Abstract
Using a degenerated primer and a T-primer, a MADS-box gene,M79, was amplified by RT-PCR from rice fluorescence at meiosis stage and then cloned. Sequence analysis shows thatM79 shares 98.2% homology withOsMADS7 at DNA level while only 92% at the amino acid level. The transcript ofM79 possesses five different polyadenylation sites. Only a single copy ofM79 gene has been found in rice genome, which is located on chromosome 8.M79 is expressed specifically in flower organs, from pre-meiosis stage through pollen maturation. Ectopic expression ofM79 in T0 and T1 transgenic rice results in early-flowering, implying thatM79 is involved in controlling the flowering time. In the same time,M79 may be involved in controlling the branching process to make more flower buds.
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The EMBL accession number for the nucleotide sequence ofM79 is Y15008.
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Qu, L., Zhang, Y., Li, C. et al. Expression pattern and functional analysis of a MADS-box geneM79 from rice. Sci. China Ser. C.-Life Sci. 44, 161–169 (2001). https://doi.org/10.1007/BF02879321
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DOI: https://doi.org/10.1007/BF02879321