Abstract
Current lipidomics is a modern method of analysis of lipids, important cell constituents found in all microbial cells and fulfilling vital roles as structural components of cell membranes, cell energy storage sources, and in some cases as signaling compounds. In either of its current branches, i.e., shotgun lipidomics and LC-MS lipidomics, it provides a fast and reliable information on the lipids present in microorganisms such as archaea, bacteria, cyanobacteria, algae, and yeast, including those inhabiting unusual (psychrophilic, halophilic, thermophilic, etc.) habitats. The number of lipids and more specifically molecular species of lipids ranges from hundreds to thousands, and lipidomics is thus expected to provide a huge amount of data to be processed and evaluated. Further development of lipidomic analysis can be expected to involve the use of new ionization techniques, e.g., atmospheric pressure photoionization MS or high-resolution mass spectrometry of time-of-flight MS analysis of lipids containing unusual head groups, very-long chain saturated, or very-long chain polyunsaturated fatty acids and the application and use of chemical compounds labeled with stable isotopes in the study of dynamic changes of metabolic pathways.
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The research was supported by GACR project 17-00027S and by Institutional Research Concept RVO61388971.
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Řezanka, T., Kolouchová, I., Gharwalová, L., Palyzová, A., Sigler, K. (2018). Lipidomic Analysis of Lower Organisms. In: Wilkes, H. (eds) Hydrocarbons, Oils and Lipids: Diversity, Origin, Chemistry and Fate. Handbook of Hydrocarbon and Lipid Microbiology . Springer, Cham. https://doi.org/10.1007/978-3-319-54529-5_3-1
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