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Features of the Population of Mouse Peritoneal Macrophages Isolated after Stimulation with Concanavalin A and Thioglycolate

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Murine peritoneal macrophages isolated from the lavage fluid after administration of thioglycolate and concanavalin A are presented by two populations of cells of different diameters. Polarization of macrophages into a proinflammatory (M1) phenotype is accompanied by an increase in number of small cells. Macrophages obtained after administration of thioglycolate demonstrate higher tendency to anti-inflammatory (M2) phenotype, while macrophages isolated after administration of concanavalin A are committed in the proinflammatory direction. Lactate level is increased in M1 macrophages in comparison with M2 cells, which indicates predominance of glycolytic metabolism. Macrophages obtained after administration of concanavalin A have reduced mitochondrial potential, which reflects a tendency to apoptosis. Autophagy activation and inhibition neutralize the differences in pro- and anti-inflammatory properties of polarized macrophages obtained after thioglycolate administration, but have less pronounced effect on macrophages obtained after administration concanavalin A. Autophagy inhibitor increases mitochondrial potential in non-polarized macrophages obtained after administration of concanavalin A. These results demonstrate divergent properties of macrophages obtained after administration of glycolate and concanavalin A due to the difference in the mechanisms of experimental peritonitis.

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Authors and Affiliations

  1. National Research Center for Cardiology, Ministry of Health of the Russian Federation, Moscow, Russia

    E. S. Zubkova, K. V. Dergilev, I. B. Beloglazova, Yu. D. Molokotina, M. A. Boldyreva, Z. I. Tsokolaeva, I. S. Stafeev, M. Yu. Menshikov & Ye. V. Parfyonova

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  1. E. S. Zubkova
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  2. K. V. Dergilev
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  3. I. B. Beloglazova
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  4. Yu. D. Molokotina
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  5. M. A. Boldyreva
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  6. Z. I. Tsokolaeva
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  7. I. S. Stafeev
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  8. M. Yu. Menshikov
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  9. Ye. V. Parfyonova
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Correspondence to M. Yu. Menshikov.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 107-115, June, 2021

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Zubkova, E.S., Dergilev, K.V., Beloglazova, I.B. et al. Features of the Population of Mouse Peritoneal Macrophages Isolated after Stimulation with Concanavalin A and Thioglycolate. Bull Exp Biol Med 171, 532–540 (2021). https://doi.org/10.1007/s10517-021-05265-6

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  • DOI: https://doi.org/10.1007/s10517-021-05265-6

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