Abstract
Dollar spot (caused by Sclerotinia homoeocarpa F. T. Bennett) is the most economically important fungal turf disease on golf courses in North America. This disease is mainly controlled by using multiple fungicides. However, the causal fungus has developed resistance to seveal classes of fungicides and host resistance is an alternative control strategy. A cree** bentgrass (Agrostis stolonifera L.) cultivar improved with dollar spot resistance would greatly reduce the costs and environmental impacts of fungicide applications. Our previous QTL analysis in the cree** bentgrass ‘549 × 372’ population detected one QTL with a large effect for dollar spot resistance and several QTLs with smaller effects. One RAPD primer, AW10, amplified an 650-bp band in the parent ‘372’, was named 3.AW10.650. This RAPD marker was located on linkage group 7.1 and was significantly (p < 0.0001) associated with the QTL for dollar spot resistance based on 697 progeny of the ‘549 × 372’ population. In this study, we converted the RAPD marker 3.AW10.650 into a sequence characterized amplified region (SCAR) marker, named CreepDSp.650. The SCAR marker showed a significant association (p = 0.006) with dollar spot resistance in an additional segregating population developed at the University of Illinois. We report here the first PCR-based SCAR marker associated with a major-effect QTL for dollar spot resistance in cree** bentgrass. This SCAR marker will facilitate evaluating relatively large numbers of breeding progeny for marker-assisted selection (MAS) in bentgrasses.
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Chakraborty, N., Sim, SC., Casler, M. et al. Development of SCAR marker for the detection of QTL for resistance to dollar spot in cree** bentgrass (Agrostis stolonifera L.). Hortic. Environ. Biotechnol. 55, 329–334 (2014). https://doi.org/10.1007/s13580-014-0015-8
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DOI: https://doi.org/10.1007/s13580-014-0015-8