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Production of Doubled Haploid Sugar Beet (Beta vulgaris L.) Plants Through Gynogenesis

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Doubled Haploid Technology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2289))

Abstract

Haploid and doubled haploid plant production through unpollinated ovule culture, with diverse benefits and applications, is considered among the most effective and advantageous breeding method for sugar beet (Beta vulgaris L.). It is known that sugar beet is not responsive to androgenesis, which is widely used for most plant species. Sugar beet is a recalcitrant plant in vitro due to the very low spontaneous chromosome doubling and low gynogenesis rate. Thus, a steadily increasing gynogenesis efficiency has always been an important target for an efficient sugar beet breeding program. Given the scarcity of published papers focusing on gynogenesis in sugar beet, this chapter describes haploid and doubled haploid production through ovule culture of unfertilized flowers as a practical method.

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Acknowledgments

The financial support of TUBITAK (The Scientific and Technological Research Council of Turkey, Project No: TOVAG-113O095) is deeply appreciated. Also, the authors AP and FA are grateful for the scholarships they were provided via the same project.

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Authors and Affiliations

  1. Faculty of Science and Arts, Department of Biology, Bolu Abant Izzet Baysal University, Bolu, Turkey

    Songul Gurel, Arman Pazuki, Fatemeh Aflaki & Ekrem Gurel

Authors
  1. Songul Gurel
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  2. Arman Pazuki
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  3. Fatemeh Aflaki
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  4. Ekrem Gurel
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Editors and Affiliations

  1. Cell Biology Group — COMAV Institute, Universitat Politècnica de València, Valencia, Spain

    Jose M. Segui-Simarro

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Gurel, S., Pazuki, A., Aflaki, F., Gurel, E. (2021). Production of Doubled Haploid Sugar Beet (Beta vulgaris L.) Plants Through Gynogenesis. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2289. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1331-3_21

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  • DOI: https://doi.org/10.1007/978-1-0716-1331-3_21

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1330-6

  • Online ISBN: 978-1-0716-1331-3

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