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Screening of Siderophore-Producing Bacteria and Their Effects on Promoting the Growth of Plants

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Abstract

Iron deficiency is a major global agricultural problem. Siderophores can help organisms to uptake iron in form of siderophore-Fe3+ complexes and then in the cell cytosol, iron is reducted and released in ferrous form. This research aimed to obtain some efficient siderophore-producing bacterial strains and evaluate their plant growth-promoting effects in the iron-deficit environment. Two strains, Brucella sp. E7 and Pseudomonas brassicae W7, were isolated from rhizosphere soil. Both strains could produce maximum siderophores under the optimal conditions. Plant promoting experiment showed that many indicators of Vigna radiata seedling were all increased significantly by strain E7/W7 or the consortium of E7 + W7. Under no-iron and high iron stress, the inoculation treatment also showed growth promotion effects on both Vigna radiata and Lolium multiflorum. These results indicated that the potential ability of strain E7 and W7 in increasing agricultural production as a growth-promoting agent in iron-deficit soil.

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References

  1. Magdalena AB, Mathilde M, Edith J (2019) Chapter five-siderophores: from natural roles to potential applications. J Adv Appl Microbiol 106:193–225

    Google Scholar 

  2. Ellermann M, Arthur JC (2017) Siderophore-mediated iron acquisition and modulation of host-bacterial interactions. Free Radic Biol Med 105:68–78

    CAS  PubMed  Google Scholar 

  3. Ferreira CMH, Vilas-Boas A, Sousa CA, Soares HMVM, Soares EV (2019) Comparison of five bacterial strains producing siderophores with ability to chelate iron under alkaline conditions. J AMB Express 9:78

    Google Scholar 

  4. Bruno GGD, Gibson DM, Krasnoff SB (2015) Intracellular siderophore but not extracellular siderophore is required for full virulence in Metarhizium robertsii. J Fungal Genet Biol 82:56–68

    Google Scholar 

  5. Cornish AS, Page WI (1995) Production of the triacetecholate siderophore protochelin by Azotobacter vinelandii. Biometals 8:332–338

    CAS  Google Scholar 

  6. Etesami H, Emami S, Alikhani HA (2017) Potassium solubilizing bacteria (KSB): mechanisms, promotion of plant growth, and future prospects. J Soil Sci Plant Nutr 17:897–911

    CAS  Google Scholar 

  7. Parmar P (2010) Isolation of potassium solubilizing bacteria and their inoculation effect on growth of wheat. Dissertation, Chaudhary Charan Singh Haryana Agricultural University, Hisar

  8. Kaushal M, Wani SP (2016) Plant-growth-promoting rhizobacteria: drought stress alleviators to ameliorate vegetable production in drylands. Ann Microbiol 66:35–42

    CAS  Google Scholar 

  9. Kaushal M, Wani SP (2016) Rhizobacterial-plant interactions: strategies ensuring plant growth promotion under drought and salinity stress. Agric Ecosyst Environ 231:68–78

    CAS  Google Scholar 

  10. Martins JG, Martin C, Hernandez AL, Maria TB (2018) Azotochelin and N-dihydroxy-N, N’-diisopropylhexanediamide as Fe sources to cucumber plants in hydroponic cultures. Emir J Food Agric 30:65–76

    Google Scholar 

  11. Moon CD, Zhang XX, Matthijs S, Schafer M (2005) Genomic, genetic and structural analysis of pyoverdine-mediated iron acquisition in the plant growth-promoting bacterium Pseudomonas fluorescens SBW25. BMC Microbiol 8:117–121

    Google Scholar 

  12. Storey EP, Boghozian R, Little JL, Chakraborty R (2006) Characterization of “Schizokinen”, a dihydroxamate-type siderophore produced by Rhi-zobiumleguminosarum IARI 917. Biometals 19:637–649

    CAS  PubMed  Google Scholar 

  13. Susilowati LE, Syekhfani S (2014) Characterization of phosphate solubilizing bacteria isolated from Pb contaminated soils and their potential for dissolving tricalcium phosphate. J Degrade Min Lands Manag 1:57–62

    Google Scholar 

  14. Yesica L, Maria G, Viviana C, Gabriela S, Soledad C, Julio R, Maria IS (2018) Production of siderophores by the bacterium Kosakonia radicincitans and its application of phytopathogenic fungi. Bioresour Technol 259:381–387

    Google Scholar 

  15. Harris WR, Amin SA, Kuepper FC, Green DH, Carrano CJ (2007) Borate binding to siderophores: structure and stability. J Am Chem Soc 129:263–271

    Google Scholar 

  16. Graciela MD, de Araceli SP, Vinicius SG, Michele RC (2019) Comparative genomics of Paraburkholderia kururiensis and its potential in bioremediation, biofertilization, and biocontrol of plant pathogens. MicrobiologyOpen 8:112–117

    Google Scholar 

  17. Lane D (1995) 16S 23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, New York, pp 115–175

    Google Scholar 

  18. Kim OS, Cho YJ, Lee K, Yoon SH (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. J Int J Syst Evol Microbiol 62:716–721

    CAS  Google Scholar 

  19. Saitou N (1987) The neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol Biol Evol 4(4):406

    CAS  PubMed  Google Scholar 

  20. Venkat KS, Menon S, Agarwal H, Divya G (2017) Characterization and optimization of bacterium isolated from soil samples for the production of siderophores. J Resour Technol 3:434–439

    Google Scholar 

  21. Schwyn B, Neilands JB (1987) Universal chemical assay for the detection and determination of siderophores. J Anal Biochem 56:47–56

    Google Scholar 

  22. Chaiharn M, Chunhaleuchanon S, Lumyong S (2009) Screening siderophore producing bacteria as a potential biological control agent for fungal rice pathogens in Thailand. World J Microb Biotechnol 25:1919–1928

    Google Scholar 

  23. Li S, Li Y, Leng Y, Zeng X (2019) Nitric oxide donor improves adventitious rooting in mung bean hypocotyl cuttings exposed to cadmium and osmotic stresses. Environ Exp Bot 164:114–123

    CAS  Google Scholar 

  24. Zhao L, Wang F, Zhao J (2013) Identification and functional characteristics of chlorpyrifos-degrading and plant growth promoting bacterium Acinetobacter calcoaceticus: characteristics of chlorpyrifos-degrading. J Basic Microb 54:457–463

    Google Scholar 

  25. Zhao L, Teng S, Liu Y (2002) Characterization of a versatile rhizospheric organism from cucumber identified as Ochrobactrum haematophilum. J Basic Microb 52:232–244

    Google Scholar 

  26. Qi W, Zhao L (2013) Study of the siderophore-producing Trichoderma asperellum Q1 on cucumber growth promotion under salt stress. J Basic Microb 53:132–141

    Google Scholar 

  27. Mutai C, Njuguna J, Ghimire S (2017) Brachiaria grasses (Brachiaria spp.) harbor a diverse bacterial community with multiple attributes beneficial to plant growth and development. Microbiologyopen 6:56–77

    Google Scholar 

  28. Pankaj K, Sachin T, Dhingra GK, Abha S, Kumar PM, Kumar H, Dubey RC, Maheshwari DK (2018) Inoculation of siderophore producing rhizobacteria and their consortium for growth enhancement of wheat plant. Biocatal Agric Biotechnol 15:264–269

    Google Scholar 

  29. Cui MX, Ma XX, Liu QQ (2017) Effects of iron fertilizer siphon infusion drip drying on chlorophyll content and FCR activity in apple leaves. North Hortic 12:31–35

    Google Scholar 

  30. Wang X, Xue L, Chang S, He X, Fan T, Wu J, Niu J, Brown E (2019) Bioremediation and metabolism of clothianidin by mixed bacterial consortia enriched from contaminated soils in Chinese greenhouse. Process Biochem 78:114–122

    CAS  Google Scholar 

  31. Mahanty T, Bhattacharjee S, Goswami M, Bhattacharyya P, Das B, Ghosh A, Tribedi P (2017) Biofertilizers: a potential approach for sustainable agriculture development. Environ Sci Pollut R 24:3315–3335

    CAS  Google Scholar 

  32. Ribeiro M, Simoes M (2019) Siderophores: a novel approach to fight antimicrobial resistance. Rev Biomark Stud Psychiatr Neurodegener Disord 11:99–120

    Google Scholar 

  33. Li F, Duan T, Li Y (2020) Effects of the fungal endophyte Epichloe festucae var. lolii on growth and physiological responses of perennial ryegrass cv. fairway to combined drought and pathogen stresses. Microorganisms. https://doi.org/10.3390/microorganisms8121917

    Article  PubMed  PubMed Central  Google Scholar 

  34. Gu Y F. (2008) Effects of strontium on physiological and biochemical characteristics and mineral element absorption of ryegrass. Dissertation of Zhejiang University

  35. De Serrano LO, Camper AK, Richards AM (2016) An overview of siderophores for iron acquisition in microorganisms living in the extreme. Biometals 29:551–571

    PubMed  Google Scholar 

  36. Sinha AK, Parli Venkateswaran B, Tripathy SC, Sarkar A (2019) Effects of growth conditions on siderophore producing bacteria and siderophore production from Indian ocean sector of Southern Ocean. J Basic Microb 59:859–960

    Google Scholar 

  37. Tailor AJ, Joshi BH (2012) Characterization and optimization of siderophore production from Pseudomonas fluorescens strain isolated from sugarcane rhizosphere. J Environ 6:688–694

    Google Scholar 

  38. Calvente V, de Orellano ME, Sansone G, Benuzzi D (2001) Effect of nitrogen source and pH on siderophore production by Rhodotorula strains and their application to biocontrol of phytopathogenic moulds. J Ind Microbiol Biotechnol 26:226–229

    CAS  PubMed  Google Scholar 

  39. Sharma T, Kumar N, Rai N (2016) Production and optimization of siderophore producing pseudomonas species isolated from Tarai region of Uttarakhand. Int J Pharm Bio Sci 7:306–314

    CAS  Google Scholar 

  40. Tripathi M, Johri BN, Sharma A (2006) Plant growth–promoting pseudomonas sp. strains reduce natural occurrence of anthracnose in soybean (glycine maxl.) in central Himalayan region. Curr Microbiol 52:390–394

    CAS  PubMed  Google Scholar 

  41. Bisen P (2014) Soil biology. Laboratory protocols in applied life sciences, 1st edn. CRC Press, Boca Ratan, FL, USA, pp 771–888

    Google Scholar 

  42. Postle K (1990) Aerobic regulation of the Escherichia coli Ton-B gene by changes in iron availability and the fur locus. J Bacteriol 172:2287–2293

    CAS  PubMed  PubMed Central  Google Scholar 

  43. Oreto F, Valla SMP, Ana GC, Cristina M, Castillo AM (2015) Doubled haploid production from Spanish onion (Allium cepa L.) germplasm: embryogenesis induction, plant regeneration and chromosome doubling. Front Plant Sci 6:33–36

    Google Scholar 

  44. Li DX, Guo YX, Yuan HY, Zhang M (2005) Determination of chlorophyll content in maize. Chin Agric Sci Bull 6:153–155

    Google Scholar 

  45. Noinaj N, Guillier M, Barnard TJ, Buchanan SK (2010) TonB-dependent transporters: regulation, structure, and function. J Annu Rev Microbiol 64(1):43–60

    CAS  Google Scholar 

  46. Braud A, Hoegy F, Jezequel K, Lebeau T (2009) New insights into the metal specificity of the Pseudomonas aeruginosa pyoverdine-iron uptake pathway. Environ Microbiol 11:1079–1091

    CAS  PubMed  Google Scholar 

  47. Naveed M, Mitter B, Reichenauer TG, Wieczorek K (2014) Increased drought stress resilience of maize through endophytic colonization by Burkholderia phytofirmans PsJN and Enterobacter sp. FD17. Environ Exp Bot 97:30–39

    CAS  Google Scholar 

  48. Etesami H, Maheshwari DK (2018) Use of plant growth promoting rhizobacteria (PGPRs) with multiple plant growth promoting traits in stress agriculture: action mechanisms and future prospects. Ecotoxicol Environ Safe 156:225–246

    CAS  Google Scholar 

  49. Verma VC, Singh SK, Prakash S (2011) Biocontrol and plant growth promotion potential of siderophore producing endophytic Streptomyces from Azadirachta indica A Juss. J Basic Microb 51:550–556

    CAS  Google Scholar 

  50. Raymond KN, Dertz EA, Kim SS (2003) Enterobactin: an archetype for microbial iron transport. Proc Natl Acad Sci USA 100:3584–3588

    CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

This study was funded by the Scientific Research Project from Facing Characteristic Discipline of Bei**g Union University (KYDE40201703); Bei**g Key Laboratory of Bioactive Substances and Functional Foods, Bei**g Union University (12213991724010239); the National Natural Science Foundation of China (31860163); Key Research and Development Project of Gansu Province (20YF3NA018).

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Authors and Affiliations

  1. Bei**g Key Laboratory of Bioactive Substances and Functional Foods, Bei**g Union University, Bei**g, 100191, China

    Yaxuan Sun & **aoya Shang

  2. School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, China

    Juanli Wu, Lingui Xue, Si**g Chang, Junbo Niu & Brown Emaneghemi

  3. School of Civil Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, China

    Guangyang Ji

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  1. Yaxuan Sun
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  4. Lingui Xue
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Contributions

Conceived and designed the experiments: YS. Performed the experiments: JW, XS, GJ. Analyzed the data: YS, JN. Contributed reagents/materials/analysis tools: LX. Wrote the paper: JW, BE. Thanks to Dr. GZ for the Language modifications to this article.

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Correspondence to **aoya Shang or Lingui Xue.

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Sun, Y., Wu, J., Shang, X. et al. Screening of Siderophore-Producing Bacteria and Their Effects on Promoting the Growth of Plants. Curr Microbiol 79, 150 (2022). https://doi.org/10.1007/s00284-022-02777-w

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