SpringerLink
Log in

Molecular cloning and expression of a novel MYB transcription factor gene in rubber tree

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

MYB family proteins regulate a variety of cellular processes in plants. Tap** panel dryness (TPD) in rubber tree (Hevea brasiliensis Muell. Arg.) affects latex biosynthesis and causes serious losses to rubber producers. In this study, a novel SANT/MYB transcription factor gene down-regulated in TPD rubber tree, named as HbSM1, was isolated from rubber tree. The complete HbSM1 open reading frame (ORF) was 948 bp in length. The deduced HbSM1 protein is 315 amino acids. HbSM1 belonged to 1RMYB subfamily with a single SANT domain. Sequence alignment revealed that HbSM1 had high homology with MYB members from Ricinus communis and Manihot esculenta, with 72 and 78 % identity, respectively. Moreover, HbSM1 shared 56 % identity with Glycine max GmMYB176. Phylogenetic analysis revealed that HbSM1, GmMYB176, rice OsMYBS2, and OsMYBS3 fell into the same cluster with 93 % bootstrap support value. Comparing expression among different tissues demonstrated that HbSM1 was ubiquitously expressed in all tissues, but it appeared to be preferentially expressed in leaf and latex. Furthermore, HbSM1 transcripts were significantly induced by various phytohormones (including gibberellic acid, ethephon, methyl jasmonate, salicylic acid, and abscisic acid) and wounding treatments. These results suggested that HbSM1 might play multiple roles in plant development via different phytohormones signaling pathways.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Feller A, Machemer K, Braun EL, Grotewold E (2011) Evolutionary and comparative analysis of MYB and bHLH plant transcription factors. Plant J 66:94–116

    Article  CAS  PubMed  Google Scholar 

  2. Stracke R, Werber M, Weisshaar B (2001) The R2R3-MYB gene family in Arabidopsis thaliana. Curr Opin Plant Biol 4:447–456

    Article  CAS  PubMed  Google Scholar 

  3. Ogata K, Morikawa S, Nakamura H, Hojo H, Yoshimura S et al (1995) Comparison of the free and DNA-complexed forms of the DNA-binding domain from c-Myb. Nat Struct Biol 2:309–320

    Article  CAS  PubMed  Google Scholar 

  4. Kranz HD, Denekamp M, Greco R, ** H, Leyva A et al (1998) Towards functional characterisation of the members of the R2R3-MYB gene family from Arabidopsis thaliana. Plant J 16:263–276

    Article  CAS  PubMed  Google Scholar 

  5. Rosinski JA, Atchley WR (1998) Molecular evolution of the Myb family of transcription factors: evidence for polyphyletic origin. J Mol Evol 46:74–83

    Article  CAS  PubMed  Google Scholar 

  6. ** H, Martin C (1999) Multifunctionality and diversity within the plant MYB-gene family. Plant Mol Biol 41:577–585

    Article  CAS  PubMed  Google Scholar 

  7. Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C et al (2010) MYB transcription factors in Arabidopsis. Trends Plant Sci 15:573–581

    Article  CAS  PubMed  Google Scholar 

  8. Riechmann JL, Ratcliffe OJ (2000) A genomic perspective on plant transcription factors. Curr Opin Plant Biol 3:423–434

    Article  CAS  PubMed  Google Scholar 

  9. Chen Y, Yang X, He K, Liu M, Li J et al (2006) The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family. Plant Mol Biol 60:107–124

    Article  CAS  Google Scholar 

  10. Du H, Yang S, Liang Z, Feng B, Liu L et al (2012) Genome-wide analysis of the MYB transcription factor superfamily in soybean. BMC Plant Biol 12:106

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  11. Cai H, Tian S, Dong H (2012) Large scale in silico identification of MYB family genes from wheat expressed sequence tags. Mol Biotechnol 52:184–192

    Article  CAS  PubMed  Google Scholar 

  12. Ito M (2005) Conservation and diversification of three-repeat Myb transcription factors in plants. J Plant Res 118:61–69

    Article  CAS  PubMed  Google Scholar 

  13. Alabadi D, Oyama T, Yanovsky MJ, Harmon FG, Mas P et al (2001) Reciprocal regulation between TOC1 and LHY/CCA1 within the Arabidopsis circadian clock. Science 293:880–883

    Article  CAS  PubMed  Google Scholar 

  14. Wada T, Tachibana T, Shimura Y, Okada K (1997) Epidermal cell differentiation in Arabidopsis determined by a Myb homolog, CPC. Science 277:1113–1116

    Article  CAS  PubMed  Google Scholar 

  15. Ye H, Li L, Guo H, Yin Y (2012) MYBL2 is a substrate of GSK3-like kinase BIN2 and acts as a corepressor of BES1 in brassinosteroid signaling pathway in Arabidopsis. Proc Natl Acad Sci U S A 109:20142–20147

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  16. Dubos C, Le Gourrierec J, Baudry A, Huep G, Lanet E et al (2008) MYBL2 is a new regulator of flavonoid biosynthesis in Arabidopsis thaliana. Plant J 55:940–953

    Article  CAS  PubMed  Google Scholar 

  17. Aasland R, Stewart AF, Gibson T (1996) The SANT domain: a putative DNA-binding domain in the SWI-SNF and ADA complexes, the transcriptional co-repressor N-CoR and TFIIIB. Trends Biochem Sci 21:87–88

    CAS  PubMed  Google Scholar 

  18. Grune T, Brzeski J, Eberharter A, Clapier CR, Corona DF et al (2003) Crystal structure and functional analysis of a nucleosome recognition module of the remodeling factor ISWI. Mol Cell 12:449–460

    Article  PubMed  Google Scholar 

  19. Boyer LA, Langer MR, Crowley KA, Tan S, Denu JM et al (2002) Essential role for the SANT domain in the functioning of multiple chromatin remodeling enzymes. Mol Cell 10:935–942

    Article  CAS  PubMed  Google Scholar 

  20. Boyer LA, Latek RR, Peterson CL (2004) The SANT domain: a unique histone-tail-binding module? Nat Rev Mol Cell Biol 5:158–163

    Article  CAS  PubMed  Google Scholar 

  21. Bandurski RS, Teas HJ (1957) Rubber biosynthesis in latex of Hevea brasiliensis. Plant Physiol 32:643–648

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  22. de Faÿ E, Jacob JL (1989) Symptomatology, histological and cytological aspects of the bark dryness disease. In: d’Auzac J, Jacob JL, Chrestin H (eds) Physiology of rubber tree latex. CRC Press, Boca Raton, pp 407–430

    Google Scholar 

  23. Chrestin H (1989) Biochemical aspects of bark dryness induced by overstimulation of rubber tree with Ethrel. In: d’Auzac J, Jacob JL, Chrestin H (eds) Physiology of rubber tree latex. CRC Press, Boca Raton, pp 341–441

    Google Scholar 

  24. Faridah Y, Siti Arija M, Ghandimathi H (1996) Changes in some physiological latex parameters in relation to over exploitation and onset of induced tap** panel dryness. J Nat Rubber Res 10:182–186

    Google Scholar 

  25. Chen S, Peng S, Huang G, Wu K, Fu X et al (2003) Association of decreased expression of a Myb transcription factor with the TPD (tap** panel dryness) syndrome in Hevea brasiliensis. Plant Mol Biol 51:51–58

    Article  CAS  PubMed  Google Scholar 

  26. Venkatachalam P, Thulaseedharan A, Raghothama K (2007) Identification of expression profiles of tap** panel dryness (TPD) associated genes from the latex of rubber tree (Hevea brasiliensis Muell. Arg.). Planta 226:499–515

    Article  CAS  PubMed  Google Scholar 

  27. Venkatachalam P, Thulaseedharan A, Raghothama K (2009) Molecular identification and characterization of a gene associated with the onset of tap** panel dryness (TPD) syndrome in rubber tree (Hevea brasiliensis Muell.) by mRNA differential display. Mol Biotechnol 41:42–52

    Article  CAS  PubMed  Google Scholar 

  28. Li D, Deng Z, Chen C, ** panel dryness from Hevea brasiliensis latex using suppression subtractive hybridization. BMC Plant Biol 10:140

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  29. Peng S, Wu K, Huang G, Chen S (2011) HbMyb1, a Myb transcription factor from Hevea brasiliensis, suppresses stress induced cell death in transgenic tobacco. Plant Physiol Biochem 49:1429–1435

    Article  CAS  PubMed  Google Scholar 

  30. Lee GI, Howe GA (2003) The tomato mutant spr1 is defective in systemin perception and the production of a systemic wound signal for defense gene expression. Plant J 33:567–576

    Article  CAS  PubMed  Google Scholar 

  31. Xu J, Aileni M, Abbagani S, Zhang P (2010) A reliable and efficient method for total RNA isolation from various members of spurge family (Euphorbiaceae). Phytochem Anal 21:395–398

    Article  CAS  PubMed  Google Scholar 

  32. Letunic I, Copley RR, Schmidt S, Ciccarelli FD, Doerks T et al (2004) SMART 4.0: towards genomic data integration. Nucleic Acids Res 32:D142–D144

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  33. Thompson JD, Gibson TJ, Higgins DG (2002) Multiple sequence alignment using ClustalW and ClustalX. Curr Protoc Bioinform Chapter 2: Unit 2 3

  34. Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245

    Article  CAS  PubMed  Google Scholar 

  35. Qin B (2013) The function of Rad6 gene in Hevea brasiliensis extends beyond DNA repair. Plant Physiol Biochem 66:134–140

    Article  CAS  PubMed  Google Scholar 

  36. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−△△CT Method. Methods 25:402–408

    Article  CAS  PubMed  Google Scholar 

  37. Rubio-Somoza I, Martinez M, Diaz I, Carbonero P (2006) HvMCB1, a R1MYB transcription factor from barley with antagonistic regulatory functions during seed development and germination. Plant J 45:17–30

    Article  CAS  PubMed  Google Scholar 

  38. Paz-Ares J, Ghosal D, Wienand U, Peterson PA, Saedler H (1987) The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators. EMBO J 6:3553–3558

    PubMed Central  CAS  PubMed  Google Scholar 

  39. Yi J, Derynck MR, Li X, Telmer P, Marsolais F et al (2010) A single-repeat MYB transcription factor, GmMYB176, regulates CHS8 gene expression and affects isoflavonoid biosynthesis in soybean. Plant J 62:1019–1034

    CAS  PubMed  Google Scholar 

  40. Lu CA, Ho TH, Ho SL, Yu SM (2002) Three novel MYB proteins with one DNA binding repeat mediate sugar and hormone regulation of alpha-amylase gene expression. Plant Cell 14:1963–1980

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  41. Rose A, Meier I, Wienand U (1999) The tomato I-box binding factor LeMYBI is a member of a novel class of myb-like proteins. Plant J 20:641–652

    Article  CAS  PubMed  Google Scholar 

  42. Klee H (2003) Hormones are in the air. Proc Natl Acad Sci U S A 100:10144–10145

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  43. Hao B, Wu J (2000) Laticifer differentiation in Hevea brasiliensis: induction by exogenous jasmonic acid and linolenic acid. Ann Bot 85:37–43

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by the National Natural Science Foundation Program of China (31300503 and 31460197), the Earmarked Fund for Modern Agro-industry Technology Research System (CARS-34-GW8), the Midwest University Project (MWECSP-RT08, ZXBJH-XK004, and ZXBJH-XK005), and Natural Science Foundation of Hainan Province (313064).

Author information

Authors and Affiliations

  1. Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737, Hainan, China

    Bi Qin

  2. College of Environment and Plant Protection, Hainan University, Haikou, 570228, Hainan, China

    Yu Zhang & Meng Wang

Authors
  1. Bi Qin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yu Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Meng Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Meng Wang.

Additional information

Bi Qin and Yu Zhang have contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Qin, B., Zhang, Y. & Wang, M. Molecular cloning and expression of a novel MYB transcription factor gene in rubber tree. Mol Biol Rep 41, 8169–8176 (2014). https://doi.org/10.1007/s11033-014-3717-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-014-3717-1

Keywords

Search

Navigation