Abstract
This paper described a combined high-performance liquid chromatography (HPLC) -photochemical spectrofluorimetry/solid-phase extraction (SPE) procedure for determining the concentration of unmetabolized folic acid in human serum. After being extracted by SPE (C18-H) column, the serum samples were irradiated under the UV lamp for 11 h, separated on a C18 HPLC column with methanol/water as the mobile phases and determined by a fluorescence detector. Fluorescence excitation and emission wavelengths were at 280 and 443 nm, respectively. The linear range was 1.9 ~ 8.0 ng/mL. The recovery was from 88.9 to 123.6 % with RSD in the range of 4.1–6.4 %.
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Acknowledgments
This research was supported by the SME Technology Innovation Fund, China (Project No. 10C26211200209).
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Zhou, C., Hu, H., Zhang, TC. (2014). Determination of Folic Acid in Serum by SPE and High Performance Liquid Chromatography with Photochemical Spectrofluorimetry. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 251. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37925-3_199
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DOI: https://doi.org/10.1007/978-3-642-37925-3_199
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