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A refined method for ovule culture in sugar beet (Beta vulgaris L.)

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Abstract

Induction of gynogenesis through ovule culture is a valuable tool to produce haploid and doubled haploid plants in sugar beet (Beta vulgaris L.). However, there is still large room for refining the method. In this study we investigated the gynogenic response of cultured ovules of three sugar beet genotypes, the effect of the application to inflorescences of different pretreatments with mannitol at 4ºC and with 5-azacytidine and 2,4-D, and the effect of the use of different basal culture media and sucrose concentrations. The response was evaluated in terms of percentages of induction of gynogenesis, embryogenesis and callogenesis, as well as of regenerated plants. We showed that a pretreatment with 0.5 M mannitol at 4 °C for 4 days, and with 50 µM 5-AzaC for 1 h, notably improved the percentage of embryogenesis and plant regeneration. Besides, the use of MS basal medium and 60 g/L sucrose was also found beneficial. This study provides new ways to improve the efficiency of haploid induction and plant regeneration through ovule culture in sugar beet, and is potentially applicable to ovule culture in other crops.

Key message

This study provides new ways to improve the efficiency of haploid induction and plant regeneration through ovule culture in sugar beet.

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Authors and Affiliations

  1. Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

    Sara Sohrabi & Mohammad Reza Abdollahi

  2. Department of Plant Biotechnology, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

    Asghar Mirzaie-Asl

  3. Sugar Beet Seed Department, Hamedan Agricultural and Natural Resources Research and Education Center, AREEO, Hamedan, Iran

    Hassan Ebrahimi Koulaei

  4. Sugar Beet Seed Institute (SBSI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

    Mohsen Aghaeezadeh

  5. Cell Biology Group - COMAV Institute, Universitat Politècnica de València, Valencia, Spain

    Jose M. Seguí-Simarro

Authors
  1. Sara Sohrabi
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  2. Mohammad Reza Abdollahi
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  3. Asghar Mirzaie-Asl
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  4. Hassan Ebrahimi Koulaei
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  5. Mohsen Aghaeezadeh
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  6. Jose M. Seguí-Simarro
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Contributions

MRA conceived and designed the experiments. SS performed the experiments. SS, MRA, AMA, HEK and MA prepared reagents, materials and analytic tools. MRA, SS and JMSS analyzed the data and wrote the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Mohammad Reza Abdollahi.

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The authors declare no conflict of interest.

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Communicated by Maria Antonietta Germanà.

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Sohrabi, S., Abdollahi, M.R., Mirzaie-Asl, A. et al. A refined method for ovule culture in sugar beet (Beta vulgaris L.). Plant Cell Tiss Organ Cult 146, 259–267 (2021). https://doi.org/10.1007/s11240-021-02065-8

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  • DOI: https://doi.org/10.1007/s11240-021-02065-8

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