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Large Scale In Silico Identification of MYB Family Genes from Wheat Expressed Sequence Tags

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Abstract

The MYB proteins constitute one of the largest transcription factor families in plants. Much research has been performed to determine their structures, functions, and evolution, especially in the model plants, Arabidopsis, and rice. However, this transcription factor family has been much less studied in wheat (Triticum aestivum), for which no genome sequence is yet available. Despite this, expressed sequence tags are an important resource that permits opportunities for large scale gene identification. In this study, a total of 218 sequences from wheat were identified and confirmed to be putative MYB proteins, including 1RMYB, R2R3-type MYB, 3RMYB, and 4RMYB types. A total of 36 R2R3-type MYB genes with complete open reading frames were obtained. The putative orthologs were assigned in rice and Arabidopsis based on the phylogenetic tree. Tissue-specific expression pattern analyses confirmed the predicted orthologs, and this meant that gene information could be inferred from the Arabidopsis genes. Moreover, the motifs flanking the MYB domain were analyzed using the MEME web server. The distribution of motifs among wheat MYB proteins was investigated and this facilitated subfamily classification.

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Acknowledgment

This work was supported by Key Basic Scientific Study and Development Plan (973 plan; grant no. 2012CB114003), Natural Science Foundation (grant no. 31171830), and Jiangsu Provincial Priority Academic Program Development of Higher Education Institutions.

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  1. Ministry of Agriculture of R. P. China Key Laboratory of Monitoring and Management of Crop Pathogens and Insect Pests, Nan**g Agricultural University, Nan**g, 210095, China

    Hongsheng Cai, Shan Tian & Hansong Dong

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  1. Hongsheng Cai
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  2. Shan Tian
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  3. Hansong Dong
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Correspondence to Hansong Dong.

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Cai, H., Tian, S. & Dong, H. Large Scale In Silico Identification of MYB Family Genes from Wheat Expressed Sequence Tags. Mol Biotechnol 52, 184–192 (2012). https://doi.org/10.1007/s12033-011-9486-3

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