Abstract
The healthy properties of the Mediterranean diet are mainly attributed to the consumption of Extra Virgin Olive Oil (EVOO). Tunisia as an important producer of olive oil is characterized by a diverse varietal heritage. The aim of this work was to identify the chemical and molecular specificities of nineteen Tunisian olive cultivars cultivated under arid conditions. The study of the genetic variability of nineteen accessions of olive cultivars was based on two different molecular markers; Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) using 5 and 9 primers respectively. The accessions have been fingerprinted and all primers produced 179 PCR bands. 36 RAPD and 94 ISSR polymorphic bands were identified. The chemical characterization of EVOOs from the nineteen studied varieties was based on their fatty acids and phenolic profiles. Principal component analysis was accomplished according to the phenolic contents. It allowed distinguishing four groups of cultivars. Total phenols of Dhokar Nafti and Fougi Gtar cultivars reached 1010 mg per kg. For the fatty acid compositions, thirteen compounds were identified and oleic acid was the most abundant one with 80.66 g per 100 g and 79.19 g per 100 g of the total fatty acid composition for the varieties Rakhmi and Boudaoud respectively. Based on stepwise multiple regression analysis (MRA), 57 RAPD and ISSR markers were found associated with different biochemical traits. ISSR marker UBC8071000bp was significantly associated with five traits including oleic acid, linoleic acid, linolenic acid, hydroxytyrosol and tyrosol compounds. It can be used as potential marker in marker-assisted selection for improvement of olive oil quality with high phenolic and fatty acid contents.
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References
Abdelhamid S, Gouta H, Gharsallaoui M et al (2013) A review on current status of olive and olive oil production in Tunisia. J Korean Soc Int Agric 25:351–357. https://doi.org/10.12719/ksia.2013.25.4.351
Abdelhamid S, Omri A, Grati-Kamoun N et al (2017) Molecular characterization and genetic relationships of cultivated Tunisian olive varieties (Olea europaea L.) using SSR markers. J New Sci 40:2175–2185
Afifi A, Clark V (1984) Computer-aided multivariate analysis. Lifetime Learning Publications, Belmont
Akay Ö, Yüksel G (2017) Clustering the mixed panel dataset using gower’s distance and K-prototypes algorithms. Commun Stat Simul Comput 20:22. https://doi.org/10.1080/03610918.2017.1367806
Alowaiesh B, Singh Z, Fang Z, Kailis SG (2018) Harvest time impacts the fatty acid compositions, phenolic compounds and sensory attributes of Frantoio and Manzanilla olive oil. Sci Hortic (Amsterdam) 234:74–80. https://doi.org/10.1016/j.scienta.2018.02.017
Amarowicz R, Carle R, Dongowski G et al (2009) Influence of postharvest processing and storage on the content of phenolic acids and flavonoids in foods. Mol Nutr Food Res 53:151–183. https://doi.org/10.1002/mnfr.200700486
Amiot MJ (2014) Olive oil and health effects: from epidemiological studies to the molecular mechanisms of phenolic fraction. Oilseeds Fats Crop Lipids 21:D512. https://doi.org/10.1051/ocl/2014029
Beltrán G, Del Rio C, Sánchez S, Martínez L (2004) Influence of harvest date and crop yield on the fatty acid composition of virgin olive oils from cv. Picual J Agric Food Chem 52:3434–3440. https://doi.org/10.1021/jf049894n
Ben-Ayed R, Ennouri K, Ben Hassen H et al (2015) Comparison between DNA-based, pomological and chemical markers accomplished by bioinformatic tools to distinguish within Tunisian olive cultivars. J Fundam Appl Sci 7:408–421
Ben-Hassine K, Taamalli A, Ben-Slama M et al (2015) Characterization and preference map** of autochthonous and introduced olive oil cultivars in Tunisia. Eur J Lipid Sci Technol 117:112–121. https://doi.org/10.1002/ejlt.201400049
Corrado G, La Mura M, Ambrosino O et al (2009) Relationships of Campanian olive cultivars: comparative analysis of molecular and phenotypic data. Genome 52:692–700. https://doi.org/10.1139/G09-044
D’Imperio MD, Viscosi V, Scarano M et al (2011) Integration between molecular and morphological markers for the exploitation of olive germoplasm (Olea europaea). Sci Hortic (Amsterdam) 130:229–240. https://doi.org/10.1016/j.scienta.2011.06.050
Díez CM, Moral J, Barranco D, Rallo L (2016) Genetic diversity and conservation of olive genetic resources. In: Ahuja M, Jain S (eds) Genetic diversity and erosion in plants. Sustainable development and biodiversity, vol 8. Springer, Cham. https://doi.org/10.1007/978-3-319-25954-3_10
Ercan AG, Taskin M, Turgut K (2004) Analysis of genetic diversity in Turkish sesame (Sesamum indicum L.) populations using RAPD markers. Genet Resour Crop Evol 51:599–607. https://doi.org/10.1023/B:GRES.0000024651.45623.f2
European Economic Community (1991) COMMISSION REGULATION (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis. Off J Eur Communities L 269:1–15. http://data.europa.eu/eli/reg/1991/2568/oj
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution (N Y) 39:783–791
Ganopoulos IV, Kazantzis K (2011) Genetic diversity, structure and fruit trait associations in Greek sweet cherry cultivars using microsatellite based (SSR/ISSR) and morpho-physiological markers. Euphytica 181:237–251. https://doi.org/10.1007/s10681-011-0416-z
García-Rodríguez R, Belaj A, Romero-Segura C et al (2017) Exploration of genetic resources to improve the functional quality of virgin olive oil. J Funct Foods 38:1–8. https://doi.org/10.1016/j.jff.2017.08.043
Gower J (1971) A general coefficient of similarity and some of its properties. Biometrics 27:857–871
Grati-Kamoun N, Mahmoud FL, Rebaï A et al (2006) Genetic diversity of Tunisian olive tree (Olea europaea L.) cultivars assessed by AFLP markers. Genet Resour Crop Evol 53:265–275. https://doi.org/10.1007/s10722-004-6130-0
Hannachi H, Msallem M, Ben Elhadj S, El Gazzah M (2007) Influence of the geographical locations on the agronomical and technological potentialities of the olive tree (Olea europaea L.) in Tunisia. Comptes Rendus Biol 330:135–142. https://doi.org/10.1016/j.crvi.2006.11.005
International Olive Council (2009) Determination of biophenols in olive oils by HPLC. Int Olive Counc 29:1–8
International Olive Council (2015) Trade standard applying to olive oils and olive-pomace oils. Int Olive Counc 8:1–17
Ipek M, Seker M, Ipek A, Gul MK (2015) Identification of molecular markers associated with fruit traits in olive and assessment of olive core collection with AFLP markers and fruit traits. Genet Mol Res 14:2762–2774. https://doi.org/10.4238/2015.March.31.6
Kumar S, Stecher G, Li M et al (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549. https://doi.org/10.1093/molbev/msy096
Lamboy WF (1993) Computing genetic similarity coefficients from RAPD data: correcting for the effects of PCR artifacts caused by variation in experimental conditions. Genome Res 4:38–43
Maki M, Horie S (1999) Random amplified polymorphic DNA (RAPD) markers reveal less genetic variation in the endangered plant Cerastium fischerianum var. molle than in the widespread conspecific C. fischerianum var. fischerianum (Caryophyllaceae). Mol Ecol 8:145–150. https://doi.org/10.1046/j.1365-294X.1999.00517.x
Martins-lopes P, Lima-Brito J, Gomes S et al (2007) RAPD and ISSR molecular markers in Olea europaea L.: genetic variability and molecular cultivar identification. Genet Resour Crop Evol 54:117–128. https://doi.org/10.1007/s10722-005-2640-7
Mattingly DJ (1988) Oil for export? A comparison of Libyan, Spanish and Tunisian olive oil production in the Roman empire. J Rom Archaeol 1:33–56
Miho H, Díez CM, Mena-Bravo A et al (2018) Cultivar influence on variability in olive oil phenolic profiles determined through an extensive germplasm survey. Food Chem 266:192–199. https://doi.org/10.1016/j.foodchem.2018.06.002
Oğraş ŞŞ, Kaban G, Kaya M (2016) The effects of geographic region, cultivar and harvest year on fatty acid composition of olive Oil. J Oleo Sci 65:889–895. https://doi.org/10.5650/jos.ess15270
Orlandi F, Bonofiglio T, Romano B, Fornaciari M (2012) Qualitative and quantitative aspects of olive production in relation to climate in southern Italy. Sci Hortic (Amsterdam) 138:151–158. https://doi.org/10.1016/j.scienta.2012.02.029
Piroddi M, Albini A, Fabiani R et al (2017) Nutrigenomics of extra-virgin olive oil: a review. BioFactors 43:17–41. https://doi.org/10.1002/biof.1318
Portarena S, Leonardi L, Scartazza A et al (2019) Combining analysis of fatty acid composition and δ13C in extra-virgin olive oils as affected by harvest period and cultivar: possible use in traceability studies. Food Control 105:151–158. https://doi.org/10.1016/j.foodcont.2019.05.029
Przychodzen P, Wyszkowska R, Gorzynik-Debicka M et al (2019) Anticancer potential of oleuropein, the polyphenol of olive oil, with 2-methoxyestradiol, separately or in combination, in human osteosarcoma cells. Anticancer Res 39:1243–1251. https://doi.org/10.21873/anticanres.13234
Ray NB, Hilsabeck KD, Karagiannis TC, McCord DE (2019) Bioactive olive oil polyphenols in the promotion of health. In: The role of functional food security in global health. Elsevier Inc., pp 623–637
Rodrigues N, Casal S, Peres AM et al (2018) Effect of olive trees density on the quality and composition of olive oil from cv. Arbequina Sci Hortic (Amsterdam) 238:222–233. https://doi.org/10.1016/j.scienta.2018.04.059
Sneath PHA, Sokal RR (1973) Numerical taxonomy.The principles and practice of numerical classification. San Francisco
Tamura K, Nei M, Kumar S (2004) Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci U S A 101:11030–11035. https://doi.org/10.1073/pnas.0404206101
Uceda M, Frias L (1975) Harvest dates. Evolution of the fruit oil content, oil composition and oil quality. In: The proceedings of segundo seminario oleicola internacional. Cordoba, Spain, pp 125–130
Wangchamhan T, Chiewchanwattana S, Sunat K (2017) Efficient algorithms based on the k-means and Chaotic League Championship Algorithm for numeric, categorical, and mixed-type data clustering. Expert Syst Appl. https://doi.org/10.1016/j.eswa.2017.08.004
Acknowledgements
We are grateful to Mr. Abdelmajid Yengui and Mr. Massimiliano Pellegrino for providing technical help and support during all the research activities. We thank Dr. Enzo Perri for his hospitality at CREA Research Centre for Olive, Citrus and Tree Fruit of Rende (CS).
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Omri, A., Abdelhamid, S., Benincasa, C. et al. Genetic diversity and association of molecular markers with biochemical traits in Tunisian olive cultivars. Genet Resour Crop Evol 68, 1181–1197 (2021). https://doi.org/10.1007/s10722-020-01058-4
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DOI: https://doi.org/10.1007/s10722-020-01058-4