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Isolation and functional characterization of the Brassica napus cruciferin gene cru4 promoter

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Abstract

The 12S globulin protein cruciferin is main seed storage protein in Brassica napus. To gain a better understanding of the Bncru4 promoter function, we conducted the promoter 5′ deletion analysis in transgenic Arabidopsis. In the β-glucuronidase (GUS) expression assay, Bncru4 promoter was strongly active in transgenic seeds. In addition, deletion of RY-elements (−236 bp region) dramatically decreased the promoter activity in seed embryos; however, the GUS expression could be observed in seed coat. Further deletion up to −113 bp region (removed up to the CAAT and TATA box), GUS expression was completely abolished in all tissues. These results were consistent with that of the GUS activity in transgenic seeds. Therefore, we consider that RYelement is crucial to the seed-specific expression of Bncru4 promoter

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration (RDA), Suwon, 441-707, Republic of Korea

    Kyung Hee Roh, Soo Bok Choi, Han-Chul Kang, Jong-Bum Kim, Hyun Uk Kim, Kyeong-Ryeol Lee & Sun Hee Kim

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  1. Kyung Hee Roh
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  2. Soo Bok Choi
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  3. Han-Chul Kang
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  4. Jong-Bum Kim
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  5. Hyun Uk Kim
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  6. Kyeong-Ryeol Lee
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  7. Sun Hee Kim
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Correspondence to Kyung Hee Roh.

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Roh, K.H., Choi, S.B., Kang, HC. et al. Isolation and functional characterization of the Brassica napus cruciferin gene cru4 promoter. J Korean Soc Appl Biol Chem 57, 555–560 (2014). https://doi.org/10.1007/s13765-014-4149-2

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