Abstract
Whether allicin can suppress the angiogenesis via inhibiting the activity of vascular endothelial cells (VECs) in preventing epidural hypertrophic scars remains unknown. VECs were treated by allicin at a gradient of concentrations. Cell activity was measured by CCK-8 assay, scratch assay and flow cytometry. Reverse-transcription PCR and Western Blot were used to measure the expression levels of relevant genes and proteins. After treated with allicin at concentrations of 0, 25, 50 and 100 mg/L, the viability of VECs significantly decreased at 24 h (p < 0.001*) and 48 h (p < 0.001*), and migration rate significantly decreased in scratch assay (p = 0.017*) and in Transwell assay (p = 0.021*). As the concentrations of allicin increased, the apoptosis rate of VECs rose up (p = 0.018*). There was no significant difference on cell numbers at S phase (p = 0.25), but cell numbers at G1 phase decreased (p = 0.039*) and at G2 phase increased (p = 0.047*). With the increase of allicin concentrations, the ability of tube formation for VECs significantly decreased (p < 0.001*). Comparing with control group, the expression of PCNA and BCL-2 decreased (p < 0.001*), while the expression of BAX increased significantly (p < 0.001*). Regarding to JAK2/STAT3 pathway, the expression levels of JAK3 and STAT3 decreased significantly with the increase of allicin concentrations (p < 0.001*). Allicin can suppress the activity of VECs probably by regulating JAK2/STAT3 pathway.
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This study was funded by Jiangsu Provincial Health and Family Planning Commission (CXTDB2017004)
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This study was funded by Jiangsu Provincial Health and Family Planning Commission (CXTDB2017004).
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H-HS cell culture and experiments, data analysis and statistics, manuscript drafting, the polish of language. X-MF cell culture and experiments, verification of statistics, manuscript revision and format setting. J-CW study design, supervision, experimental technique guidance, the polish of language. JC study design, software and verification of statistics, the polish of language.
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Sun, HH., Feng, XM., Wang, JC. et al. Allicin can suppress the activity of vascular endothelial cells probably by regulating JAK2/STAT3 pathway. Mol Cell Biochem 476, 435–441 (2021). https://doi.org/10.1007/s11010-020-03919-z
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DOI: https://doi.org/10.1007/s11010-020-03919-z