SpringerLink
Log in

Development of a Broad-Specific Competitive ELISA for First-Generation Cephalosporin Antibiotics in Animal-Derived Foods Samples

  • Published:
Bulletin of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

The abuse of antibiotics, such as the cephalosporins in livestock and aquaculture productions, usually causes the widespread antibiotic resistance due to their growth-promoting effects. In this study, cephalexin was chosen as the hapten molecule to prepare a broad-spectrum rabbit polyclonal antibody for cephalosporin antibiotics. The obtained antibody exhibited broad cross-reactivity ranging from 0.05% to 100% with 10 cephalosporins. Based on this antibody, we developed a broad-specific indirect competitive ELISA (ic-ELISA) for cefalexin, cefradine, cefadroxil and cefazolin with the half maximal inhibitory concentration (IC50) ranging from 0.72 to 2.99 ng/mL in working buffer. For animal-derived food samples with spiked cephalosporins, the ic-ELISA exhibited an excellent recovery ranging from 72.3% to 95.6%. To verify the accuracy of this proposed ic-ELISA, its detection performance was evaluated utilizing the high-performance liquid chromatography with satisfactory results. This study confirmed that: firstly, the prepared antibody can be used as a class-specific recognition element to develop immunoassays for cephalosporin antibiotics; and secondly, the developed ic-ELISA provided a new tool for broad-spectrum detection of first-generation cephalosporins in animal-derived foods.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Adegoke OA, Quadri MO (2016) Novel spectrophotometric determinations of some cephalosporins following azo dye formation with p-dimethylaminobenzaldehyde. Arab J Chem 9(2):S1272–S1282

    Article  CAS  Google Scholar 

  • Bacanli M, Başaran N (2019) Importance of antibiotic residues in animal food. Food Chem Toxicol 125:462–466

    Article  CAS  Google Scholar 

  • Ben Y, Fu C, Hu M, Liu L, Wong M, Zheng C (2019) Human health risk assessment of antibiotic resistance associated with antibiotic residues in the environment: a review. Environ Res 169:483–493

    Article  CAS  Google Scholar 

  • Bora I, Demir AB, Uzun P (2016) Nonconvulsive status epilepticus cases arising in connection with cephalosporins. Epilepsy Behav Case Rep 6:23–27

    Article  Google Scholar 

  • Bremus A, Dietrich R, Dettmar L, Usleber E, Märtlbauer E (2012) A broadly applicable approach to prepare monoclonal anti-cephalosporin antibodies for immunochemical residue determination in milk. Anal Bioanal Chem 403(2):503–515

    Article  CAS  Google Scholar 

  • Chen L, Wang Z, Ferreri M (2009) Cephalexin residue detection in milk and beef by ELISA and colloidal gold based one-step strip assay. Agric Food Chem 57:4674–4679

    Article  CAS  Google Scholar 

  • Chen X, Ye E (2016) Graphene oxide-reinforced hollow fiber solid-phase microextraction coupled with high-performance liquid chromatography for the determination of cephalosporins in milk samples. Food Anal Methods 9:2452–2462

    Article  Google Scholar 

  • Chen T, Cheng G, Ahmed S, Wang Y, Wang X, Hao H, Yuan Z (2017) New methodologies in screening of antibiotic residues in animal-derived foods: biosensors. Talanta 175:435–442

    Article  CAS  Google Scholar 

  • Dillon PP, Daly SJ, Browne JG, Manning BM, Loomans E, Amerongen AV, O’Kennedy R (2003) Application of an immunosensor for the detection of the β-Lactam antibiotic, cephalexin. Food Agric Immunol 15:225–234

    Article  CAS  Google Scholar 

  • Fan Q, Wang X, Zhou Q et al (2010) Synthesis of artificial antigens of diethylstilbestrol and preparation of its antibody. Front Agric China 4(2):188–194

    Article  Google Scholar 

  • Karageorgou EG, Samanidou VF (2010) Application of ultrasound-assisted matrix solid-phase dispersion extraction to the HPLC confirmatory determination of cephalosporin residues in milk. J Sep Sci 33(17/18):2862–2871

    Article  CAS  Google Scholar 

  • Liu Q, Xu L, Ke Y, ** Y, Zhang F, Liang X (2011) Analysis of cephalosporins by hydrophilic interaction chromatography. J Pharm Biomed Anal 54(3):623–628

    Article  CAS  Google Scholar 

  • Li W, Shen H, Hong Y, Zhang Y, Yuan F, Zhang F (2016) Simultaneous determination of 22 cephalosporins drug residues in pork muscle using liquid chromatography–tandem mass spectrometry. J Chromatogr B 1022:298–307

    Article  CAS  Google Scholar 

  • Lu P, Yang S, Hu D, Ding X, Shi M (2013) Synthesis of hapten and development of immunoassay based on monoclonal antibody for the detection of dufulin in agricultural samples. J Agric Food Chem 61(43):10302–10309

    Article  CAS  Google Scholar 

  • Martínez-Rodríguez JE, Barriga FJ, Santamaria J, Iranzo A, Pareja JA, Revilla M, Rosa CR (2001) Nonconvulsive status epilepticus associated with cephalosporins in patients with renal failure. Am J Med 111(2):115–119

    Article  Google Scholar 

  • Menkem ZE, Ngangom BL, Tamunjoh SSA, Boyom FF (2018) Antibiotic residues in food animals: public health concern. Acta Ecol Sin 39(5):411–415

    Article  Google Scholar 

  • Primavera A, Cocito L, Audenino D (2004) Nonconvulsive status epilepticus during cephalosporin therapy. Neuropsychobiology 49:218–222

    Article  Google Scholar 

  • Strasser A, Usleber E, Schneider E et al (2003) Improved enzyme immunoassay for group-specific determination of penicillins in milk. Food Agric Immunol 15(2):135–143

    Article  CAS  Google Scholar 

  • Wang S, Xu B, Zhang Y, He J (2009) Development of enzyme-linked immunosorbent assay (ELISA) for the detection of neomycin residues in pig muscle, chicken muscle, egg, fish, milk and kidney. Meat Sci 82:53–58

    Article  CAS  Google Scholar 

  • Wang Q, Zhao W (2018) Optical methods of antibiotic residues detections: a comprehensive review. Sens Actuators B 269:238–256

    Article  CAS  Google Scholar 

  • Zhang F, Liu B, Sheng W, Zhang Y, Liu Q, Li S, Wang S (2018) Fluoroimmunoassays for the detection of zearalenone in maize using CdTe/CdS/ZnS quantum dots. Food Chem 255:421–428

    Article  CAS  Google Scholar 

  • Zhi ZL, Meyer UJ, Van den Bedem JW et al (2001) Evaluation of an automated and integrated flow-through immunoanalysis system for the rapid determination of cephalexin in raw milk. Anal Chim Acta 442(2):207–219

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was financially supported by Hebei Provincial Science and Technology Department “Study on High-throughput Rapid Immunoassay Technology for Cephalosporins in Animal-derived Foods” (No. 17275502D).

Author information

Authors and Affiliations

  1. College of Food Science and Technology, Hebei Agricultural University, Baoding, 071001, China

    Minxuan Liu, Yaxin Sang, **g Zhang, Jian Li, Wenlong Yu, Fuyuan Zhang & **anghong Wang

Authors
  1. Minxuan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yaxin Sang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. **g Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jian Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wenlong Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Fuyuan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. **anghong Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to **anghong Wang.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liu, M., Sang, Y., Zhang, J. et al. Development of a Broad-Specific Competitive ELISA for First-Generation Cephalosporin Antibiotics in Animal-Derived Foods Samples. Bull Environ Contam Toxicol 107, 215–220 (2021). https://doi.org/10.1007/s00128-020-02885-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00128-020-02885-y

Keywords

Search

Navigation