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UBR5 promotes migration and invasion of glioma cells by regulating the ECRG4/NF-κB pathway

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Abstract

Human glioma is a kind of common and fatal intracranial tumor. Therefore, exploring effective drug targets for glioma is the main task of basic and clinical research. This study investigated the role of ubiquitin protein ligase E3 module N-recognition 5 (UBR5) in glioma and the underlying mechanism. First, TCGA datasets were employed to analyze the expression of UBR5 in glioblastoma or normal tissues. Western blot assay was applied to examine the protein expression level of UBR5 in glioma or adjacent tissues and other cell lines. It was found that UBR5 was highly expressed in glioma tissues and cells. Then, we discovered that UBR5 accelerated the invasion, proliferation, and migration of transfected glioma cells by colony formation, immunofluorescence staining, transwell, and wound healing assays. Furthermore, Western blot analysis revealed that UBR5 promoted the epithelial–mesenchymal transformation of glioma cells and regulated the ECRG4/NF-κB pathway. Finally, animal experiments and IHC analysis suggested that UBR5 promoted tumor growth in U251-MG cell-injected mice. In conclusion, this study found that UBR5 promoted the migration and invasion of glioma cells by regulating the ECRG4/NF-κB pathway.

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Funding

This work was supported by the Medical Science Research Project of Hebei Province (Grant No. 20190063).

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Authors and Affiliations

  1. Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China

    Qiang Wu, Yan Feng, Liqun Wang, **n Liu & Yanan Li

  2. Department of Pathology, Children’s Hospital of Hebei Province, Shijiazhuang, 050031, Hebei, China

    Ling Liu

Authors
  1. Qiang Wu
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  2. Ling Liu
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  3. Yan Feng
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  4. Liqun Wang
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  5. **n Liu
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  6. Yanan Li
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Corresponding author

Correspondence to Liqun Wang.

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Competing interests

The authors state that there are no conflicts of interest to disclose.

Ethics approval

Ethical approval was obtained from the Ethics Committee of the Second Hospital of Hebei Medical University.

Statement of Informed Consent

Written informed consent was obtained from a legally authorized representative(s) for anonymized patient information to be published in this article.

Contribution of authors

Qiang Wu and Ling Liu designed the study, supervised the data collection, Yan Feng analyzed the data, interpreted the data, Liqun Wang, **n Liu, and Yanan Li prepared the manuscript for publication and reviewed the draft of the manuscript. All authors have read and approved the manuscript.

Additional information

Communicated by Sanjeev Galande.

Corresponding editor: Sanjeev Galande

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Wu, Q., Liu, L., Feng, Y. et al. UBR5 promotes migration and invasion of glioma cells by regulating the ECRG4/NF-κB pathway. J Biosci 47, 45 (2022). https://doi.org/10.1007/s12038-022-00280-9

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  • DOI: https://doi.org/10.1007/s12038-022-00280-9

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