Summary
The effectiveness of ethyl methanesulfonate (EMS) as a mutagen inChlamydomonas reinhardi has been demonstrated in two different systems: reversion to prototrophy of an argine-requiring strain and forward mutation to auxotrophy of the wild-type strain. In these experiments, the toxic effect of EMS was very low. With certain batches of EMS, an unusually high toxicity was observed. In this case, the lethal effects seem to be attributable to the presence of minor impurities in EMS.
Similar content being viewed by others
References
Froese-Gertzen, E. E., C. F.Konzak, R. J.Foster, and R. A.Nilan: Correlation between some chemical and biological reactions of ethyl methanesulfonate. Nature (Lond.)198, 447 (1963).
Lindegren, G., H. L.Hwang, Y.Oshima, and C. C.Lindegren: Genetical mutants induced by ethyl methanesulfonate inSaccharomyces. Canad. J. Genet. Cytol.7, 491–499 (1965).
Loppes, R.: Nature du milieu sélectif et expression phénotypique des mutations induites par le méthane sulfonate d'éthyl (EMS) chezChlamydomonas reinhardi. Ann. Inst. Pasteur110, 645–654 (1966a).
—: Damage induced by methyl methanesulfonate (MMS) inChlamydomonas reinhardi. Z. Vererbungsl.98, 193–202 (1966b).
Loveless, A., and S.Howarth: Mutation of bacteria at high levels of survival by ethyl methanesulphonate. Nature (Lond.)184, 1780–1782 (1959).
Rannenberg, H., u. C. G.Arnold: Die Wirkung von Äthylmethansulfonat aufChlamydomonas reinhardi. Molec. Gen. Genetics101, 212–216 (1968).
Author information
Authors and Affiliations
Additional information
Communicated byC. Auerbach
Chargé de Recherches au Fonds National de la Recherche Scientifique.
Rights and permissions
About this article
Cite this article
Loppes, R. Ethyl methanesulfonate: an effective mutagen inChlamydomonas reinhardi . Molec. Gen. Genet. 102, 229–231 (1968). https://doi.org/10.1007/BF00385978
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00385978