Abstract
Characteristics of mouse macrophage (MP) cell lines A640-BB-2, J774.1 and P388D1 and mouse peritoneal exudate MPs were studied and compared in cell morphology, ability to recognize tumor cells in the presence and absence of OK-432 known to activate MPs, and in lysosomal enzyme activity. In A640-BB-2 cells and exudate MPs, cell surfaces showed a few ridge-like processes and microvilli; spontaneous cytotoxicity was moderate against tumor target L929, and little or absent against targets SV3T3, B-16 and U937; and lysosomal enzyme activity of nonspecific esterase, acid phosphatase, and β-glucuronidase was high. After culture in the presence of OK-432, A640-BB-2 cells and exudate MPs showed more extensive spreading with larger surface areas and with increased numbers of ridge-like processes and microvilli, and their cytotoxicity against target L929 became more extensive. The stable soluble factor did not participate in the mechanism of cytotoxicity against target L929 mediated by A640-BB-2 cells and exudate MPs. J774.1 and P388D1 cells were different from exudate MPs in cell morphology and ability to recognize tumor cells when cultured either with or without OK-432, and in lysosomal enzyme activity. A640-BB-2 cells seem to be useful in studying MP-tumor cell interaction and MP activation, and in detecting the trace biological activating factor of MPs.
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Abbreviations
- DEM:
-
Dulbecco's modified Eagle's medium
- MP:
-
macrophage
- PBS:
-
phosphate-buffered saline
- SEM:
-
scanning electron microscopy
References
Barca, T and Anderson, PJ (1962) Histochemical method for acid phosphatase using hexazonium pararosalimin as coupler. J. Histochem. Cytochem. 10: 741–754.
Chapes, SK, Didier, ES and Tompkins, WAF (1988) Macrophage cell line B6MP102 resembles peritoneal macrophages in tumor cell recognition and killing. J. Leukocyte Biol. 43: 28–35.
Chen, TR (1977) In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp. Cell Res. 104: 255–262.
Cohen, S (1977) The role of cell-mediated immunity in the induction of inflammatory responses. Am. J. Pathol. 88: 503–528.
Fukumoto, S, Tanigawa, T, Yazaki, S and Tanaka, Y (1991) Cytotoxicity of mouse macrophage cell line, A640-BB-2, against newborn larvae of Trichinella spiralis. Yonago Acta Medica 34: 105–112.
Hayashi, M, Nakajima, Y and Fishman, W (1964) The cytologic demonstration of β-glucuronidase employing naphthol AS-BI glucuronidase and hexazonium pararosalimin. A preliminary report. J. Histochem. Cytochem. 12: 239–247.
Inoue, T and Osatake, N (1988) A new drying method of biological specimens for scanning electron microscopy: the t-butyl alcohol freeze-drying method. Arch. Histol. Cytol. 51: 53–59.
Johnson, CR, Kitz, D and Little, JR (1983) A method for the derivation and continuous propagation of cloned murine bone marrow macrophages. J. Immunol. Meth. 65: 319–332.
Koren, HS, Handwerger, BS and Wunderlich, JR (1975) Identification of macrophage-like characteristics in a cultured murine tumor line. J. Immunol. 114: 894–897.
Leder, LD (1964) Über die selektive fermentcytochemishe Darstellung von neutrophilen myeloischen Zellen und Gewebsmastzellen im Paraffinschnitt. Klin. Wochenschr. 42: 553.
Onozaki, K, Homma, Y, Miura, K and Hashimoto, T (1981) Scanning electron microscopic study of changes in the surfaces of lymphokine-treated guinea pig macrophages. J. Reticuloendothel. Soc. 30: 471–481.
Polliack, A and Gordon, S (1975) Scanning electron microscopy of murine macrophages. Surface characteristics during maturation, activation, and phagocytosis. Lab. Invest. 33: 469–477.
Ralph, P, Prichard, J and Cohn, M (1975) Reticulum cell sarcoma: An effector cell in antibody-dependent cell-mediated immunity. J. Immunol. 114: 898–905.
Ralph, P and Nakoinz, I (1981) Differences in antibody-dependent cellular cytotoxicity and activated killing of tumor cells by macrophage cell lines. Cancer Res. 41: 3546–3550.
Raschke, WC, Baird, S, Ralph, P and Nakoinz, I (1978) Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261–267.
Snyderman, R, Pike, MC, Fischer, DG and Koren, HS (1977) Biologic and biochemical activities of continuous macrophage cell lines P388D1 and J774.1. J. Immunol. 119: 2060–2066.
Takayama, H, Katsumoto, T, Ohno, K, Nakaso, A, Takagi, A and Kimura, G (1983) In situ electron microscopic observation of negatively stained tissue culture cells contaminated with mycoplasmas. J. Gen. Microbiol. 129: 3379–3384.
Tanigawa, T, Takayama, H, Takagi, A and Kimura, G (1983) Cell growth and differentiation in vitro in mouse macrophages transformed by a tsA mutant of simian virus 40 I. Cellular response in proliferative and phagocytic activities to the shift of temperature differs depending on the culture state in mouse bone marrow cells transformed by the tsA640 mutant of simian virus 40. J. Cell. Physiol. 116: 303–310.
Tanigawa, T, Okuda, A, Takayama, H, Yamada, K, Takagi, A and Kimura, G (1984) Cell growth and differentiation in vitro in mouse macrophages transformed by a tsA mutant of simian virus 40 II. Changes in the distribution of DNA content during the reversible transition between macrophage and nonmacrophage state in the cultures of tsA640-transformed macrophages. J. Cell. Physiol. 120: 242–248.
Tanigawa, T, Shimura, H, Yamada, K, Okuda, A, Takayama, H, Takagi, A, Tanaka, Y and Kimura, G (1985) Cell growth and differentiation in vitro in mouse macrophages transformed by a tsA mutant of simian virus 40 III. Large T antigen level and cell proliferation and survival in an SV40 tsA640-transformed macrophage line. J. Cell. Physiol. 125: 19–22.
Treves, AJ, Fuks, Z, Voss, R, Tal, T, Barak, V, Konijn, AM, Kaplan, R and Laskov, R (1984) Establishment of cell lines from somatic cell hybrids between human monocytes and mouse myeloma cells. J. Immunol. 132: 690–694.
van Furth, R, Van Schadewijk-Nieuwstad, M, Elzenga-Claasen, I, Cornelisse, C and Nibbering, P (1985) Morphological, cytochemical, functional and proliferative characteristics of four murine macrophage-like cell lines. Cell. Immunol. 90: 339–357.
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Tanigawa, T., Takayama, H., Osatake, H. et al. Characterization of macrophage cell line A640-BB-2: A640-BB-2 resembles peritoneal exudate macrophages in cell morphology, tumor cell recognition, responsiveness to immunomodulator OK-432 and lysosomal enzyme activity. Cytotechnology 10, 43–51 (1992). https://doi.org/10.1007/BF00376099
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DOI: https://doi.org/10.1007/BF00376099