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Protease S of entomopathogenic bacterium Photorhabdus laumondii: expression, purification and effect on greater wax moth Galleria mellonella

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Abstract

Background

Protease S (PrtS) from Photorhabdus laumondii belongs to the group of protealysin-like proteases (PLPs), which are understudied factors thought to play a role in the interaction of bacteria with other organisms. Since P. laumondii is an insect pathogen and a nematode symbiont, the analysis of the biological functions of PLPs using the PrtS model provides novel data on diverse types of interactions between bacteria and hosts.

Methods and results

Recombinant PrtS was produced in Escherichia coli. Efficient inhibition of PrtS activity by photorin, a recently discovered emfourin-like protein inhibitor from P. laumondii, was demonstrated. The Galleria mellonella was utilized to examine the insect toxicity of PrtS and the impact of PrtS on hemolymph proteins in vitro. The insect toxicity of PrtS is reduced compared to protease homologues from non-pathogenic bacteria and is likely not essential for the infection process. However, using proteomic analysis, potential PrtS targets have been identified in the hemolymph.

Conclusions

The spectrum of identified proteins indicates that the function of PrtS is to modulate the insect immune response. Further studies of PLPs’ biological role in the PrtS and P. laumondii model must clarify the details of PrtS interaction with the insect immune system during bacterial infection.

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Data availability

No datasets were generated or analysed during the current study.

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Acknowledgements

Mass spectrometric analysis of products of Abz-RSVIK(Dnp) hydrolysis by PrtSt were performed using the equipment of the Advanced Mass Spectrometry Core Facility at the Skolkovo Institute of Science and Technology (Skoltech, Moscow, Russia).

Funding

This study was financially supported by the Russian Science Foundation, grant no. 24-24-00122.

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Authors and Affiliations

  1. National Research Centre “Kurchatov Institute”, Moscow, Russia

    Anastasia O. Svetlova, Maria A. Karaseva, Igor M. Berdyshev, Ksenia N. Chukhontseva, Sergey V. Kostrov & Ilya V. Demidyuk

  2. Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia

    Olga V. Pobeguts, Maria A. Galyamina & Igor P. Smirnov

  3. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia

    Nikita B. Polyakov

  4. Skolkovo Institute of Science and Technology, Moscow, Russia

    Maria G. Zavialova

  5. Institute of Biomedical Chemistry, Moscow, Russia

    Maria G. Zavialova

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  1. Anastasia O. Svetlova
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Contributions

Conceptualization: Ilya V. Demidyuk; Data curation: Maria A. Karaseva; Formal analysis: Anastasia O. Svetlova, Maria A. Karaseva, Igor M. Berdyshev, Ksenia N. Chukhontseva, Ilya V. Demidyuk; Funding acquisition: Sergey V. Kostrov, Ilya V. Demidyuk; Investigation: Anastasia O. Svetlova, Maria (A) Karaseva, Igor M. Berdyshev, Ksenia N. Chukhontseva, Olga V. Pobeguts, Maria А. Galyamina, Igor P. Smirnov, Nikita (B) Polyakov, Maria G. Zavialova; Methodology: Anastasia O. Svetlova, Maria (A) Karaseva, Igor M. Berdyshev, Ksenia N. Chukhontseva, Olga V. Pobeguts, Maria А. Galyamina, Nikita (B) Polyakov, Maria G. Zavialova, Ilya V. Demidyuk; Project administration: Ilya V. Demidyuk; Supervision: Anastasia O. Svetlova, Sergey V. Kostrov, Ilya V. Demidyuk; Validation: Anastasia O. Svetlova, Maria A. Karaseva; Visualization: Anastasia O. Svetlova, Maria A. Karaseva, Igor M. Berdyshev, Olga V. Pobeguts, Maria А. Galyamina, Igor P. Smirnov, Maria G. Zavialova, Ilya V. Demidyuk; Writing – original draft: Anastasia O. Svetlova, Maria A. Karaseva, Igor M. Berdyshev, Olga V. Pobeguts, Maria А. Galyamina, Igor P. Smirnov, Maria G. Zavialova, Ilya V. Demidyuk; Writing – review & editing: Anastasia O. Svetlova, Maria A. Karaseva, Igor M. Berdyshev, Ksenia N. Chukhontseva, Olga V. Pobeguts, Maria А. Galyamina, Maria G. Zavialova, Sergey V. Kostrov, Ilya V. Demidyuk.

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Correspondence to Ilya V. Demidyuk.

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Svetlova, A.O., Karaseva, M.A., Berdyshev, I.M. et al. Protease S of entomopathogenic bacterium Photorhabdus laumondii: expression, purification and effect on greater wax moth Galleria mellonella. Mol Biol Rep 51, 713 (2024). https://doi.org/10.1007/s11033-024-09654-8

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