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Extracellular hydrolytic enzymes produced by halophilic bacteria and archaea isolated from hypersaline lake

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Abstract

The screening of bacteria and archaea from Chott El Jerid, a hypersaline lake in the south of Tunisia, led to the isolation of 68 extremely halophilic prokaryotes growing in media with 15–25% of salt. Assessment of 68 partial 16S rRNA analyzed by amplified rDNA restriction analysis (ARDRA) revealed 15 different bacterial and archaeal taxonomic groups. Based on ARDRA results, phenotypic and hydrolytic activity tests, 20 archaeal and 6 bacterial isolates were selected for sequencing. The halophilic isolates were identified as members of the genera: Salicola, Bacillus, Halorubrum, Natrinema and Haloterrigena. Most of these isolates are able to produce hydrolytic enzymes such as amylase, protease, lipase, cellulase, xylanase, pectinase and some of them showed combined activities. Natrinema genus is an excellent candidate for lipase production. These results indicated that the extremely halophilic archaea and bacteria from Chott El Jerid are a potential source of hydrolytic enzymes and may possess commercial value.

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Acknowledgements

MBA and NK were supported by the Tunisian Ministry of Higher Education, Scientific Research and Technology fellowship. This work was published with the support of AIRD (JEAI HALOBIOTECH project “Traitement anaérobie des effluents industriels salins et hypersalins par des bioréacteurs membranaires”).

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  1. Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, Sidi Mansour, km 6, BP 1177, 3018, Sfax, Tunisia

    Fatma Karray, Manel Ben Abdallah, Najwa Kallel, Manel Hamza, Manel Fakhfakh & Sami Sayadi

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  1. Fatma Karray
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  2. Manel Ben Abdallah
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Correspondence to Fatma Karray.

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Karray, F., Ben Abdallah, M., Kallel, N. et al. Extracellular hydrolytic enzymes produced by halophilic bacteria and archaea isolated from hypersaline lake. Mol Biol Rep 45, 1297–1309 (2018). https://doi.org/10.1007/s11033-018-4286-5

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