Abstract
Rhaponticoides iconiensis (Hub.-Mor.) M.V.Agab. & Greuter is one of the species of Asteraceae family. In this study, the α-amylase and α-glucosidase inhibition activity, the antioxidant, antimicrobial properties and the phytochemical composition of extract and sub-extracts from the leafy stem of R. iconiensis were evaluated. The antimicrobial effect of extracts was tested with the microdilution method and the antioxidant activity was investigated with DPPH·, ABTS·+ and FRAP tests. The phytochemical composition of active sub-extract carried out by LC–MS/MS. As a result, the highest α-glucosidase and α-amylase activity were found in ethyl acetate sub-extract (TSE) of R. iconiensis leafy stem extract (IC50 = 12.108 ± 0.582 µg/mL and 287.733 ± 0.325 µg/mL, respectively). Moreover, the highest total phenolic (312.001 ± 2.008 mgGAE/gextract) and total flavonoid content (242.007 ± 2.646 mgCA/gextract) was found in TSE extract too and this extract was found more active on DPPH· (IC50 = 0.249 ± 0.002 mg/mL), ABTS+· (at a concentration of 0.5 mg/mL equivalent to 0.542 ± 0.002 µM Trolox) and FRAP (at a concentration of 1 mg/mL equivalent to 3642.005 ± 2.646 mmol Fe2+) tests. Besides that, the flavonoids such as apigenin, naringin and patuletin derivatives were determined as the main compounds of active TSE sub-extract. This is the first report that these biological activities investigated and phytochemical compounds were identified in leafy stem extracts of R. iconiensis and it is thought that these studies will be shed light on future studies on this species.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Availability of data and material
All data generated or analyzed during this study are included in this published article.
References
Ali H, Houghton P, Soumyanath A (2006) α-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. J Ethnopharmacol 107:449–455
Alvarado-Sansininea JJ, Sánchez-Sánchez L, López-Muñoz H, Escobar ML, Flores-Guzmán F, Tavera-Hernández R, Jiménez-Estrada M (2018) Quercetagetin and patuletin: antiproliferative, necrotic and apoptotic activity in tumor cell lines. Molecules 23:2579
Chipiti T (2013) In vitro anti-oxidative and carbohydrates digesting enzymes inhibitory effects of some medicinal plants used for the management of diabetes in the Mrewa district, Zimbabwe
Çinbilgel İ, Eren Ö, Duman H (2014) Rhaponticoides gokceoglui (Asteraceae), a striking new species from Turkey. Phytotaxa 170:125–132
Clifford MN, Johnston KL, Knight S, Kuhnert N (2003) Hierarchical scheme for LC-MS n identification of chlorogenic acids. J Agric Food Chem 51:2900–2911
Clinical, Institute LS (2017) Performance standards for antimicrobial susceptibility testing. Clinical and Laboratory Standards Institute, Wayne
Devasahayam G, Burke DJ, Sturgill TW (2007) Golgi manganese transport is required for rapamycin signaling in Saccharomyces cerevisiae. Genetics 177:231–238
Doğu S, Bağcı Y, Dinç M (2009) Rhaponticoidesaytachii sp. nov. (Asteraceae) from south Anatolia Turkey. Nordic J Bot 27:479–482
Eren Ö (2007) The genus Rhaponticoides Vaill. (Asteraceae) in Turkey: a new species and first key. Plant Syst Evol 267:13–23
Greuter W, Raus T (2010) Med-checklist notulae, 28. Willdenowia 39:335–345
Guo C, Yang J, Wei J, Li Y, Xu J, Jiang Y (2003) Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay. Nutr Res 23:1719–1726
Gyamfi MA, Aniya Y (2002) Antioxidant properties of Thonningianin A, isolated from the African medicinal herb, Thonningia Sanguinea. Biochem Pharmacol 63:1725–1737
Köse YB, İşcan G, Göger F, Akalın G, Demirci B, Başer KHC (2016) Chemical composition and biological activity of Centaurea baseri: new species from Turkey. Chem Biodivers 13:1369–1379
Mahmoud AM, Ahmed OM, Ashour MB, Abdel-Moneim A (2015) In vivo and in vitro antidiabetic effects of citrus flavonoids; a study on the mechanism of action. Int J Diabetes Dev Ctries 35:250–263
Martins L (2007) Identity of Serratula bachtiarica Boiss. & Hausskn. and Centaurea bachtiarica Hayek & Bornm. from Iran. Candollea 62:41–43
Negaresh K, Khoshroo SMR, Karamian R, Joharchi MR (2015) A revision of Rhaponticoides (Asteraceae, Cardueae–Centaureinae) from Iran. Phytotaxa 213:087–101
Paşayeva L, Eruygur N, Ayaz F, Tugay O, Fatullayev H (2020a) Assessment of the antioxidant and enzyme inhibition activities of Cousinia iconica with focus on phytochemical investigation by LC-MS/MS. Plant Biosyst 1–11
Paşayeva L, Özalp B, Fatullayev H (2020b) Potential enzyme inhibitory properties of extracts and fractions from fruit latex of Ficus carica-based on inhibition of α-amylase and α-glucosidase. J Food Meas Charact 14:2819–2827
Puntillo D, Peruzzi L (2009) A new species of Rhaponticoides (Asteraceae) from southern Italy. Folia Geobot 44:191
Radan M, Carev I, Tesevic V, Politeo O, Culic VC (2017) Qualitative HPLC-DAD/ESI-TOF-MS analysis, cytotoxic, and apoptotic effects of Croatian endemic Centaurea ragusina L. aqueous extracts. Chem Biodivers 14:e1700099. https://doi.org/10.1002/cbdv.201700099
Ren B et al (2016) Apigenin and naringenin regulate glucose and lipid metabolism, and ameliorate vascular dysfunction in type 2 diabetic rats. Eur J Pharmacol 773:13–23
Roopan SM, Khan N, Rahman F (2009) Synthesis, antioxidant, hemolytic and cytotoxicity activity of AB ring core of mappicine. ARKIVOC Online J Org Chem
Sánchez-Rabaneda F, Jáuregui O, Casals I, Andrés-Lacueva C, Izquierdo-Pulido M, Lamuela-Raventós RM (2003) Liquid chromatographic/electrospray ionization tandem mass spectrometric study of the phenolic composition of cocoa (Theobroma cacao). J Mass Spectrom 38:35–42
Shi L, Ge H-M, Tan S-H, Li H-Q, Song Y-C, Zhu H-L, Tan R-X (2007) Synthesis and antimicrobial activities of Schiff bases derived from 5-chloro-salicylaldehyde. Eur J Med Chem 42:558–564
Tan K, Bancheva S, Vural M, Strid A (2009) Centaurea wagenitziana (Asteraceae: Centaureinae), a new species from the Eastern Balkans. Phytol Balc 15:51–58
Tugay O, Paşayeva L, Demirpolat E, Şahin M (2020) Comparative evaluation of cytotoxicity and phytochemical composition of Centaurea iconiensis (Rhaponticoides iconiensis). Iran J Sci Technol Trans A Sci 45:65–75
Tugay O, Paşayeva L, Demirpolat E, Şahin M (2021) Comparative evaluation of cytotoxicity and phytochemical composition of Centaurea iconiensis (Rhaponticoides iconiensis). Iran J Sci Technol Trans Sci 45:65–75
Wayne P (2008) Clinical and Laboratory Standards Institute: reference method for broth dilution antifungal susceptibility testing of yeasts; approved standard. CLSI document M27-A3 Supplement S 3:6–12
Zheleva-Dimitrova D et al (2020) Identification of bioactive compounds from Rhaponticoides iconiensis extracts and their bioactivities: an endemic plant to Turkey flora. J Pharm Biomed Anal 190:113537
Acknowledgements
The authors thank to Erciyes University Drug Application and Research Center (ERFARMA) for their support in the use of LC-MS/MS in this study.
Funding
This study was supported financially by Erciyes University Scientific Research Projects Coordinating Unit (Project Number THD-2018-7921).
Author information
Authors and Affiliations
Contributions
LP conceptualization, methodology, validation, writing—original draft, writing—review and editing, supervision, resources. Uİ methodology, writing—review and editing. HF conceptualization, methodology, validation, writing—original draft, writing—review and editing. CC validation, writing—original draft, writing—review and editing. OT methodology, validation, writing—original draft, writing—review and editing.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no competing interests.
Ethics approval
Not applicable.
Consent to participate
Not applicable.
Consent for publication
Not applicable.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Paşayeva, L., İnce, U., Fatullayev, H. et al. Hypoglycemic, antioxidant, antimicrobial activities and phytochemical analyses of Rhaponticoides iconiensis leafy stem extracts. Vegetos 34, 592–599 (2021). https://doi.org/10.1007/s42535-021-00250-w
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s42535-021-00250-w