca varia grasslands and Geranimum-Hedysarum meadows. Most likely, seedling establishment in such productive communities with tall and dense vegetation is possible only in gaps caused by animal activities (Onipchenko et al. 1998) with reduced levels of competition (Fenner et al. 2005). Early germination in such gaps is linked with high probability of frost injury of maladapted alpine seedlings (Rosbakh et al. 2020b), particularly during the night radiation frost events (Körner 2021). The onset of comparatively high temperatures in later summer months with reduced frost probability triggers then the seed germination in the corresponding communities.
Conclusions and implications
Our study reveals that, in addition to vegetative traits (Choler 2005; Pickering et al. 2014; Körner 2021), seed germination niche is also affected by environmental filtering along the snowmelt gradient. We clearly demonstrate that species from four focal communities differencing in snowmelt patterns significantly differ in their temperature requirements for germination, whereas soil moisture was found to be a limiting factor for seedling establishment along the entire gradient. These findings provide further support to the recent argument that vegetation ecology research should consider traits related to germination, as they represent different ecological niche axes (Jiménez-Alfaro et al. 2016; Rosbakh et al. 2021).
The revealed community-specific germination responses to temperature have important implications not only for alpine ecological research in general, but also for climate change research and conservation/restoration ecology. The recent climate warming in the North Caucasus has already affected the timing of snowmelt leading to detectable/considerable changes in the alpine vegetation, with those at the extreme end of the snowmelt gradient (snowbeds and Geranium-Hedysarum meadow) experiencing the most fundamental change (Elumeeva et al. 2013). With even greater reductions in winter precipitation and continuous rising temperatures predicted for the study region, the altered snowpack thickness and duration will inevitably affect regeneration from seeds in the study communities. Particularly, earlier snowmelt in snowbeds may relax this environmental filter allowing species with less demanding requirements for germination from the other communities to colonize and establish in this unique habitat (Hülber et al. 2011; Elumeeva et al. 2013; Pickering et al. 2014).
References
Baskin CC, Baskin JM (2014) Seeds: ecology, biogeography, and evolution of dormancy and germination. Elsevier, Academic Press, San Diego
Bell KL, Bliss LC (1980) Plant reproduction in a high Arctic environment. Arct Alp Res 12:1–10. https://doi.org/10.1080/00040851.1980.12004158
Bernareggi G, Carbognani M, Mondoni A, Petraglia A (2016) Seed dormancy and germination changes of snowbed species under climate warming: the role of pre- and post-dispersal temperatures. Ann Bot 118:529–539. https://doi.org/10.1093/aob/mcw125
Bewley JD, Bradford KJ, Hilhorst HWM, Nonogaki H (2013) Germination. In: Bewley B et al (eds) Seeds: physiology of development. Springer, pp 133–181
Björk RG, Molau U (2007) Ecology of alpine snowbeds and the impact of global change. Arct Antarct Alp Res 39:34–43. https://doi.org/10.1657/1523-0430(2007)39[34:EOASAT]2.0.CO;2
Carta A, Fernández-Pascual E, Gioria M, Müller JV, Rivière S, Rosbakh S, Saatkamp A, Vandelook F, Mattana E (2022) Climate shapes the seed germination niche of temperate flowering plants: a meta-analysis of European seed conservation data. Ann Bot. https://doi.org/10.1093/aob/mcac037
Choler P (2005) Consistent shifts in alpine plant traits along a mesotopographical gradient. Arct Antarct Alp Res 37:444–453. https://doi.org/10.1657/1523-0430(2005)037[0444:CSIAPT]2.0.CO;2
Elumeeva TG, Onipchenko VG, Egorov AV, Khubiev AB, Tekeev DK, Soudzilovskaia NA, Cornelissen JHC (2013) Long-term vegetation dynamic in the Northwestern Caucasus: which communities are more affected by upward shifts of plant species? Alp Bot 123:77–85. https://doi.org/10.1007/s00035-013-0122-7
Fenner MK, Fenner M, Thompson K (2005) The ecology of seeds. Cambridge University Press, Cambridge
Fernández-Pascual E, Jiménez-Alfaro B, Bueno Á (2017) Comparative seed germination traits in alpine and subalpine grasslands: higher elevations are associated with warmer germination temperatures. Plant Biol J 19:32–40. https://doi.org/10.1111/plb.12472
Fernández-Pascual E, Mattana E, Pritchard HW (2019) Seeds of future past: climate change and the thermal memory of plant reproductive traits. Biol Rev 94:439–456. https://doi.org/10.1111/brv.12461
Fernández-Pascual E, Carta A, Mondoni A, Cavieres LA, Rosbakh S, Venn S, Satyanti A, Guja L, Briceño VF, Vandelook F, Mattana E, Saatkamp A, Bu H, Sommerville K, Poschlod P, Liu K, Nicotra A, Jiménez-Alfaro B (2021) The seed germination spectrum of alpine plants: a global meta-analysis. New Phytol 229:3573–3586. https://doi.org/10.1111/nph.17086
Forbis TA (2003) Seedling demography in an alpine ecosystem. Am J Bot 90:1197–1206. https://doi.org/10.3732/ajb.90.8.1197
Grishina LA, Onipchenko VG, Makarov MI, Vanyasin VA (1993) Changes in properties of mountain-meadow alpine soils of the northwestern Caucasus under different ecological conditions. Eurasian Soil Sci 25:1–12
Hadfield JD (2010) MCMC methods for multi-response generalized linear mixed models: the MCMCglmmR package. J Stat Softw 33:1–22. https://doi.org/10.18637/jss.v033.i02
Hülber K, Bardy K, Dullinger S (2011) Effects of snowmelt timing and competition on the performance of alpine snowbed plants. Perspect Plant Ecol Evol Syst 13:15–26. https://doi.org/10.1016/j.ppees.2011.01.001
Huss M, Bookhagen B, Huggel C, Jacobsen D, Bradley RS, Clague JJ, Vuille M, Buytaert W, Cayan DR, Greenwood G, Mark BG, Milner AM, Weingartner R, Winder M (2017) Toward mountains without permanent snow and ice. Earth’s Future 5:418–435. https://doi.org/10.1002/2016EF000514
Jiménez-Alfaro B, Silveira FA, Fidelis A, Poschlod P, Commander LE (2016) Seed germination traits can contribute better to plant community ecology. J Veg Sci 27:637–645. https://doi.org/10.1111/jvs.12375
** Y, Qian H (2019) V.PhyloMaker: an R package that can generate very large phylogenies for vascular plants. Ecography 42:1353–1359. https://doi.org/10.1111/ecog.04434
Körner C (2021) Alpine plant life: functional plant ecology of high mountain ecosystems. Springer Nature
Körner C, Hiltbrunner E (2021) Why Is the alpine flora comparatively robust against climatic warming? Diversity 13:383. https://doi.org/10.3390/d13080383
Körner C, Paulsen J (2004) A world-wide study of high altitude treeline temperatures. J Biogeogr 31:713–732. https://doi.org/10.1111/j.1365-2699.2003.01043.x
Körner C, Paulsen J, Spehn EM (2011) A definition of mountains and their bioclimatic belts for global comparisons of biodiversity data. Alp Bot 121:73. https://doi.org/10.1007/s00035-011-0094-4
Kudo G, Nordenhäll U, Molau U (1999) Effects of snowmelt timing on leaf traits, leaf production, and shoot growth of alpine plants: Comparisons along a snowmelt gradient in northern Sweden. Écoscience 6:439–450. https://doi.org/10.1080/11956860.1999.11682543
Leinsoo TN, Onipchenko VG, Leinsoo TA, Velikanov LL (1991) Biomass variation of soil micromycetes in the alpine communities in the Northwest Caucasus. Mikol Fitopatol 25:206–212
Malysheva TI, Makarov MI, Onipchenko VG, Volkov AV, Bulatnikova IV (2004) Soils and nutrient turnover. In: Onipchenko VG (ed) Alpine ecosystems in the Northwest Caucasus. Springer Netherlands, Dordrecht, pp 9–23
Margreiter V, Walde J, Erschbamer B (2021) Competition-free gaps are essential for the germination and recruitment of alpine species along an elevation gradient in the European Alps. Alp Bot 131:135–150. https://doi.org/10.1007/s00035-021-00264-9
Michel BE, Kaufmann MR (1973) The osmotic potential of polyethylene glycol 6000. Plant Physiol 51:914–916. https://doi.org/10.1104/pp.51.5.914
Nystuen KO, Sundsdal K, Opedal ØH, Holien H, Strimbeck GR, Graae BJ (2019) Lichens facilitate seedling recruitment in alpine heath. J Veg Sci 30:868–880. https://doi.org/10.1111/jvs.12773
Oberbauer S, Miller PC (1982) Effect of water potential on seed germination. Ecography 5:218–220. https://doi.org/10.1111/j.1600-0587.1982.tb01040.x
Onipchenko VG (1994) The structure and dynamics of alpine plant communities in the Teberda Reserve, the Northwestern Caucasus. Oecol Mont 3:40–50
Onipchenko VG (2004) Alpine ecosystems in the Northwest Caucasus. Springer Science & Business Media
Onipchenko VG, Semenova GV, van der Maarel E (1998) Population strategies in severe environments: alpine plants in the northwestern Caucasus. J Veg Sci 9:27–40. https://doi.org/10.2307/3237220
Orsenigo S, Abeli T, Rossi G, Bonasoni P, Pasquaretta C, Gandini M, Mondoni A (2015) Effects of autumn and spring heat waves on seed germination of high mountain plants. PLoS ONE 10:e0133626. https://doi.org/10.1371/journal.pone.0133626
Pickering C, Green K, Barros AA, Venn S (2014) A resurvey of late-lying snowpatches reveals changes in both species and functional composition across snowmelt zones. Alp Bot 124:93–103. https://doi.org/10.1007/s00035-014-0140-0
Poschlod P, Abedi M, Bartelheimer M, Drobnik J, Rosbakh S, Saatkamp A (eds) (2013) Seed ecology and assembly rules in plant communities. Wiley Online Books
Rosbakh S, Poschlod P (2015) Initial temperature of seed germination as related to species occurrence along a temperature gradient. Funct Ecol 29:5–14. https://doi.org/10.1111/1365-2435.12304
Rosbakh S, Baskin CC, Baskin JM (2020a) Nikolaeva et al.’s reference book on seed dormancy and germination. Ecology 101:e03049. https://doi.org/10.1002/ecy.3049
Rosbakh S, Margreiter V, Jelcic B (2020b) Seedlings of alpine species do not have better frost-tolerance than their lowland counterparts. Alp Bot 130:179–185. https://doi.org/10.1007/s00035-020-00237-4
Rosbakh S, Chalmandrier L, Phartyal S, Poschlod P (2021) Inferring community assembly processes from functional seed trait variation along temperature gradient. bioRxiv. https://doi.org/10.1101/2021.12.17.473108
Rumpf SB, Hülber K, Zimmermann NE, Dullinger S (2019) Elevational rear edges shifted at least as much as leading edges over the last century. Glob Ecol Biogeogr 28:533–543. https://doi.org/10.1111/geb.12865
Scherrer D, Körner C (2009) Infra-red thermometry of alpine landscapes challenges climatic warming projections. Glob Change Biol 16:1. https://doi.org/10.1111/j.1365-2486.2009.02122.x
Schwienbacher E, Navarro-Cano JA, Neuner G, Erschbamer B (2012) Correspondence of seed traits with niche position in glacier foreland succession. Plant Ecol 213:371–382
Shimono Y, Kudo G (2001) Pattern of seedling emergence of alpine plants: comparisons between fellfield and snowbed habitats (scientific note). Mem Natl Inst Polar Res 54:467–478
Shimono Y, Kudo G (2005) Comparisons of germination traits of alpine plants between fellfield and snowbed habitats. Ecol Res 20:189–197. https://doi.org/10.1007/s11284-004-0031-8
Smith SA, Brown JW (2018) Constructing a broadly inclusive seed plant phylogeny. Am J Bot 105:302–314. https://doi.org/10.1002/ajb2.1019
Stanton ML, Rejmánek M, Galen C (1994) Changes in vegetation and soil fertility along a predictable snowmelt gradient in the Mosquito Range, Colorado, U. S. A. Arct Alp Res 26:364–374. https://doi.org/10.1080/00040851.1994.12003081
Testolin R, Attorre F, Jiménez-Alfaro B (2020) Global distribution and bioclimatic characterization of alpine biomes. Ecography 43:779–788. https://doi.org/10.1111/ecog.05012
Tudela-Isanta M, Fernández-Pascual E, Wijayasinghe M, Orsenigo S, Rossi G, Pritchard HW, Mondoni A (2018a) Habitat-related seed germination traits in alpine habitats. Ecol Evol 8:150–161. https://doi.org/10.1002/ece3.3539
Tudela-Isanta M, Ladouceur E, Wijayasinghe M, Pritchard HW, Mondoni A (2018b) The seed germination niche limits the distribution of some plant species in calcareous or siliceous alpine bedrocks. Alp Bot 128:83–95. https://doi.org/10.1007/s00035-018-0199-0
Vertelina OV, Onipchenko VG, Makarov MI (1996) Primary minerals and processes of weathering in high-mountain soils of the Teberda Reserve. Vestnik Moskovskogo Universiteta, seria 17. Pochvovedenie 51:1–8
Villemereuil de P, Nakagawa S (2014) General quantitative genetic methods for comparative biology. In: Garamszegi LZ (ed) Modern phylogenetic comparative methods and their application in evolutionary biology. Springer, pp 287–303
Walder T, Erschbamer B (2015) Temperature and drought drive differences in germination responses between congeneric species along altitudinal gradients. Plant Ecol 216:1297–1309. https://doi.org/10.1007/s11258-015-0509-1
Welling P, Laine K (2000) Characteristics of the seedling flora in alpine vegetation, subarctic Finland, I. Seedling densities in 15 plant communities. Ann Bot Fenn 37:69–76
Winkler DE, Butz RJ, Germino MJ, Reinhardt K, Kueppers LM (2018) Snowmelt timing regulates community composition, phenology, and physiological performance of alpine plants. Front Plant Sci 9:1140. https://doi.org/10.3389/fpls.2018.01140
Zhang T (2005) Influence of the seasonal snow cover on the ground thermal regime: an overview. Rev Geophys 43:R4002. https://doi.org/10.1029/2004RG000157
Acknowledgements
We thank Peter Poschlod and University of Regensburg (Germany) for providing lab infrastructure for the experimental part of the study. We further thank Maxi Bleicher, Julia Eberl, Bernardica Jelcic, Elena Kazantseva Martina Kiermaier, Kay Knackfuss und Irina Weinberger for the help with seed germination experiments. S.R. was partially supported by BayHost scholarship programme. E.F.P. was supported by the Jardín Botánico Atlántico [SV-20-GIJON-JBA]. VO thanks Russian Science Foundation (project #19-14-00038p) for financial support.
Funding
Open Access funding enabled and organized by Projekt DEAL.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare no competing interests.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Rosbakh, S., Fernández-Pascual, E., Mondoni, A. et al. Alpine plant communities differ in their seed germination requirements along a snowmelt gradient in the Caucasus. Alp Botany 132, 223–232 (2022). https://doi.org/10.1007/s00035-022-00286-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00035-022-00286-x