Abstract
The suitability was evaluated of MNNG as a mutagen inducing increased frequencies of mutations in the cell populations of three strains of chlorococcal algae for the purposes of selection. MNNG has proved to be highly toxic to those algae as it produces severe physiological responses of the affected cells. The mutagenic effect of MNNG was relatively small in comparison with the recorded toxic effect. From these results it has been concluded that in reverse to NEU, MNNG can hardly be applied with such good an effect in the mutation breeding of chlorococcal algae that are suitable for mass cultivation.
Abstract
Hodnotila se vhodnost MNNG pro indukování zvýšených frekvencí mutací v buněčných populacích tří kmenů chlorokokálních řas, aby v nich bylo možno provádět výběry podle různých zvolených znaků a vlastností. Ukázalo se, že MNNG je pro tyto řasy velmi toxický a že u nich vyvolává některé nepříznivé fysiologické reakce, které se intensivně projevují u ovlivněných buněk. Mutagenní účinek MNNG se jeví u chlorokokálních řas poměrně nízký při srovnání se zjištěným toxickým účinkem. Z výsledků uvedených v této práci vyplývá, že MNNG není za daných podmínek zdaleka tak vhodným mutagenem pro chlorokokální řasy, jakým se ukázala v předcházejících pracích N-ethyl-N-nitrosomočovina. Použití MNNG v mutačním šlechtění chlorokokálních řas vhodných pro hromadné kultivace bez dalších opatření, která by odstranila jeho toxicitu, není zdaleka tak vhodné jako u N-ethyl-N-nitrosomočoviny.
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Nečas, J. Physiological and mutagenic effects of N-methyl-N′-nitro-N-nitrosoguanidine in populations of chlorococcal algae. Biol Plant 17, 130–138 (1975). https://doi.org/10.1007/BF02920974
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DOI: https://doi.org/10.1007/BF02920974