SpringerLink
Log in

Differential expression of leaf proteins in four cultivars of peanut (Arachis hypogaea L.) under water stress

  • Original Article
  • Published:
3 Biotech Aims and scope Submit manuscript

Abstract

Drought is a major constraint to the productivity of many crops affecting various physiological and biochemical processes. Seventy percent of the peanuts are grown in semiarid tropics that are frequently prone to drought stress. So, we analyzed its effect in 4 cultivars of peanut, with different degrees of drought tolerance, under 10 and 20 days of water stress using two-dimensional gel electrophoresis and mass spectrometry. A total of 189 differentially expressed protein spots were identified in the leaf proteome of all the 4 cultivars using PD Quest Basic software; 74 in ICGV 91114, 41 in ICGS 76, 44 in J 11 and 30 in JL 24. Of these, 30 protein spots were subjected to in-gel trypsin digestion followed by MALDI-TOF that are functionally categorized into 5 groups: molecular chaperones, signal transducers, photosynthetic proteins, defense proteins and detoxification proteins. Of these, 12 proteins were sequenced. Late embryogenesis abundant protein, calcium ion binding protein, sucrose synthase isoform-1, 17.3 kDa heat shock protein and structural maintenance of chromosome proteins were overexpressed only in the 15 and 20 days stressed plants of ICGV 91114 cultivar while cytosolic ascorbate peroxidase was expressed with varying levels in the 10 and 20 days stressed plants of all the 4 cultivars. Signaling protein like 14-3-3 and defense proteins like alpha-methyl-mannoside-specific lectin and mannose/glucose-binding lectins were differentially expressed in the 4 cultivars. Photosynthetic protein like Rubisco was down-regulated in the stressed plants of all 4 cultivars while Photosystem-I reaction center subunit-II of chloroplast precursor protein was overexpressed in only 20 days stressed plants of ICGV 91114, ICGS 76 and J11 cultivars. These differentially expressed proteins could potentially be used as protein markers for screening the peanut germplasm and further crop improvement.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

Abbreviations

LEA-1:

Late embryogenesis abundant protein-1

CalM42:

Calcium ion binding protein

Susy-1:

Sucrose synthase isoform-1

SMC-1:

Structural maintenance of chromosome-1

APX-1:

Ascorbate peroxidase-1

PS I:

Photosystem-I reaction center subunit-II of chloroplast precursor protein

10 DS:

10 days water-stressed plants

20 DS:

20 Days water-stressed plants

MALDI-TOF:

Matrix-assisted laser desorption/ionization-time-of-flight

References

  • Asada K (2006) Production and Scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiol 141:391–396

    Article  CAS  Google Scholar 

  • Basha SM, Roberts RM (1981) The glycoproteins of plant seeds: analysis by two dimensional polyacrylamide gel electrophoresis and by their lectin-binding properties. Plant Physiol 67:936–939

    Article  CAS  Google Scholar 

  • Basha SM, Katam R, Naik KSS (2007) Differential response of peanut genotypes to water stress. Peanut Sci 34:96–104

    Article  Google Scholar 

  • Bhushan D, Pandey A, Choudhary MK, Datta A, Chakraborty S, Chakraborty N (2007) Comparative proteomics analysis of differentially expressed proteins in chickpea extracellular matrix during dehydration stress. Mol Cell Proteom 6:1868–1884

    Article  CAS  Google Scholar 

  • Boston RS, Viitanen PV, Vierling E (1996) Molecular chaperones and protein folding in plants. Plant Mol Biol 32:191–222

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Bray EA, Bailey-Serres J, Weretilnyk E (2000) Responses to abiotic stress. Biochemistry & molecular biology of plants. In: Gruissem W, Jones R (eds) American society of plant physiologists. Rockville, pp 1158–1203

  • Britt AB (1999) Molecular genetics of DNA repair in higher plants. Trends Plant Sci 4:20–25

    Article  CAS  Google Scholar 

  • Castillejo MA, Maldonado AM, Ogueta S, Jorrin JV (2008) Proteomic analysis of responses to drought stress in sunflower (Helianthus annuus) leaves by 2DE gel electrophoresis and mass spectrometry. Open Proteom J 1:59–71

    Article  CAS  Google Scholar 

  • Chaves MM, Maroco JP, Pereira JS (2003) Understanding plant responses to drought from genes to the whole plant. Funct Plant Biol 30:239–264

    Article  CAS  Google Scholar 

  • Close TJ (1996) Dehydrins: emergence of a biochemical role of a family of plant dehydration proteins. Physiol Plantarum 4:795–803

    Article  Google Scholar 

  • Cooke MS, Evans MD, Dizdaroglu M, Lunec J (2003) Oxidative DNA damage: mechanisms, mutation, and disease. FASEB J 17:1195–1214

    Article  CAS  Google Scholar 

  • Davletova S, Rizhsky L, Liang H, Shenggiang Z, Oliver DJ, Coutu J, Shulaev V, Schlauch K, Mittler R (2005) Cytolosic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis. Plant Cell 17:268–281

    Article  CAS  Google Scholar 

  • De Vetten NC, Ferl RJ (1994) Two genes encoding GF14 (14-3-3) proteins in Zea mays. Plant Physiol 106:1593–1604

    Article  Google Scholar 

  • Dejardin A, Sokolov LN, Kleczkowski LA (1999) Sugar/osmoticum levels modulate differential abscisic acid-independent expression of two stress-responsive sucrose synthase genes in Arabidopsis. Biochem J 344:503–509

    CAS  Google Scholar 

  • Devaiah KM, Bali Geetha, Athmaram TN, Basha MS (2007) Identification of two new genes from drought tolerant peanut up-regulated in response to drought. Plant Growth Regul 52(3):249–258

    Article  CAS  Google Scholar 

  • Drame KN, Clavel D, Repellin A, Passaquet C, Zuily-Fodil Y (2007) Water deficit induces variation in expression of stress responsive genes in two peanut (Arachis hypogaea L) cultivars with different tolerance to drought. Plant Physiol Biochem 45:236–243

    Article  CAS  Google Scholar 

  • Emanuelsson O, Soren B, Gunnar VH, Henrik N (2007) Locating proteins in the cell using Target P, Signal P, and related tools. Nat Protoc 2:953–971

    Article  CAS  Google Scholar 

  • Eriksson J, Chait BT, Fenyo D (2000) A statistical basis for testing the significance of mass spectrometric protein identification results. Anal Chem 72:999–1005

    Article  CAS  Google Scholar 

  • Faghani E, Gharechahi J, Komatsu S, Mirzaei M, Ramzan et al (2015) Comparative physiology and proteomic analysis of two wheat genotypes contrasting in drought tolerance. J Proteom 114:1–15

    Article  CAS  Google Scholar 

  • Farooq M, Basra SMA, Wahid A, Cheema ZA, Cheema MA, Khaliq A (2009) Physiological role of exogenously applied glycinebetaine in improving drought tolerance of fine grain aromatic rice (Oryza sativa L). J Agron Crop Sci 194:325–333

    Article  Google Scholar 

  • Feller U, Anders I, Mae T (2008) Rubiscolytics: fate of Rubisco after its enzymatic function in a cell is terminated. J Exp Bot 59:1615–1624

    Article  CAS  Google Scholar 

  • Ferl RJ (1996) 14-3-3 proteins and signal transduction. Annu Rev Plant Physiol Plant Mol Biol 47:49–73

    Article  CAS  Google Scholar 

  • Govind G, Harshavardhan VT, Patricia JK, Dhanalakshmi R, Senthil KM, Sreenivasulu N, Udayakumar M (2009) Identification and functional validation of a unique set of drought induced genes preferentially expressed in response to gradual water stress in peanut. Mol Genet Genom 281:591–605

    Article  CAS  Google Scholar 

  • Graan T, Boyer JS (1990) Very high CO2 partially restores photosynthesis in sunflower at low water potentials. Planta 181:378–384

    Article  CAS  Google Scholar 

  • Granier F (1988) Extraction of plant proteins for two-dimensional electrophoresis. Electrophoresis 9:712–718

    Article  CAS  Google Scholar 

  • Grimplet J, Wheatley MD, Jouira HB, Deluc LG, Cramer GR, Cushman JC (2009) Proteomic and selected metabolite analysis of grape berry tissues under well watered and water-deficit stress conditions. Proteomics 9(9):2503–2528

    Article  CAS  Google Scholar 

  • Guo BZ, Xu G, Cao YG, Holbrook CC, Lynch RE (2006) Identification and characterization of phospholipase D and its association with drought susceptibilities in peanut (Arachis hypogaea). Planta 223:512–520

    Article  CAS  Google Scholar 

  • Ishida T, Kurata T, Okada K, Wada T (2008) A genetic regulatory network in the development of trichomes and root hairs. Annu Rev Plant Biol 59:365–386

    Article  CAS  Google Scholar 

  • Jain AK, Basha SM, Holbrook CC (2001) Identification of drought responsive transcripts in peanut (Arachis hypogaea L.). Electron J Biotechnol 4(2):59–67

    Article  Google Scholar 

  • Jaleel CA, Gopi R, Sankar B, Gomathinayagam M, Panneerselvam R (2008) Differential responses in water use efficiency in two varieties of Catharanthus roseus under drought stress. Comp Rend Biol 331:42–47

    Article  Google Scholar 

  • Jensen RG, Bahr JT (1977) Ribulose 1,5-bisphosphate carboxylase-oxygenase. Ann Rev Plant Physiol 28:379–400

    Article  CAS  Google Scholar 

  • Katam R, Basha SM, Hemanth KN, Vasanthaiah, Naik KSS (2007) Identification of drought tolerant groundnut genotypes employing proteomics approach. J SAT Agric Res 5(1):1–4

  • Katam R, Basha M, Suravajhala P, Pechan T (2010) Analysis of peanut leaf proteome. J Proteome Res 9:2236–2254

    Article  CAS  Google Scholar 

  • Katam R, Sakata K, Prashanth S, Pechan Tibor, Devaiah M et al (2016) Comparative leaf proteomics of drought-tolerant and -susceptible peanut in response to water stress. J Proteom 143:209–226

    Article  CAS  Google Scholar 

  • Ke Y, Han G, He H, Li J (2009) Differential regulation of proteins and phosphoproteins in rice under drought stress. Biochem Biophys Res Commun 379(1):133–138

    Article  CAS  Google Scholar 

  • Komatsu S, Hossain Z (2013) Organ-specific proteome analysis for identification of abiotic stress response mechanism in crop. Front Plant Sci 4:1–9

    Google Scholar 

  • Kottapalli KR, Rakwal R, Shibato J, Burow G, Tissue D, Burke J, Puppala N, Burow M, Payton P (2009) Physiology and proteomics of the water-deficit stress response in three contrasting peanut genotypes. Plant Cell Environ 32:380–407

    Article  CAS  Google Scholar 

  • Kunert KJ, Vorster BJ, Fenta BA, Kibido T, Dionisio G, Foyer CH (2016) Drought stress responses in soybean roots and nodules. Front Plant Sci 7:1015. https://doi.org/10.3389/fpls.2016.01015

    Article  Google Scholar 

  • Lannoo N, Van Damme EJM (2014) Lectin domains at the frontiers of plant defense. Front Plant Sci 5:397

    Google Scholar 

  • Lauer MJ, Boyer JS (1992) Internal CO2 measures directly in leaves: abscisic acid and low leaf water potential cause opposing effects. Plant Physiol 98:1010–1016

    Article  Google Scholar 

  • Lehmann AR (2005) The role of SMC proteins in the responses to DNA damage. DNA Rep 4:309–314

    Article  CAS  Google Scholar 

  • Lis H, Sharon N (1998) Lectins: carbohydrate-specific proteins that mediate cellular recognition. Chem Rev 98:637–674

    Article  CAS  Google Scholar 

  • Luo M, Liang XQ, Dang P, Holbrook CC, Bausher MG, Lee RD, Guo BZ (2005) Microarray-based screening of differentially expressed genes in peanut in response to Aspergillus parasiticus infection and drought stress. Plant Sci 169:695–703

    Article  CAS  Google Scholar 

  • Mittler R, Vanderauwera S, Gollery M, Van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci 9:490–498

    Article  CAS  Google Scholar 

  • Morris JS, Clark BN, Gutstein HB (2007) Pinnacle: a fast, automatic and accurate method for detecting and quantifying protein spots in 2-dimensional gel electrophoresis data. Bioinformatics 24:529–536

    Article  Google Scholar 

  • Nasmyth K, Haering CH (2005) The structure and function of SMC and kleisin complexes. Annu Rev Biochem 74:595–648

    Article  CAS  Google Scholar 

  • Nautiyal PC, Nageswara Rao RC, Joshi YC (2002) Moisture-deficit-induced changes in leaf-water content, leaf carbon exchange rate and biomass production in groundnut cultivars differing in specific leaf area. Field Crops Res 74:67–79

    Article  Google Scholar 

  • Padmavathi TAV, Rao DM (2013) Differential accumulation of osmolytes in 4 cultivars of Peanut (Arachis hypogaea L.) under drought stress. J Crop Sci Biotechnol 16(2):151–159

    Article  Google Scholar 

  • Park CJ, Seo YS (2015) Heat shock proteins: a review of the molecular chaperones for plant immunity. Plant Pathol J 31(4):323–333. https://doi.org/10.5423/PPJ.RW.08.2015.0150

    Article  Google Scholar 

  • Parry MAJ, Andralojc PJ, Khan V, Lea PJ, Keys AJ (2002) RuBisCO activity: effects of drought stress. Ann Bot 89:833–839

    Article  CAS  Google Scholar 

  • Payton P, Kottapalli KR, Rowland D, Faircloth W, Guo B, Burrow M, Puppala N, Gallo M (2009) Gene expression profiling in peanut using high density oligonucleotide microarrays. BMC Genom 10:265

    Article  Google Scholar 

  • Perkins DN, Pappin DJC, Creasy DM (1999) Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 20:3551–3567

    Article  CAS  Google Scholar 

  • Pruthvi V, Rama N, Govind G, Nataraja KN (2013) Expression analysis of drought stress specific genes in Peanut (Arachis hypogaea L). Physiol Mol Biol Plants 19(2):277–281

    Article  CAS  Google Scholar 

  • Ramanjulu S, Bartels D (2002) Drought and desiccation-induced modulation of gene expression in plants. Plant Cell Environ 25:141–151

    Article  CAS  Google Scholar 

  • Rampino P, Pataleo S, Gerardi C, Mita G, Perrotta C (2006) Drought stress response in wheat: physiological and molecular analysis of resistant and sensitive genotypes. Plant Cell Environ 29:2143–2152

    Article  CAS  Google Scholar 

  • Ritchie SW, Nguyan HT, Holaday AS (1990) Leaf water content and gas exchange parameters of two wheat genotypes differing in drought resistance. Crop Sci 30:105–111

    Article  Google Scholar 

  • Salekdeh GH, Siopongco J, Wade LJ, Ghareyazie B, Bennett J (2002) Proteomic analysis of rice leaves during drought stress and recovery. Proteomics 2:1131–1145

    Article  CAS  Google Scholar 

  • Sanchez-Rodriguez E, Rubio-Wilhelmi M, Cervilla LM, Blasco B, Rios JJ, Rosales MA, Romer L, Ruiz JM (2010) Genotypic differences in some physiological parameters symptomatic for oxidative stress under moderate drought in tomato plants. Plant Sci 178:30–40

    Article  CAS  Google Scholar 

  • Schellmann S, Hulskamp M (2005) Epidermal differentiation: trichomes in Arabidopsis as a model system. Int J Dev Biol 49:579–584

    Article  Google Scholar 

  • Sebkova V, Unger C, Hardegger M, Sturm A (1995) Biochemical, physiological, and molecular characterization of sucrose synthase from Daucus carota. Plant Physiol 108:75–83

    Article  CAS  Google Scholar 

  • Sharp RE, Hsiao TC, Silk WK (1990) Growth of the maize primary root at low water potentials II role of growth and deposition of hexose and potassium in osmotic adjustment. Plant Physiol 93:1337–1346

    Article  CAS  Google Scholar 

  • Strum A, Lienhard S, Schatte S, Hardegger M (1999) Tissue-specific expression of two genes for sucrose synthase in carrot (Daucus carota L). Plant Mol Biol 39:349–360

    Article  Google Scholar 

  • Timperio AM, Eqidi MG, Zolla L (2008) Proteomics applied on plant abiotic stresses: role of heat shock proteins (HSP). J Proteom 71:391

    Article  CAS  Google Scholar 

  • Tunnacliffe A, Wise MJ (2007) The continuing conundrum of the LEA proteins. Naturwissenschaften 94:791–812

    Article  CAS  Google Scholar 

  • Valentovic P, Luxova M, Kolarovic L, Gasparikova O (2006) Effect of osmotic stress on compatible solutes content, membrane stability and water relations in two maize cultivars. Plant Soil Environ 52:186–191

    Article  Google Scholar 

  • Van Breusegem F, Dat J (2006) Reactive oxygen species in plant cell death. Plant Physiol 141:384–390

    Article  Google Scholar 

  • Van Damme EJM, Barre A, Rouge P, Peumans WJ (2004) Cytoplasmic/nuclear plant lectins: a new story. Trends Plant Sci 9:484–489

    Article  Google Scholar 

  • Vierling E (1991) The roles of heat shock proteins in plants. Annu Rev Plant Physiol Plant Mol Biol 42:579–620

    Article  CAS  Google Scholar 

  • Vijayan M, Chandra N (1999) Lectins. Curr Opin Struct Biol 9:707–714

    Article  CAS  Google Scholar 

  • Wang W, Vinocur B, Shoseyov O, Altman A (2004) Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends Plant Sci 9:244–252

    Article  CAS  Google Scholar 

  • Winter H, Huber SC (2000) Regulation of sucrose metabolism in higher plants: localization and regulation of activity of key enzymes. Crit Rev Biochem Mol Biol 35:253–289

    Article  CAS  Google Scholar 

  • Wise MJ, Tunnacliffe A (2004) POPP the question: what do LEA proteins do? Trends Plant Sci 9:13–17

    Article  CAS  Google Scholar 

  • **ong L, Zhu JK (2002) Molecular and genetic aspects of plant responses to osmotic stress. Plant Cell Environ 25:131–140

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by grants from Council of Scientific and Industrial Research (CSIR), New Delhi, India in the form of Fellowship and also University Grants Commission by providing financial assistance through the Major Research Project.

Author information

Authors and Affiliations

  1. Department of Genetics, Osmania University, Hyderabad, India

    Padmavathi A. V. Thangella & Manohar Rao Daggu

  2. National Institute of Nutrition, Hyderabad, India

    Srinivas N. B. S. Pasumarti, Raghu Pullakhandam & Bhanuprakash Reddy Geereddy

  3. Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, USA

    Padmavathi A. V. Thangella

Authors
  1. Padmavathi A. V. Thangella
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Srinivas N. B. S. Pasumarti
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Raghu Pullakhandam
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bhanuprakash Reddy Geereddy
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Manohar Rao Daggu
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

PT, MRD and GBR conceived of this study and designed the experiments. PT isolated proteins from samples, done all proteomics experiments and analyzed the data and PNBS supported experiments. PT and MRD drafted the manuscript and GBR critically evaluated and proof-read the manuscript. RP supported manuscript corrections.

Corresponding author

Correspondence to Padmavathi A. V. Thangella.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 167 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Thangella, P.A.V., Pasumarti, S.N.B.S., Pullakhandam, R. et al. Differential expression of leaf proteins in four cultivars of peanut (Arachis hypogaea L.) under water stress. 3 Biotech 8, 157 (2018). https://doi.org/10.1007/s13205-018-1180-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s13205-018-1180-8

Keywords

Search

Navigation